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Literature DB >> 24105099

¹H, ¹³C and ¹⁵N resonance assignments for the full-length mammalian cytochrome b₅ in a membrane environment.

Subramanian Vivekanandan¹, Shivani Ahuja, Sang-Choul Im, Lucy Waskell, Ayyalusamy Ramamoorthy.

Abstract

Microsomal cytochrome b5 plays a key role in the oxidation of a variety of exogenous and endogenous compounds, including drugs, fatty acids, cholesterol and steroid hormones. To better understand its functional properties in a membrane mimic environment, we carried out high-resolution solution NMR studies. Here we report resonance assignments for full-length rabbit cytochrome b5 embedded in dodecylphosphocholine micelles.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2013 PMID： 24105099 PMCID： PMC3981966 DOI： 10.1007/s12104-013-9528-9

Source DB: PubMed Journal: Biomol NMR Assign ISSN： 1874-270X Impact factor: 0.746

24 in total

1. High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b(5).

Authors: S B Mulrooney; L Waskell
Journal: Protein Expr Purif Date: 2000-06 Impact factor: 1.650

2. INEPT-based separated-local-field NMR spectroscopy: a unique approach to elucidate side-chain dynamics of membrane-associated proteins.

Authors: Jiadi Xu; Ronald Soong; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal: J Am Chem Soc Date: 2010-07-28 Impact factor: 15.419

3. Monitoring mobility in the early steps of unfolding: the case of oxidized cytochrome b(5) in the presence of 2 M guanidinium chloride.

Authors: F Arnesano; L Banci; I Bertini; D Koulougliotis; A Monti
Journal: Biochemistry Date: 2000-06-20 Impact factor: 3.162

4. Dimethyl propionate ester heme-containing cytochrome b5: structure and stability.

Authors: L Banci; I Bertini; B R Branchini; P Hajieva; G A Spyroulias; P Turano
Journal: J Biol Inorg Chem Date: 2001-06 Impact factor: 3.358

5. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

6. The role of the length and sequence of the linker domain of cytochrome b5 in stimulating cytochrome P450 2B4 catalysis.

Authors: Thomas A Clarke; Sang-Choul Im; Anil Bidwai; Lucy Waskell
Journal: J Biol Chem Date: 2004-06-12 Impact factor: 5.157

7. Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5.

Authors: Ulrich H N Dürr; Kazutoshi Yamamoto; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal: J Am Chem Soc Date: 2007-05-09 Impact factor: 15.419

8. Cytochrome b5, its functions, structure and membrane topology.

Authors: G Vergéres; L Waskell
Journal: Biochimie Date: 1995 Impact factor: 4.079

Review 9. The many roles of cytochrome b5.

Authors: John B Schenkman; Ingela Jansson
Journal: Pharmacol Ther Date: 2003-02 Impact factor: 12.310

Review 10. The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy.

Authors: Ulrich H N Dürr; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal: Biochim Biophys Acta Date: 2007-08-24

5 in total

¹H, ¹³C and ¹⁵N resonance assignments for the full-length mammalian cytochrome b₅ in a membrane environment.

1. High-level expression in Escherichia coli and purification of the membrane-bound form of cytochrome b(5).

2. INEPT-based separated-local-field NMR spectroscopy: a unique approach to elucidate side-chain dynamics of membrane-associated proteins.

3. Monitoring mobility in the early steps of unfolding: the case of oxidized cytochrome b(5) in the presence of 2 M guanidinium chloride.

4. Dimethyl propionate ester heme-containing cytochrome b5: structure and stability.

5. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

6. The role of the length and sequence of the linker domain of cytochrome b5 in stimulating cytochrome P450 2B4 catalysis.

7. Solid-state NMR reveals structural and dynamical properties of a membrane-anchored electron-carrier protein, cytochrome b5.

8. Cytochrome b5, its functions, structure and membrane topology.

Review 9. The many roles of cytochrome b5.

Review 10. The cytochromes P450 and b5 and their reductases--promising targets for structural studies by advanced solid-state NMR spectroscopy.

1. Proton-detected 2D radio frequency driven recoupling solid-state NMR studies on micelle-associated cytochrome-b(5).

2. Cytochrome-P450-cytochrome-b5 interaction in a membrane environment changes 15N chemical shift anisotropy tensors.

3. Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization.

Review 4. Role of protein-protein interactions in cytochrome P450-mediated drug metabolism and toxicity.

5. Kinetic and Structural Characterization of the Effects of Membrane on the Complex of Cytochrome b ₅ and Cytochrome c.