Stefan Schwan1,2, Christopher Ludtka3,4,5, Ingo Wiesner6, Andre Baerthel3, Andrea Friedmann3,4, Felix Göhre3,7,8. 1. Translational Centre of Regenerative Medicine TRM, University of Leipzig, Philipp-Rosenthal-Straße 55, 04103, Leipzig, Germany. Stefan.Schwan@imws.fraunhofer.de. 2. Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Str. 1, 06120, Halle (Saale), Germany. Stefan.Schwan@imws.fraunhofer.de. 3. Translational Centre of Regenerative Medicine TRM, University of Leipzig, Philipp-Rosenthal-Straße 55, 04103, Leipzig, Germany. 4. Department of Biological and Macromolecular Materials, Fraunhofer Institute for Microstructure of Materials and Systems IMWS, Walter-Hülse-Str. 1, 06120, Halle (Saale), Germany. 5. Department of Chemical and Biomolecular Engineering, University of Tennessee Knoxville, Knoxville, TN, USA. 6. Department of General, Visceral and Vascular Surgery, BG-Klinik Bergmannstrost, Halle (Saale), Germany. 7. Department of Neurosurgery, BG-Klinik Bergmannstrost, Halle (Saale), Germany. 8. Department of Neurosurgery, Helsinki University Central Hospital, Helsinki University, Helsinki, Finland.
Abstract
PURPOSE: This work describes a minimally invasive damage model for ovine lumbar discs via partial nucleotomy using a posterolateral approach. METHODS: Two cadavers were dissected to analyze the percutaneous corridor. Subsequently, 28 ovine had their annulus fibrosus punctured via awl penetration under fluoroscopic control and nucleus pulposus tissue removed via rongeur. Efficacy was assessed by animal morbidity, ease of access to T12-S1 disc spaces, and production of a mechanical injury as verified by discography, radiography, and histology. RESULTS: T12-S1 were accessible with minimal nerve damage morbidity. Scar tissue sealed the disc puncture site in all animals within 6 weeks, withstanding 1 MP of intradiscal pressure. Partial nucleotomy led to a significant reduction in intervertebral disk height and an increased histological degeneration score. CONCLUSION: Inducing a reproducible injury pattern of disc degeneration required minimal time, effort, and equipment. The posterolateral approach allows operation on several discs within a single surgery and multiple animal surgeries within a single day.
PURPOSE: This work describes a minimally invasive damage model for ovine lumbar discs via partial nucleotomy using a posterolateral approach. METHODS: Two cadavers were dissected to analyze the percutaneous corridor. Subsequently, 28 ovine had their annulus fibrosus punctured via awl penetration under fluoroscopic control and nucleus pulposus tissue removed via rongeur. Efficacy was assessed by animal morbidity, ease of access to T12-S1 disc spaces, and production of a mechanical injury as verified by discography, radiography, and histology. RESULTS: T12-S1 were accessible with minimal nerve damage morbidity. Scar tissue sealed the disc puncture site in all animals within 6 weeks, withstanding 1 MP of intradiscal pressure. Partial nucleotomy led to a significant reduction in intervertebral disk height and an increased histological degeneration score. CONCLUSION: Inducing a reproducible injury pattern of disc degeneration required minimal time, effort, and equipment. The posterolateral approach allows operation on several discs within a single surgery and multiple animal surgeries within a single day.
Authors: V C Duance; J K Crean; T J Sims; N Avery; S Smith; J Menage; S M Eisenstein; S Roberts Journal: Spine (Phila Pa 1976) Date: 1998-12-01 Impact factor: 3.468
Authors: Mauro Alini; Stephen M Eisenstein; Keita Ito; Christopher Little; A Annette Kettler; Koichi Masuda; James Melrose; Jim Ralphs; Ian Stokes; Hans Joachim Wilke Journal: Eur Spine J Date: 2007-07-14 Impact factor: 3.134
Authors: Andrea Friedmann; Andre Baertel; Christine Schmitt; Christopher Ludtka; Javorina Milosevic; Hans-Joerg Meisel; Felix Goehre; Stefan Schwan Journal: Int J Mol Sci Date: 2021-04-19 Impact factor: 5.923