Literature DB >> 16400185

Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end- joining-deficient genetic background.

Sven Krappmann1, Christoph Sasse, Gerhard H Braus.   

Abstract

The akuA gene encoding the Ku70 component of the nonhomologous end-joining machinery was deleted in the opportunistic pathogen Aspergillus fumigatus. No obvious phenotype could be assessed for the corresponding mutant strain but relative frequencies of homologous recombination were increased as deduced from targeting the laccase-encoding abr2 gene.

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Year:  2006        PMID： 16400185      PMCID： PMC1360265          DOI： 10.1128/EC.5.1.212-215.2006

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  15 in total

1.  Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers.

Authors:  P J Punt; C A van den Hondel
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

3.  Methylcitrate synthase from Aspergillus fumigatus. Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia.

Authors:  Claudia Maerker; Manfred Rohde; Axel A Brakhage; Matthias Brock
Journal:  FEBS J       Date:  2005-07       Impact factor: 5.542

4.  Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue.

Authors:  U Reichard; S Büttner; H Eiffert; F Staib; R Rüchel
Journal:  J Med Microbiol       Date:  1990-12       Impact factor: 2.472

5.  Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase.

Authors:  P J Punt; M A Dingemanse; A Kuyvenhoven; R D Soede; P H Pouwels; C A van den Hondel
Journal:  Gene       Date:  1990-09-01       Impact factor: 3.688

6.  Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation.

Authors:  T Kubodera; N Yamashita; A Nishimura
Journal:  Biosci Biotechnol Biochem       Date:  2000-07       Impact factor: 2.043

7.  Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining.

Authors:  Yuuko Ninomiya; Keiichiro Suzuki; Chizu Ishii; Hirokazu Inoue
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-06       Impact factor: 11.205

Review 8.  Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens.

Authors:  Kevin Kavanagh; Emer P Reeves
Journal:  FEMS Microbiol Rev       Date:  2004-02       Impact factor: 16.408

Review 9.  Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.

Authors:  Katherine A Borkovich; Lisa A Alex; Oded Yarden; Michael Freitag; Gloria E Turner; Nick D Read; Stephan Seiler; Deborah Bell-Pedersen; John Paietta; Nora Plesofsky; Michael Plamann; Marta Goodrich-Tanrikulu; Ulrich Schulte; Gertrud Mannhaupt; Frank E Nargang; Alan Radford; Claude Selitrennikoff; James E Galagan; Jay C Dunlap; Jennifer J Loros; David Catcheside; Hirokazu Inoue; Rodolfo Aramayo; Michael Polymenis; Eric U Selker; Matthew S Sachs; George A Marzluf; Ian Paulsen; Rowland Davis; Daniel J Ebbole; Alex Zelter; Eric R Kalkman; Rebecca O'Rourke; Frederick Bowring; Jane Yeadon; Chizu Ishii; Keiichiro Suzuki; Wataru Sakai; Robert Pratt
Journal:  Microbiol Mol Biol Rev       Date:  2004-03       Impact factor: 11.056

10.  A PCR-based system for highly efficient generation of gene replacement mutants in Ustilago maydis.

Authors:  J Kämper
Journal:  Mol Genet Genomics       Date:  2003-12-12       Impact factor: 3.291
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  102 in total

1.  Validation of a self-excising marker in the human pathogen Aspergillus fumigatus by employing the beta-rec/six site-specific recombination system.

Authors:  Thomas Hartmann; Michaela Dümig; Basem M Jaber; Edyta Szewczyk; Patrick Olbermann; Joachim Morschhäuser; Sven Krappmann
Journal:  Appl Environ Microbiol       Date:  2010-07-23       Impact factor: 4.792

2.  Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus.

Authors:  Kevin K Fuller; Shan Chen; Jennifer J Loros; Jay C Dunlap
Journal:  Eukaryot Cell       Date:  2015-08-28

3.  An efficient fungal RNA-silencing system using the DsRed reporter gene.

Authors:  Danielle Janus; Birgit Hoff; Eckhard Hofmann; Ulrich Kück
Journal:  Appl Environ Microbiol       Date:  2006-12-01       Impact factor: 4.792

4.  Nonhomologous chromosomal integration of foreign DNA is completely dependent on MUS-53 (human Lig4 homolog) in Neurospora.

Authors:  Kazuma Ishibashi; Keiichiro Suzuki; Yoshinori Ando; Chihiro Takakura; Hirokazu Inoue
Journal:  Proc Natl Acad Sci U S A       Date:  2006-09-26       Impact factor: 11.205

Review 5.  Aspergillus fumigatus: principles of pathogenesis and host defense.

Authors:  Tobias M Hohl; Marta Feldmesser
Journal:  Eukaryot Cell       Date:  2007-09-21

6.  Gene deletion and allelic replacement in the filamentous fungus Podospora anserina.

Authors:  Riyad El-Khoury; Carole H Sellem; Evelyne Coppin; Antoine Boivin; Marc F P M Maas; Robert Debuchy; Annie Sainsard-Chanet
Journal:  Curr Genet       Date:  2008-02-12       Impact factor: 3.886

Review 7.  Generating and testing molecular hypotheses in the dermatophytes.

Authors:  Theodore C White; Brian G Oliver; Yvonne Gräser; Matthew R Henn
Journal:  Eukaryot Cell       Date:  2008-06-06

8.  Fungal functional genomics: tunable knockout-knock-in expression and tagging strategies.

Authors:  Luis F Larrondo; Hildur V Colot; Christopher L Baker; Jennifer J Loros; Jay C Dunlap
Journal:  Eukaryot Cell       Date:  2009-03-13

9.  Generation of large chromosomal deletions in koji molds Aspergillus oryzae and Aspergillus sojae via a loop-out recombination.

Authors:  Tadashi Takahashi; Feng Jie Jin; Misao Sunagawa; Masayuki Machida; Yasuji Koyama
Journal:  Appl Environ Microbiol       Date:  2008-10-24       Impact factor: 4.792

10.  Analysis of promoter function in Aspergillus fumigatus.

Authors:  Sanjoy Paul; J Stacey Klutts; W Scott Moye-Rowley
Journal:  Eukaryot Cell       Date:  2012-07-27
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