AIM: To further analyse cancer involvement of basic transcription factor 3 (BTF3) after detection of its upregulation in gastric tumor samples. METHODS: BTF3 transcription rates in human gastric tumor tissue samples (n = 20) and adjacent normal tissue (n = 18) specimens as well as in the gastric cancer cell lines AGS, SGC-7901, MKN-28, MKN-45 and MGC803 were analyzed via quantitative real-time polymerase chain reaction. The effect of stable BTF3 silencing via infection with a small interfering RNA (siRNA)-BTF3 expressing lentivirus on SGC-7901 cells was measured via Western blotting analysis, proliferation assays, cell cycle and apoptosis profiling by flow cytometry as well as colony forming assays with a Cellomic Assay System. RESULTS: A significant higher expression of BTF3 mRNA was detected in tumors compared to normal gastric tissues (P < 0.01), especially in section tissues from female patients compared to male patients, and all tested gastric cancer cell lines expressed high levels of BTF3. From days 1 to 5, the relative proliferation rates of stable BTF3-siRNA transfected SGC7901 cells were 82%, 70%, 57%, 49% and 44% compared to the control, while the percentage of cells arrested in the G1 phase was significantly decreased (P = 0.000) and the percentages of cells in the S (P = 0.031) and G2/M (P = 0.027) phases were significantly increased. In addition, the colony forming tendency was significantly decreased (P = 0.014) and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014) after BTF3 was silenced in SGC7901 cells. CONCLUSION: BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.
AIM: To further analyse cancer involvement of basic transcription factor 3 (BTF3) after detection of its upregulation in gastric tumor samples. METHODS:BTF3 transcription rates in humangastric tumor tissue samples (n = 20) and adjacent normal tissue (n = 18) specimens as well as in the gastric cancer cell lines AGS, SGC-7901, MKN-28, MKN-45 and MGC803 were analyzed via quantitative real-time polymerase chain reaction. The effect of stable BTF3 silencing via infection with a small interfering RNA (siRNA)-BTF3 expressing lentivirus on SGC-7901 cells was measured via Western blotting analysis, proliferation assays, cell cycle and apoptosis profiling by flow cytometry as well as colony forming assays with a Cellomic Assay System. RESULTS: A significant higher expression of BTF3 mRNA was detected in tumors compared to normal gastric tissues (P < 0.01), especially in section tissues from female patients compared to male patients, and all tested gastric cancer cell lines expressed high levels of BTF3. From days 1 to 5, the relative proliferation rates of stable BTF3-siRNA transfected SGC7901 cells were 82%, 70%, 57%, 49% and 44% compared to the control, while the percentage of cells arrested in the G1 phase was significantly decreased (P = 0.000) and the percentages of cells in the S (P = 0.031) and G2/M (P = 0.027) phases were significantly increased. In addition, the colony forming tendency was significantly decreased (P = 0.014) and the apoptosis rate increased from 5.73% to 8.59% (P = 0.014) after BTF3 was silenced in SGC7901 cells. CONCLUSION:BTF3 expression is associated with enhanced cell proliferation, reduced cell cycle regulation and apoptosis and its silencing decreased colony forming and proliferation of gastric cancer cells.
Authors: Mathias Uhlen; Per Oksvold; Linn Fagerberg; Emma Lundberg; Kalle Jonasson; Mattias Forsberg; Martin Zwahlen; Caroline Kampf; Kenneth Wester; Sophia Hober; Henrik Wernerus; Lisa Björling; Fredrik Ponten Journal: Nat Biotechnol Date: 2010-12 Impact factor: 54.908
Authors: Line Roy; Sylvie Laboissière; Eman Abdou; Geneviève Thibault; Nathalie Hamel; Maryam Taheri; Daniel Boismenu; Joël Lanoix; Robert E Kearney; Jacques Paiement Journal: Biochim Biophys Acta Date: 2010-06-04
Authors: Claire R Davies; Joanna S Morris; Matthew R Griffiths; Martin J Page; Andrew Pitt; Torsten Stein; Barry A Gusterson Journal: Proteomics Date: 2006-11 Impact factor: 3.984
Authors: Grace Kusumawidjaja; Hany Kayed; Nathalia Giese; Andrea Bauer; Mert Erkan; Thomas Giese; Jörg D Hoheise; Helmut Friess; Jörg Kleeff Journal: Cancer Biol Ther Date: 2007-03-13 Impact factor: 4.742
Authors: Sue Youn Kim; Jiyeon Yoon; Young San Ko; Mee Soo Chang; Jong-Wan Park; Hee Eun Lee; Min A Kim; Ji Hun Kim; Woo Ho Kim; Byung Lan Lee Journal: BMC Cancer Date: 2011-06-22 Impact factor: 4.430
Authors: Hanna Haveri; Mia Westerholm-Ormio; Katri Lindfors; Markku Mäki; Erkki Savilahti; Leif C Andersson; Markku Heikinheimo Journal: BMC Gastroenterol Date: 2008-04-11 Impact factor: 3.067
Authors: Alexandre R Vieira; Merve Bayram; Figen Seymen; Regina C Sencak; Frank Lippert; Adriana Modesto Journal: Front Physiol Date: 2017-02-22 Impact factor: 4.566
Authors: Maria Kotini; Elias H Barriga; Jonathan Leslie; Marc Gentzel; Verena Rauschenberger; Alexandra Schambony; Roberto Mayor Journal: Nat Commun Date: 2018-09-21 Impact factor: 14.919