Literature DB >> 23275515

Upgrading fungal gene expression on demand: improved systems for doxycycline-dependent silencing in Aspergillus fumigatus.

Christoph Helmschrott1, Anna Sasse, Sweta Samantaray, Sven Krappmann, Johannes Wagener.   

Abstract

Conditional gene expression is key for functional studies in any given microorganism. To allow tight regulation in the pathogenic mold Aspergillus fumigatus, improved versions of the doxycycline-dependent Tet-On system were generated by replacing functional elements of the precursor module, thereby circumventing the former problem of leakiness due to intramolecular recombination.

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Year:  2012        PMID： 23275515      PMCID： PMC3591957          DOI： 10.1128/AEM.03626-12

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  15 in total

1.  Deciphering cell wall integrity signalling in Aspergillus fumigatus: identification and functional characterization of cell wall stress sensors and relevant Rho GTPases.

Authors:  Karl Dichtl; Christoph Helmschrott; Franziska Dirr; Johannes Wagener
Journal:  Mol Microbiol       Date:  2012-01-04       Impact factor: 3.501

2.  Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background.

Authors:  Sven Krappmann; Christoph Sasse; Gerhard H Braus
Journal:  Eukaryot Cell       Date:  2006-01

Review 3.  Tetracycline-controlled genetic switches.

Authors:  R Sprengel; M T Hasan
Journal:  Handb Exp Pharmacol       Date:  2007

Review 4.  Tools to study molecular mechanisms of Aspergillus pathogenicity.

Authors:  Sven Krappmann
Journal:  Trends Microbiol       Date:  2006-06-27       Impact factor: 17.079

5.  Gene silencing by RNA interference in the koji mold Aspergillus oryzae.

Authors:  Osamu Yamada; Ryoko Ikeda; Yuka Ohkita; Risa Hayashi; Kazutoshi Sakamoto; Osamu Akita
Journal:  Biosci Biotechnol Biochem       Date:  2007-01-07       Impact factor: 2.043

6.  Exploring the sequence space for tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity.

Authors:  S Urlinger; U Baron; M Thellmann; M T Hasan; H Bujard; W Hillen
Journal:  Proc Natl Acad Sci U S A       Date:  2000-07-05       Impact factor: 11.205

Review 7.  Latest developments and in vivo use of the Tet system: ex vivo and in vivo delivery of tetracycline-regulated genes.

Authors:  Stéphane Y Corbel; Fabio M V Rossi
Journal:  Curr Opin Biotechnol       Date:  2002-10       Impact factor: 9.740

8.  Large-scale essential gene identification in Candida albicans and applications to antifungal drug discovery.

Authors:  Terry Roemer; Bo Jiang; John Davison; Troy Ketela; Karynn Veillette; Anouk Breton; Fatou Tandia; Annie Linteau; Susan Sillaots; Catarina Marta; Nick Martel; Steeve Veronneau; Sebastien Lemieux; Sarah Kauffman; Jeff Becker; Reginald Storms; Charles Boone; Howard Bussey
Journal:  Mol Microbiol       Date:  2003-10       Impact factor: 3.501

9.  Analysis of the regulation of the Aspergillus nidulans acuD gene, encoding isocitrate lyase, by construction of a hybrid promoter.

Authors:  J R De Lucas; S Gregory; G Turner
Journal:  Mol Gen Genet       Date:  1994-06-15

10.  Doxycycline-regulated gene expression in the opportunistic fungal pathogen Aspergillus fumigatus.

Authors:  Keith Vogt; Ruchi Bhabhra; Judith C Rhodes; David S Askew
Journal:  BMC Microbiol       Date:  2005-01-13       Impact factor: 3.605
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  33 in total

1.  Role of actin depolymerizing factor cofilin in Aspergillus fumigatus oxidative stress response and pathogenesis.

Authors:  Xiaodong Jia; Xi Zhang; Yingsong Hu; Mandong Hu; Shuguang Tian; Xuelin Han; Yansong Sun; Li Han
Journal:  Curr Genet       Date:  2017-11-23       Impact factor: 3.886

2.  The Paradoxical Effect of Echinocandins in Aspergillus fumigatus Relies on Recovery of the β-1,3-Glucan Synthase Fks1.

Authors:  Veronika Loiko; Johannes Wagener
Journal:  Antimicrob Agents Chemother       Date:  2017-01-24       Impact factor: 5.191

3.  Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant.

Authors:  Jorge Amich; Michaela Dümig; Gráinne O'Keeffe; Jasmin Binder; Sean Doyle; Andreas Beilhack; Sven Krappmann
Journal:  Infect Immun       Date:  2016-03-24       Impact factor: 3.441

4.  Genetic engineering activates biosynthesis of aromatic fumaric acid amides in the human pathogen Aspergillus fumigatus.

Authors:  Daniel Kalb; Thorsten Heinekamp; Gerald Lackner; Daniel H Scharf; Hans-Martin Dahse; Axel A Brakhage; Dirk Hoffmeister
Journal:  Appl Environ Microbiol       Date:  2014-12-19       Impact factor: 4.792

Review 5.  Aspergillus fumigatus and Aspergillosis in 2019.

Authors:  Jean-Paul Latgé; Georgios Chamilos
Journal:  Clin Microbiol Rev       Date:  2019-11-13       Impact factor: 26.132

Review 6.  Light-regulated promoters for tunable, temporal, and affordable control of fungal gene expression.

Authors:  Kevin K Fuller; Jay C Dunlap; Jennifer J Loros
Journal:  Appl Microbiol Biotechnol       Date:  2018-03-22       Impact factor: 4.813

7.  Long Terminal Repeat Retrotransposon Afut4 Promotes Azole Resistance of Aspergillus fumigatus by Enhancing the Expression of sac1 Gene.

Authors:  Mandong Hu; Zongwei Li; Dingchen Li; Jingya Zhao; Yong Chen; Zelei Wang; Fangyan Chen; Li Han
Journal:  Antimicrob Agents Chemother       Date:  2021-09-13       Impact factor: 5.191

8.  Spt20, a Structural Subunit of the SAGA Complex, Regulates Aspergillus fumigatus Biofilm Formation, Asexual Development, and Virulence.

Authors:  Yuan Chen; James I P Stewart; Shuai Liu; Donald C Sheppard; Ling Lu; Shizhu Zhang
Journal:  Appl Environ Microbiol       Date:  2021-10-20       Impact factor: 5.005

9.  Mutant characterization and in vivo conditional repression identify aromatic amino acid biosynthesis to be essential for Aspergillus fumigatus virulence.

Authors:  Anna Sasse; Stefanie N Hamer; Jorge Amich; Jasmin Binder; Sven Krappmann
Journal:  Virulence       Date:  2015-11-25       Impact factor: 5.882

10.  The C2H2 Transcription Factor SltA Contributes to Azole Resistance by Coregulating the Expression of the Drug Target Erg11A and the Drug Efflux Pump Mdr1 in Aspergillus fumigatus.

Authors:  Wenlong Du; Pengfei Zhai; Tingli Wang; Michael J Bromley; Yuanwei Zhang; Ling Lu
Journal:  Antimicrob Agents Chemother       Date:  2021-03-18       Impact factor: 5.191
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