Literature DB >> 3902883

Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens.

J B Mahony, M A Chernesky.   

Abstract

We evaluated various swabs for the recovery of Chlamydia trachomatis and found that only 7 of 14 swabs tested resulted in a greater than 50% recovery of organisms. Storage of chlamydial specimens at 4 degrees C for more than 1 day resulted in a rapid loss of viability, whereas freezing of specimens at -70 degrees C resulted in only a marginal decrease in the isolation rate.

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Year:  1985        PMID： 3902883      PMCID： PMC268547          DOI： 10.1128/jcm.22.5.865-867.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Incubation of swab materials with herpes simplex virus.

Authors:  L R Crane; P A Gutterman; T Chapel; A M Lerner
Journal:  J Infect Dis       Date:  1980-04       Impact factor: 5.226

2.  Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation.

Authors:  F B Gordon; I A Harper; A L Quan; J D Treharne; R S Dwyer; J A Garland
Journal:  J Infect Dis       Date:  1969-10       Impact factor: 5.226

3.  Survival of Ureaplasma urealyticum on different kinds of swabs.

Authors:  S A Poulin; R B Kundsin; H W Horne
Journal:  J Clin Microbiol       Date:  1979-10       Impact factor: 5.948

4.  Survival of Chlamydia trachomatis in different transport media and at different temperatures: diagnostic implications.

Authors:  K H Tjiam; B Y van Heijst; J C de Roo; A de Beer; T van Joost; M F Michel; E Stolz
Journal:  Br J Vener Dis       Date:  1984-04

5.  Laboratory procedures for the isolation of chlamydia trachomatis from the human genital tract.

Authors:  P Reeve; J Owen; J D Oriel
Journal:  J Clin Pathol       Date:  1975-11       Impact factor: 3.411

6.  Provision of a chlamydial culture service to a sexually transmitted diseases clinic.

Authors:  G L Ridgway; V Moss; G Mumtaz; W Atia; A M Emmerson; J D Oriel
Journal:  Br J Vener Dis       Date:  1982-08

7.  Taking cell cultures to the patient in an attempt to improve chlamydial isolation.

Authors:  Y F Ngeow; P E Munday; R T Evans; D Taylor-Robinson
Journal:  Br J Vener Dis       Date:  1981-02

8.  Evaluation of calcium alginate-tipped aluminum swabs transported in Culturettes containing ampules of 2-sucrose phosphate medium for recovery of Chlamydia trachomatis.

Authors:  T F Smith; L A Weed
Journal:  Am J Clin Pathol       Date:  1983-08       Impact factor: 2.493

9.  Toxic effect of sampling swabs and transportation test tubes on the formation of intracytoplasmic inclusions of Chlamydia trachomatis in McCoy cell cultures.

Authors:  P A Mårdh; B Zeeberg
Journal:  Br J Vener Dis       Date:  1981-08
  9 in total
  43 in total

1.  Confirmatory polymerase chain reaction testing for Chlamydia trachomatis in first-void urine from asymptomatic and symptomatic men.

Authors:  J B Mahony; K E Luinstra; J W Sellors; D Jang; M A Chernesky
Journal:  J Clin Microbiol       Date:  1992-09       Impact factor: 5.948

2.  Accuracy of two enzyme immunoassays and cell culture in the detection of Chlamydia trachomatis in low and high risk populations in Senegal.

Authors:  E Van Dyck; N Samb; A D Sarr; L Van de Velden; J Moran; S Mboup; I Ndoye; J L Lamboray; A Meheus; P Piot
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-06       Impact factor: 3.267

3.  A comparison of cytobrush and cotton swab sampling for the detection of Chlamydia trachomatis by cell culture.

Authors:  M I Lees; D M Newnan; M Plackett; P W Traynor; J R Forsyth; S M Garland
Journal:  Genitourin Med       Date:  1990-08

Review 4.  Laboratory techniques for the diagnosis of chlamydial infections.

Authors:  D Taylor-Robinson; B J Thomas
Journal:  Genitourin Med       Date:  1991-06

5.  Comparison of plasmid- and chromosome-based polymerase chain reaction assays for detecting Chlamydia trachomatis nucleic acids.

Authors:  J B Mahony; K E Luinstra; J W Sellors; M A Chernesky
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

6.  Quantitation of Chlamydia trachomatis by culture, direct immunofluorescence and competitive polymerase chain reaction.

Authors:  E H Frost; S Deslandes; D Bourgaux-Ramoisy; P Bourgaux
Journal:  Genitourin Med       Date:  1995-08

7.  Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.

Authors:  Laura van Dommelen; Petra F G Wolffs; Frank H van Tiel; Nicole Dukers; Selma B Herngreen; Cathrien A Bruggeman; Christian J P A Hoebe
Journal:  J Clin Microbiol       Date:  2012-12-19       Impact factor: 5.948

8.  Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults.

Authors:  J B Mahony; M A Chernesky; K Bromberg; J Schachter
Journal:  J Clin Microbiol       Date:  1986-11       Impact factor: 5.948

9.  Nasopharyngeal swabs and nasopharyngeal aspirates equally effective for the diagnosis of viral respiratory disease in hospitalized children.

Authors:  H Frayha; S Castriciano; J Mahony; M Chernesky
Journal:  J Clin Microbiol       Date:  1989-06       Impact factor: 5.948

10.  Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens.

Authors:  J A Kluytmans; W H Goessens; J W Mouton; J H van Rijsoort-Vos; H G Niesters; W G Quint; L Habbema; E Stolz; J H Wagenvoort
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948
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