Literature DB >> 23027003

Intravital microscopy to image membrane trafficking in live rats.

Andrius Masedunskas1, Monika Sramkova, Laura Parente, Roberto Weigert.   

Abstract

Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.

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Year:  2013        PMID： 23027003      PMCID： PMC4052536          DOI： 10.1007/978-1-62703-056-4_9

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation.

Authors:  Warren R Zipfel; Rebecca M Williams; Richard Christie; Alexander Yu Nikitin; Bradley T Hyman; Watt W Webb
Journal:  Proc Natl Acad Sci U S A       Date:  2003-05-19       Impact factor: 11.205

2.  Functional studies of the kidney of living animals using multicolor two-photon microscopy.

Authors:  Kenneth W Dunn; Ruben M Sandoval; Katherine J Kelly; Pierre C Dagher; George A Tanner; Simon J Atkinson; Robert L Bacallao; Bruce A Molitoris
Journal:  Am J Physiol Cell Physiol       Date:  2002-09       Impact factor: 4.249

Review 3.  Intravital microscopy: a novel tool to study cell biology in living animals.

Authors:  Roberto Weigert; Monika Sramkova; Laura Parente; Panomwat Amornphimoltham; Andrius Masedunskas
Journal:  Histochem Cell Biol       Date:  2010-04-07       Impact factor: 4.304

4.  Multi-photon excitation microscopy in intact animals.

Authors:  Emily C Rothstein; Michael Nauman; Scott Chesnick; Robert S Balaban
Journal:  J Microsc       Date:  2006-04       Impact factor: 1.758

5.  Expression of plasmid DNA in the salivary gland epithelium: novel approaches to study dynamic cellular processes in live animals.

Authors:  Monika Sramkova; Andrius Masedunskas; Laura Parente; Alfredo Molinolo; Roberto Weigert
Journal:  Am J Physiol Cell Physiol       Date:  2009-09-30       Impact factor: 4.249

6.  Role for the actomyosin complex in regulated exocytosis revealed by intravital microscopy.

Authors:  Andrius Masedunskas; Monika Sramkova; Laura Parente; Katiuchia Uzzun Sales; Panomwat Amornphimoltham; Thomas H Bugge; Roberto Weigert
Journal:  Proc Natl Acad Sci U S A       Date:  2011-08-01       Impact factor: 11.205

Review 7.  Intravital microscopy as a tool to study drug delivery in preclinical studies.

Authors:  Panomwat Amornphimoltham; Andrius Masedunskas; Roberto Weigert
Journal:  Adv Drug Deliv Rev       Date:  2010-10-07       Impact factor: 15.470

8.  Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy.

Authors:  Ruben M Sandoval; Michael D Kennedy; Philip S Low; Bruce A Molitoris
Journal:  Am J Physiol Cell Physiol       Date:  2004-04-21       Impact factor: 4.249

9.  Degranulation of rat salivary glands following treatment with receptor-selective agonists.

Authors:  B Peter; M A Van Waarde; A Vissink; E J 's-Gravenmade; A W Konings
Journal:  Clin Exp Pharmacol Physiol       Date:  1995-05       Impact factor: 2.557

10.  Intravital two-photon microscopy for studying the uptake and trafficking of fluorescently conjugated molecules in live rodents.

Authors:  Andrius Masedunskas; Roberto Weigert
Journal:  Traffic       Date:  2008-07-18       Impact factor: 6.215
  10 in total
  14 in total

1.  Intravital microscopy for imaging subcellular structures in live mice expressing fluorescent proteins.

Authors:  Andrius Masedunskas; Natalie Porat-Shliom; Muhibullah Tora; Oleg Milberg; Roberto Weigert
Journal:  J Vis Exp       Date:  2013-09-01       Impact factor: 1.355

2.  Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes by Multiphoton Confocal Microscopy.

Authors:  Billur Akkaya; Olena Kamenyeva; Juraj Kabat; Ryan Kissinger
Journal:  Methods Mol Biol       Date:  2021

Review 3.  Imaging the dynamics of endocytosis in live mammalian tissues.

Authors:  Roberto Weigert
Journal:  Cold Spring Harb Perspect Biol       Date:  2014-04-01       Impact factor: 10.005

4.  Axial beam scanning in multiphoton microscopy with MEMS-based actuator.

Authors:  Xiyu Duan; Haijun Li; Xue Li; Kenn R Oldham; Thomas D Wang
Journal:  Opt Express       Date:  2017-02-06       Impact factor: 3.894

5.  In vivo tissue-wide synchronization of mitochondrial metabolic oscillations.

Authors:  Natalie Porat-Shliom; Yun Chen; Muhibullah Tora; Akiko Shitara; Andrius Masedunskas; Roberto Weigert
Journal:  Cell Rep       Date:  2014-10-16       Impact factor: 9.423

6.  MEMS-based multiphoton endomicroscope for repetitive imaging of mouse colon.

Authors:  Xiyu Duan; Haijun Li; Zhen Qiu; Bishnu P Joshi; Asha Pant; Arlene Smith; Katsuo Kurabayashi; Kenn R Oldham; Thomas D Wang
Journal:  Biomed Opt Express       Date:  2015-07-29       Impact factor: 3.732

7.  Probing the role of the actin cytoskeleton during regulated exocytosis by intravital microscopy.

Authors:  Oleg Milberg; Muhibullah Tora; Akiko Shitara; Taishin Takuma; Andrius Masedunskas; Roberto Weigert
Journal:  Methods Mol Biol       Date:  2014

Review 8.  Imaging cell biology in live animals: ready for prime time.

Authors:  Roberto Weigert; Natalie Porat-Shliom; Panomwat Amornphimoltham
Journal:  J Cell Biol       Date:  2013-06-24       Impact factor: 10.539

9.  Intravital Microscopy Reveals Differences in the Kinetics of Endocytic Pathways between Cell Cultures and Live Animals.

Authors:  Andrius Masedunskas; Natalie Porat-Shliom; Kamil Rechache; Myo-Pale' Aye; Roberto Weigert
Journal:  Cells       Date:  2012-11-16       Impact factor: 6.600

10.  Polyethylenimine-mediated expression of transgenes in the acinar cells of rats salivary glands in vivo.

Authors:  Monika Sramkova; Laura Parente; Timothy Wigand; Myo-Pale' Aye; Akiko Shitara; Roberto Weigert
Journal:  Front Cell Dev Biol       Date:  2015-01-09
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