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Literature DB >> 2349242

Expression of human alpha-globin and mouse/human hybrid beta-globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses.

Abstract

Murine cell lines releasing helper-free recombinant retroviruses containing human alpha-globin and mouse/human hybrid beta-globin genes were generated. The expression of the hybrid beta-globin gene but not the human alpha-globin gene was regulated appropriately in infected mouse erythroid leukemia (MEL) cells. Murine bone marrow cells were infected by coculture with virus-producing cells and transplanted into lethally irradiated syngeneic recipients. Greater than 90% of the spleen colonies (12-15 days), which are derived from hemopoietic multipotential stem cells, showed proviral integration. Various levels of expression of the transduced globin genes were detected in all of the provirus-positive spleen colonies. Proviral sequences and transcripts from the transduced globin genes could also be detected in a few long-term reconstituted recipients in an observation period of 10 months after transplantation.

Entities: Disease Gene Species

Mesh：

Substances：
RNA, Messenger
Globins

Year: 1990 PMID： 2349242 PMCID： PMC54107 DOI： 10.1073/pnas.87.11.4349

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

34 in total

1. Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

Authors: D M Bodine; S Karlsson; A W Nienhuis
Journal: Proc Natl Acad Sci U S A Date: 1989-11 Impact factor: 11.205

2. Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability.

Authors: S J Szilvassy; C C Fraser; C J Eaves; P M Lansdorp; A C Eaves; R K Humphries
Journal: Proc Natl Acad Sci U S A Date: 1989-11 Impact factor: 11.205

3. The "beta-like-globin" gene domain in human erythroid cells.

Authors: D Tuan; W Solomon; Q Li; I M London
Journal: Proc Natl Acad Sci U S A Date: 1985-10 Impact factor: 11.205

4. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors.

Authors: G Keller; C Paige; E Gilboa; E F Wagner
Journal: Nature Date: 1985 Nov 14-20 Impact factor: 49.962

5. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.

Authors: S Wright; A Rosenthal; R Flavell; F Grosveld
Journal: Cell Date: 1984-08 Impact factor: 41.582

6. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

Authors: R Mann; R C Mulligan; D Baltimore
Journal: Cell Date: 1983-05 Impact factor: 41.582

7. Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/Wv mice.

Authors: J E Dick; M C Magli; D Huszar; R A Phillips; A Bernstein
Journal: Cell Date: 1985-08 Impact factor: 41.582

8. A majority of mice show long-term expression of a human beta-globin gene after retrovirus transfer into hematopoietic stem cells.

Authors: M A Bender; R E Gelinas; A D Miller
Journal: Mol Cell Biol Date: 1989-04 Impact factor: 4.272

9. A lethal myeloproliferative syndrome in mice transplanted with bone marrow cells infected with a retrovirus expressing granulocyte-macrophage colony stimulating factor.

Authors: G R Johnson; T J Gonda; D Metcalf; I K Hariharan; S Cory
Journal: EMBO J Date: 1989-02 Impact factor: 11.598

Expression of human alpha-globin and mouse/human hybrid beta-globin genes in murine hemopoietic stem cells transduced by recombinant retroviruses.

1. Combination of interleukins 3 and 6 preserves stem cell function in culture and enhances retrovirus-mediated gene transfer into hematopoietic stem cells.

2. Retrovirus-mediated gene transfer to purified hemopoietic stem cells with long-term lympho-myelopoietic repopulating ability.

3. The "beta-like-globin" gene domain in human erythroid cells.

4. Expression of a foreign gene in myeloid and lymphoid cells derived from multipotent haematopoietic precursors.

5. DNA sequences required for regulated expression of beta-globin genes in murine erythroleukemia cells.

6. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus.

7. Introduction of a selectable gene into primitive stem cells capable of long-term reconstitution of the hemopoietic system of W/Wv mice.

8. A majority of mice show long-term expression of a human beta-globin gene after retrovirus transfer into hematopoietic stem cells.

9. A lethal myeloproliferative syndrome in mice transplanted with bone marrow cells infected with a retrovirus expressing granulocyte-macrophage colony stimulating factor.

10. Erythroid-specific expression of human beta-globin genes in transgenic mice.

Review 1. Prospects for homologous recombination in human gene therapy.

Review 2. Hematopoietic stem cell gene therapy:assessing the relevance of preclinical models.

3. Gene transfer to human cells using retrovirus vectors produced by a new polytropic packaging cell line.

4. Insights into neurogenesis and aging: potential therapy for degenerative disease?

5. Phenotypic correction of factor IX deficiency in skin fibroblasts of hemophilic dogs.

6. Long-term in vivo expression of retrovirus-mediated gene transfer in mouse fibroblast implants.

7. Ectopic lymphokine gene expression in human peripheral blood lymphocytes in vivo.