BACKGROUND: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria. METHODS: In this study, 100 P. falciparum strains merozoite surface protein 1(msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively. RESULTS: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp. CONCLUSION: The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions.
BACKGROUND:Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria. METHODS: In this study, 100 P. falciparum strains merozoite surface protein 1(msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively. RESULTS: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp. CONCLUSION: The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions.
Authors: C T Happi; G O Gbotosho; A Sowunmi; C O Falade; D O Akinboye; L Gerena; D E Kyle; W Milhous; D F Wirth; A M J Oduola Journal: Am J Trop Med Hyg Date: 2004-01 Impact factor: 2.345
Authors: Julie Healer; Vince Murphy; Anthony N Hodder; Rosella Masciantonio; Alan W Gemmill; Robin F Anders; Alan F Cowman; Adrian Batchelor Journal: Mol Microbiol Date: 2004-04 Impact factor: 3.501
Authors: Najia K Ghanchi; Andreas Mårtensson; Johan Ursing; Sana Jafri; Sándor Bereczky; Rabia Hussain; Mohammad A Beg Journal: Malar J Date: 2010-01-01 Impact factor: 2.979
Authors: Muyiwa K Oyebola; Emmanuel T Idowu; Yetunde A Olukosi; Bamidele A Iwalokun; Chimere O Agomo; Olusola O Ajibaye; Monday Tola; Adetoro O Otubanjo Journal: Asian Pac J Trop Biomed Date: 2014-05
Authors: Fehintola V Ajogbasile; Adeyemi T Kayode; Paul E Oluniyi; Kazeem O Akano; Jessica N Uwanibe; Benjamin B Adegboyega; Courage Philip; Oluwagboadurami G John; Philomena J Eromon; George Emechebe; Finimo Finimo; Nnenna Ogbulafor; Nma Jiya; Uche Okafor; Jose Ambe; Robinson D Wammanda; Stephen Oguche; Olugbenga A Mokuolu; Akintunde Sowunmi; Onikepe A Folarin; Christian T Happi Journal: Malar J Date: 2021-05-26 Impact factor: 2.979
Authors: Muyiwa K Oyebola; Emmanuel T Idowu; Haddy Nyang; Yetunde A Olukosi; Olubunmi A Otubanjo; Davis C Nwakanma; Samson T Awolola; Alfred Amambua-Ngwa Journal: Malar J Date: 2014-12-13 Impact factor: 2.979