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Literature DB >> 25722925

Target specificity of the CRISPR-Cas9 system.

Xuebing Wu¹, Andrea J Kriz², Phillip A Sharp³.

Abstract

The CRISPR-Cas9 system, naturally a defense mechanism in prokaryotes, has been repurposed as an RNA-guided DNA targeting platform. It has been widely used for genome editing and transcriptome modulation, and has shown great promise in correcting mutations in human genetic diseases. Off-target effects are a critical issue for all of these applications. Here we review the current status on the target specificity of the CRISPR-Cas9 system.

Entities: Chemical Disease Gene Species

Keywords: CRISPR; Cas9; genome engineering; off-targets; target specificity

Year: 2014 PMID： 25722925 PMCID： PMC4338555 DOI： 10.1007/s40484-014-0030-x

Source DB: PubMed Journal: Quant Biol ISSN： 2095-4689

82 in total

1. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity.

Authors: F Ann Ran; Patrick D Hsu; Chie-Yu Lin; Jonathan S Gootenberg; Silvana Konermann; Alexandro E Trevino; David A Scott; Azusa Inoue; Shogo Matoba; Yi Zhang; Feng Zhang
Journal: Cell Date: 2013-08-29 Impact factor: 41.582

Review 2. CRISPR-based adaptive and heritable immunity in prokaryotes.

Authors: John van der Oost; Matthijs M Jore; Edze R Westra; Magnus Lundgren; Stan J J Brouns
Journal: Trends Biochem Sci Date: 2009-07-29 Impact factor: 13.807

3. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells.

Authors: Xuebing Wu; David A Scott; Andrea J Kriz; Anthony C Chiu; Patrick D Hsu; Daniel B Dadon; Albert W Cheng; Alexandro E Trevino; Silvana Konermann; Sidi Chen; Rudolf Jaenisch; Feng Zhang; Phillip A Sharp
Journal: Nat Biotechnol Date: 2014-04-20 Impact factor: 54.908

4. Multiplexed and programmable regulation of gene networks with an integrated RNA and CRISPR/Cas toolkit in human cells.

Authors: Lior Nissim; Samuel D Perli; Alexandra Fridkin; Pablo Perez-Pinera; Timothy K Lu
Journal: Mol Cell Date: 2014-05-15 Impact factor: 17.970

5. Dynamic imaging of genomic loci in living human cells by an optimized CRISPR/Cas system.

Authors: Baohui Chen; Luke A Gilbert; Beth A Cimini; Joerg Schnitzbauer; Wei Zhang; Gene-Wei Li; Jason Park; Elizabeth H Blackburn; Jonathan S Weissman; Lei S Qi; Bo Huang
Journal: Cell Date: 2013-12-19 Impact factor: 41.582

6. Zinc Finger Targeter (ZiFiT): an engineered zinc finger/target site design tool.

Authors: Jeffry D Sander; Peter Zaback; J Keith Joung; Daniel F Voytas; Drena Dobbs
Journal: Nucleic Acids Res Date: 2007-05-25 Impact factor: 16.971

7. RNA-guided editing of bacterial genomes using CRISPR-Cas systems.

Authors: Wenyan Jiang; David Bikard; David Cox; Feng Zhang; Luciano A Marraffini
Journal: Nat Biotechnol Date: 2013-01-29 Impact factor: 54.908

8. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs.

Authors: Yanfang Fu; Jeffry D Sander; Deepak Reyon; Vincent M Cascio; J Keith Joung
Journal: Nat Biotechnol Date: 2014-01-26 Impact factor: 54.908

9. RNA-guided gene activation by CRISPR-Cas9-based transcription factors.

Authors: Pablo Perez-Pinera; D Dewran Kocak; Christopher M Vockley; Andrew F Adler; Ami M Kabadi; Lauren R Polstein; Pratiksha I Thakore; Katherine A Glass; David G Ousterout; Kam W Leong; Farshid Guilak; Gregory E Crawford; Timothy E Reddy; Charles A Gersbach
Journal: Nat Methods Date: 2013-07-25 Impact factor: 28.547

10. A guide RNA sequence design platform for the CRISPR/Cas9 system for model organism genomes.

Authors: Ming Ma; Adam Y Ye; Weiguo Zheng; Lei Kong
Journal: Biomed Res Int Date: 2013-10-03 Impact factor: 3.411

93 in total

1. Cas9 Variants Expand the Target Repertoire in Caenorhabditis elegans.

Authors: Ryan T Bell; Becky X H Fu; Andrew Z Fire
Journal: Genetics Date: 2015-12-17 Impact factor: 4.562

2. Dynamics of Cas10 Govern Discrimination between Self and Non-self in Type III CRISPR-Cas Immunity.

Authors: Ling Wang; Charlie Y Mo; Michael R Wasserman; Jakob T Rostøl; Luciano A Marraffini; Shixin Liu
Journal: Mol Cell Date: 2018-11-29 Impact factor: 17.970

Review 3. Chromatin regulation at the frontier of synthetic biology.

Authors: Albert J Keung; J Keith Joung; Ahmad S Khalil; James J Collins
Journal: Nat Rev Genet Date: 2015-02-10 Impact factor: 53.242

4. Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis.

Authors: Digvijay Singh; Yanbo Wang; John Mallon; Olivia Yang; Jingyi Fei; Anustup Poddar; Damon Ceylan; Scott Bailey; Taekjip Ha
Journal: Nat Struct Mol Biol Date: 2018-04-05 Impact factor: 15.369

5. Easy In Vitro Synthesis of Optimised Functioning Reporter mRNA from Common eGFP Plasmid.

Authors: Gustavo Torres de Souza; Rafaela Chitarra Rodrigues Hell; Jéssica Fernanda da Silva Souza; Luiz Sérgio de Almeida Camargo
Journal: Mol Biotechnol Date: 2018-10 Impact factor: 2.695

6. Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing Chips.

Authors: Cheulhee Jung; John A Hawkins; Stephen K Jones; Yibei Xiao; James R Rybarski; Kaylee E Dillard; Jeffrey Hussmann; Fatema A Saifuddin; Cagri A Savran; Andrew D Ellington; Ailong Ke; William H Press; Ilya J Finkelstein
Journal: Cell Date: 2017-06-29 Impact factor: 41.582

Review 7. How specific is CRISPR/Cas9 really?

Authors: Henriette O'Geen; Abigail S Yu; David J Segal
Journal: Curr Opin Chem Biol Date: 2015-10-24 Impact factor: 8.822