| Literature DB >> 23654282 |
Mamoru Komatsu1, Kyoko Komatsu, Hanae Koiwai, Yuuki Yamada, Ikuko Kozone, Miho Izumikawa, Junko Hashimoto, Motoki Takagi, Satoshi Omura, Kazuo Shin-ya, David E Cane, Haruo Ikeda.
Abstract
An industrial microorganism, Streptomyces avermitilis, which is a producer of anthelmintic macrocyclic lactones, avermectins, has been constructed as a versatile model host for heterologous expression of genes encoding secondary metabolite biosynthesis. Twenty of the entire biosynthetic gene clusters for secondary metabolites were successively cloned and introduced into a versatile model host S. avermitilis SUKA17 or 22. Almost all S. avermitilis transformants carrying the entire gene cluster produced metabolites as a result of the expression of biosynthetic gene clusters introduced. A few transformants were unable to produce metabolites, but their production was restored by the expression of biosynthetic genes using an alternative promoter or the expression of a regulatory gene in the gene cluster that controls the expression of biosynthetic genes in the cluster using an alternative promoter. Production of metabolites in some transformants of the versatile host was higher than that of the original producers, and cryptic biosynthetic gene clusters in the original producer were also expressed in a versatile host.Entities:
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Year: 2013 PMID： 23654282 PMCID： PMC3932656 DOI： 10.1021/sb3001003
Source DB: PubMed Journal: ACS Synth Biol ISSN： 2161-5063 Impact factor: 5.110