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Literature DB >> 23979577

Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas.

Hui Chiu¹, Hillel T Schwartz, Igor Antoshechkin, Paul W Sternberg.

Abstract

CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.

Entities: Species

Keywords: CRISPR; Caenorhabditis elegans; genome resequencing; reverse genetics; targeted nuclease

Mesh：

Substances：
RNA, Helminth

Year: 2013 PMID： 23979577 PMCID： PMC3813845 DOI： 10.1534/genetics.113.155879

Source DB: PubMed Journal: Genetics ISSN： 0016-6731 Impact factor: 4.562

28 in total

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59 in total

1. Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans.

Authors: Xiangyang Chen; Mu Li; Xuezhu Feng; Shouhong Guang
Journal: Genetics Date: 2015-10-19 Impact factor: 4.562

2. Target specificity of the CRISPR-Cas9 system.

Authors: Xuebing Wu; Andrea J Kriz; Phillip A Sharp
Journal: Quant Biol Date: 2014-06

3. Autism-associated missense genetic variants impact locomotion and neurodevelopment in Caenorhabditis elegans.

Authors: Wan-Rong Wong; Katherine I Brugman; Shayda Maher; Jun Young Oh; Kevin Howe; Mihoko Kato; Paul W Sternberg
Journal: Hum Mol Genet Date: 2019-07-01 Impact factor: 6.150

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Authors: Kent G Golic
Journal: Genetics Date: 2013-10 Impact factor: 4.562

Review 5. Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9.

Authors: Christian Frøkjær-Jensen
Journal: Genetics Date: 2013-11 Impact factor: 4.562

6. Streamlined Genome Engineering with a Self-Excising Drug Selection Cassette.

Authors: Daniel J Dickinson; Ariel M Pani; Jennifer K Heppert; Christopher D Higgins; Bob Goldstein
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Journal: Nat Biotechnol Date: 2014-04-20 Impact factor: 54.908