| Literature DB >> 21868676 |
Hee-Sung Park1, Michael J Hohn, Takuya Umehara, Li-Tao Guo, Edith M Osborne, Jack Benner, Christopher J Noren, Jesse Rinehart, Dieter Söll.
Abstract
O-Phosphoserine (Sep), the most abundant phosphoamino acid in the eukaryotic phosphoproteome, is not encoded in the genetic code, but synthesized posttranslationally. Here, we present an engineered system for specific cotranslational Sep incorporation (directed by UAG) into any desired position in a protein by an Escherichia coli strain that harbors a Sep-accepting transfer RNA (tRNA(Sep)), its cognate Sep-tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep). Expanding the genetic code rested on reengineering EF-Tu to relax its quality-control function and permit Sep-tRNA(Sep) binding. To test our system, we synthesized the activated form of human mitogen-activated ERK activating kinase 1 (MEK1) with either one or two Sep residues cotranslationally inserted in their canonical positions (Sep(218), Sep(222)). This system has general utility in protein engineering, molecular biology, and disease research.Entities:
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Year: 2011 PMID： 21868676 PMCID： PMC5547737 DOI： 10.1126/science.1207203
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728