PURPOSE: To characterize in vivo the transpedicular approach (TA) as an alternative route to study intervertebral disc (IVD) regeneration strategies in a sheep model. METHODS: 48 IVD of 12 sheep were used. TA was performed under fluoroscopy, followed by nucleotomy (2-mm shaver resector). A polyurethane scaffold was used to repair the end-plate. X-ray and MRI images were acquired pre-, intra- and post-operatively (1, 3, 6 months). Complications were recorded. RESULTS: TA was feasible in all animals; nucleus pulposus (NP) from L1 to L5 was accessible. Nucleotomy followed by end-plate repair was achieved. Loss of NP signal intensity was shown in MRI images of the nucleotomy group. CONCLUSIONS: TA is feasible in vivo, repeatable after only a short learning period and safely performed without significant morbidity. This animal model allows the study of IVD degeneration physiopathology and investigation of IVD regeneration techniques in vivo.
PURPOSE: To characterize in vivo the transpedicular approach (TA) as an alternative route to study intervertebral disc (IVD) regeneration strategies in a sheep model. METHODS: 48 IVD of 12 sheep were used. TA was performed under fluoroscopy, followed by nucleotomy (2-mm shaver resector). A polyurethane scaffold was used to repair the end-plate. X-ray and MRI images were acquired pre-, intra- and post-operatively (1, 3, 6 months). Complications were recorded. RESULTS: TA was feasible in all animals; nucleus pulposus (NP) from L1 to L5 was accessible. Nucleotomy followed by end-plate repair was achieved. Loss of NP signal intensity was shown in MRI images of the nucleotomy group. CONCLUSIONS: TA is feasible in vivo, repeatable after only a short learning period and safely performed without significant morbidity. This animal model allows the study of IVD degeneration physiopathology and investigation of IVD regeneration techniques in vivo.
Authors: Alberto Di Martino; Liliana Liverani; Alberto Rainer; Giuseppe Salvatore; Marcella Trombetta; Vincenzo Denaro Journal: Musculoskelet Surg Date: 2011-03-12
Authors: Mauro Alini; Stephen M Eisenstein; Keita Ito; Christopher Little; A Annette Kettler; Koichi Masuda; James Melrose; Jim Ralphs; Ian Stokes; Hans Joachim Wilke Journal: Eur Spine J Date: 2007-07-14 Impact factor: 3.134
Authors: Stefan Schwan; Christopher Ludtka; Ingo Wiesner; Andre Baerthel; Andrea Friedmann; Felix Göhre Journal: Eur Spine J Date: 2017-10-27 Impact factor: 3.134
Authors: James Dowdell; Mark Erwin; Theodoe Choma; Alexander Vaccaro; James Iatridis; Samuel K Cho Journal: Neurosurgery Date: 2017-03-01 Impact factor: 4.654
Authors: Charles-Henri Flouzat-Lachaniette; Nicolas Jullien; Charlie Bouthors; Eric Beohou; Béatrice Laurent; Philippe Bierling; Arnaud Dubory; Hélène Rouard Journal: Int Orthop Date: 2018-05-10 Impact factor: 3.075