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Literature DB >> 23150874

Human TNRC6A is an Argonaute-navigator protein for microRNA-mediated gene silencing in the nucleus.

Kenji Nishi¹, Ai Nishi, Tatsuya Nagasawa, Kumiko Ui-Tei.

Abstract

GW182 family proteins play important roles in microRNA (miRNA)-mediated gene silencing. They interact with Argonaute (Ago) proteins and localize in processing bodies, which are cytoplasmic foci involved in mRNA degradation and storage. Here, we demonstrated that human GW182 paralog, TNRC6A, is a nuclear-cytoplasmic shuttling protein, and its subcellular localization is conducted by a nuclear export signal (NES) and a nuclear localization signal (NLS) identified in this study. TNRC6A with mutations in its NES region was predominantly localized in the nucleus in an Ago-independent manner. However, it was found that TNRC6A could bring Ago protein into the nucleus via its Ago-interacting motif(s). Furthermore, miRNAs were also colocalized with nuclear TNRC6A-Ago and exhibited gene silencing activity. These results proposed the possibility that TNRC6A plays an important role in navigating Ago protein into the nucleus to lead miRNA-mediated gene silencing.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2012 PMID： 23150874 PMCID： PMC3527724 DOI： 10.1261/rna.034769.112

Source DB: PubMed Journal: RNA ISSN： 1355-8382 Impact factor: 4.942

62 in total

1. A hexanucleotide element directs microRNA nuclear import.

Authors: Hun-Way Hwang; Erik A Wentzel; Joshua T Mendell
Journal: Science Date: 2007-01-05 Impact factor: 47.728

2. Let-7 microRNA-mediated mRNA deadenylation and translational repression in a mammalian cell-free system.

Authors: Motoaki Wakiyama; Koji Takimoto; Osamu Ohara; Shigeyuki Yokoyama
Journal: Genes Dev Date: 2007-08-01 Impact factor: 11.361

3. Promoter-associated RNA is required for RNA-directed transcriptional gene silencing in human cells.

Authors: Jiang Han; Daniel Kim; Kevin V Morris
Journal: Proc Natl Acad Sci U S A Date: 2007-07-17 Impact factor: 11.205

4. Cellular internalization of green fluorescent protein fused with herpes simplex virus protein VP22 via a lipid raft-mediated endocytic pathway independent of caveolae and Rho family GTPases but dependent on dynamin and Arf6.

Authors: Kenji Nishi; Kaoru Saigo
Journal: J Biol Chem Date: 2007-07-20 Impact factor: 5.157

Review 5. GW182 family proteins are crucial for microRNA-mediated gene silencing.

Authors: Lei Ding; Min Han
Journal: Trends Cell Biol Date: 2007-09-04 Impact factor: 20.808

6. MicroRNAs with a nucleolar location.

Authors: Joan C Ritland Politz; Eric M Hogan; Thoru Pederson
Journal: RNA Date: 2009-07-23 Impact factor: 4.942

7. The silencing domain of GW182 interacts with PABPC1 to promote translational repression and degradation of microRNA targets and is required for target release.

Authors: Latifa Zekri; Eric Huntzinger; Susanne Heimstädt; Elisa Izaurralde
Journal: Mol Cell Biol Date: 2009-09-21 Impact factor: 4.272

8. Mammalian miRNA RISC recruits CAF1 and PABP to affect PABP-dependent deadenylation.

Authors: Marc R Fabian; Géraldine Mathonnet; Thomas Sundermeier; Hansruedi Mathys; Jakob T Zipprich; Yuri V Svitkin; Fabiola Rivas; Martin Jinek; James Wohlschlegel; Jennifer A Doudna; Chyi-Ying A Chen; Ann-Bin Shyu; John R Yates; Gregory J Hannon; Witold Filipowicz; Thomas F Duchaine; Nahum Sonenberg
Journal: Mol Cell Date: 2009-08-27 Impact factor: 17.970

9. Argonaute HITS-CLIP decodes microRNA-mRNA interaction maps.

Authors: Sung Wook Chi; Julie B Zang; Aldo Mele; Robert B Darnell
Journal: Nature Date: 2009-06-17 Impact factor: 49.962

10. Ago-TNRC6 triggers microRNA-mediated decay by promoting two deadenylation steps.

Authors: Chyi-Ying A Chen; Dinghai Zheng; Zhenfang Xia; Ann-Bin Shyu
Journal: Nat Struct Mol Biol Date: 2009-10-18 Impact factor: 15.369

66 in total

Review 1. Post-transcriptional gene silencing, transcriptional gene silencing and human immunodeficiency virus.

Authors: Catalina Méndez; Chantelle L Ahlenstiel; Anthony D Kelleher
Journal: World J Virol Date: 2015-08-12

Review 2. Argonaute proteins: functional insights and emerging roles.

Authors: Gunter Meister
Journal: Nat Rev Genet Date: 2013-06-04 Impact factor: 53.242

3. Base-pairing probability in the microRNA stem region affects the binding and editing specificity of human A-to-I editing enzymes ADAR1-p110 and ADAR2.

Authors: Soh Ishiguro; Josephine Galipon; Rintaro Ishii; Yutaka Suzuki; Shinji Kondo; Mariko Okada-Hatakeyama; Masaru Tomita; Kumiko Ui-Tei
Journal: RNA Biol Date: 2018-07-24 Impact factor: 4.652

4. KSHV RNA-binding protein ORF57 inhibits P-body formation to promote viral multiplication by interaction with Ago2 and GW182.

Authors: Nishi R Sharma; Vladimir Majerciak; Michael J Kruhlak; Lulu Yu; Jeong Gu Kang; Acong Yang; Shuo Gu; Marvin J Fritzler; Zhi-Ming Zheng
Journal: Nucleic Acids Res Date: 2019-09-26 Impact factor: 16.971