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Literature DB >> 2826536

Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

M Zerbini¹, M Musiani, G Gentilomi, M La Placa.

Abstract

A rapid, simple dot immunoperoxidase assay for the direct detection of cytomegalovirus in clinical urine samples was developed. The assay was performed on nitrocellulose paper dotted with urine pellets free of cellular debris. Cytomegalovirus was detected with a monoclonal antibody to the capsid antigen, and the complex was visualized by immunoperoxidase staining. Positive reactions appeared as well-defined dark blue spots. Of the 87 urine samples examined, 10 proved positive in the dot immunoperoxidase assay, and 77 proved negative. The results agreed completely with the detection of cytomegalovirus-induced antigens in cell cultures inoculated with clinical specimens.

Entities: Disease

Mesh：

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Year: 1987 PMID： 2826536 PMCID： PMC269439 DOI： 10.1128/jcm.25.11.2197-2199.1987

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

1. Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

Authors: M Musiani; M Zerbini; M La Placa
Journal: J Clin Pathol Date: 1985-10 Impact factor: 3.411

2. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.

Authors: C A Gleaves; T F Smith; E A Shuster; G R Pearson
Journal: J Clin Microbiol Date: 1985-02 Impact factor: 5.948

3. Rapid detection of human cytomegalovirus in the urine of humans.

Authors: G Alpert; M C Mazeron; R Colimon; S Plotkin
Journal: J Infect Dis Date: 1985-09 Impact factor: 5.226

4. Rapid dot-immunobinding assay on nitrocellulose for viral antibodies.

Authors: R L Heberling; S S Kalter
Journal: J Clin Microbiol Date: 1986-01 Impact factor: 5.948

5. Detection of specific immunoglobulin M antibodies to cytomegalovirus by using monoclonal antibody to immunoglobulin M in an indirect immunofluorescence assay.

Authors: M Zerbini; M Musiani; G Gentilomi; M La Placa
Journal: J Clin Microbiol Date: 1986-07 Impact factor: 5.948

Dot immunoperoxidase assay using monoclonal antibody for direct detection of cytomegalovirus in urine samples.

1. Alkaline phosphatase immunoenzymatic staining for detection of antigens induced by cytomegalovirus.

2. Comparison of standard tube and shell vial cell culture techniques for the detection of cytomegalovirus in clinical specimens.

3. Rapid detection of human cytomegalovirus in the urine of humans.

4. Rapid dot-immunobinding assay on nitrocellulose for viral antibodies.

5. Detection of specific immunoglobulin M antibodies to cytomegalovirus by using monoclonal antibody to immunoglobulin M in an indirect immunofluorescence assay.

6. Rapid dot enzyme immunoassay for the detection of antibodies to cytomegalovirus.

7. Detection of cytomegalovirus by ELISA in urine samples is inhibited by beta 2 microglobulin.

8. Tween 20 removes antibodies and other proteins from nitrocellulose.

9. Rapid diagnosis of viral infections by an alkaline phosphatase immunocytochemical method.

10. Detection of Toxoplasma gondii antigens by a dot-immunobinding technique.

1. In situ detection of cytomegalovirus DNA in biopsies of AIDS patients using a hybrido-immunocytochemical assay.

2. Dot immunoperoxidase assay for detection of parvovirus B19 antigens in serum samples.

3. Serodiagnosis of scrub typhus with antigens immobilized on nitrocellulose sheet.