Literature DB >> 12089286

Quantitative stability of DNA after extended storage of clinical specimens as determined by real-time PCR.

Keith R Jerome1, Meei-Li Huang, Anna Wald, Stacy Selke, Lawrence Corey.   

Abstract

Viral DNA stored for extended periods can be amplified by PCR. However, it is unknown whether stored specimens give accurate quantitative results by newer real-time PCR techniques. We therefore compared herpes simplex virus DNA levels in specimens before and after 16 months of storage. The levels of viral DNA remained stable whether the DNA was stored as purified DNA or unextracted DNA in a whole specimen.

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Year:  2002        PMID： 12089286      PMCID： PMC120597          DOI： 10.1128/JCM.40.7.2609-2611.2002

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

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Journal:  J Hepatol       Date:  1996-09       Impact factor: 25.083

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Journal:  N Engl J Med       Date:  1995-09-21       Impact factor: 91.245

6.  Storage conditions of blood samples and primer selection affect the yield of cDNA polymerase chain reaction products of hepatitis C virus.

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Journal:  J Clin Microbiol       Date:  1992-12       Impact factor: 5.948

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Authors:  T Schacker; J Zeh; H L Hu; E Hill; L Corey
Journal:  J Infect Dis       Date:  1998-12       Impact factor: 5.226

8.  Development of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.

Authors:  A J Ryncarz; J Goddard; A Wald; M L Huang; B Roizman; L Corey
Journal:  J Clin Microbiol       Date:  1999-06       Impact factor: 5.948
  8 in total
  78 in total

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Authors:  Alison L Drake; Alison C Roxby; Francisca Ongecha-Owuor; James Kiarie; Grace John-Stewart; Anna Wald; Barbra A Richardson; Jane Hitti; Julie Overbaugh; Sandra Emery; Carey Farquhar
Journal:  J Infect Dis       Date:  2011-12-06       Impact factor: 5.226

2.  Herpes diagnostic tests and their use.

Authors:  Nicholas J Van Wagoner; Edward W Hook
Journal:  Curr Infect Dis Rep       Date:  2012-04       Impact factor: 3.725

3.  Rapid polymerase chain reaction assay to detect herpes simplex virus in the genital tract of women in labor.

Authors:  Carolyn Gardella; Meei-Li Huang; Anna Wald; Amalia Magaret; Stacy Selke; Rhoda Morrow; Lawrence Corey
Journal:  Obstet Gynecol       Date:  2010-06       Impact factor: 7.661

Review 4.  Real-time PCR in clinical microbiology: applications for routine laboratory testing.

Authors:  M J Espy; J R Uhl; L M Sloan; S P Buckwalter; M F Jones; E A Vetter; J D C Yao; N L Wengenack; J E Rosenblatt; F R Cockerill; T F Smith
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

5.  Persistent genital herpes simplex virus-2 shedding years following the first clinical episode.

Authors:  Warren Phipps; Misty Saracino; Amalia Magaret; Stacy Selke; Mike Remington; Meei-Li Huang; Terri Warren; Corey Casper; Lawrence Corey; Anna Wald
Journal:  J Infect Dis       Date:  2010-12-09       Impact factor: 5.226

6.  Highly conserved intragenic HSV-2 sequences: Results from next-generation sequencing of HSV-2 UL and US regions from genital swabs collected from 3 continents.

Authors:  Christine Johnston; Amalia Magaret; Pavitra Roychoudhury; Alexander L Greninger; Anqi Cheng; Kurt Diem; Matthew P Fitzgibbon; Meei-Li Huang; Stacy Selke; Jairam R Lingappa; Connie Celum; Keith R Jerome; Anna Wald; David M Koelle
Journal:  Virology       Date:  2017-07-13       Impact factor: 3.616

7.  Prospective Characterization of the Risk Factors for Transmission and Symptoms of Primary Human Herpesvirus Infections Among Ugandan Infants.

Authors:  Soren Gantt; Jackson Orem; Elizabeth M Krantz; Rhoda Ashley Morrow; Stacy Selke; Meei-Li Huang; Joshua T Schiffer; Keith R Jerome; Annet Nakaganda; Anna Wald; Corey Casper; Lawrence Corey
Journal:  J Infect Dis       Date:  2016-02-24       Impact factor: 5.226

8.  Plasma and cerebrospinal fluid herpes simplex virus levels at diagnosis and outcome of neonatal infection.

Authors:  Ann J Melvin; Kathleen M Mohan; Joshua T Schiffer; Linda M Drolette; Amalia Magaret; Lawrence Corey; Anna Wald
Journal:  J Pediatr       Date:  2014-12-06       Impact factor: 4.406

9.  Virologic and immunologic evidence of multifocal genital herpes simplex virus 2 infection.

Authors:  Christine Johnston; Jia Zhu; Lichen Jing; Kerry J Laing; Christopher M McClurkan; Alexis Klock; Kurt Diem; Lei Jin; Jeffrey Stanaway; Elizabeth Tronstein; William W Kwok; Meei-Li Huang; Stacy Selke; Youyi Fong; Amalia Magaret; David M Koelle; Anna Wald; Lawrence Corey
Journal:  J Virol       Date:  2014-02-19       Impact factor: 5.103

10.  The Effect of Hormonal Contraception and Menstrual Cycle Timing on Genital Herpes Simplex Virus-2 Shedding and Lesions.

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Journal:  Sex Transm Dis       Date:  2019-01       Impact factor: 2.830
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