Stefany D Primeaux1, H Douglas Braymer, George A Bray. 1. Joint Diabetes, Endocrinology and Metabolism Program, Louisiana State University System, Louisiana State University Health Science Center-New Orleans, New Orleans, LA 70112, USA. sprime@lsuhsc.edu
Abstract
BACKGROUND: The gastrointestinal tract (GI) is important for detection and transport of consumed nutrients and has been implicated in susceptibility to diet-induced obesity in various rat strains. AIMS: The current studies investigated the regulation of CD36, a receptor which facilitates uptake of long-chain fatty acids, in the GI tract of obesity-prone Osborne-Mendel and obesity-resistant S5B rats fed a high-fat diet. METHODS: Osborne-Mendel and S5B rats consumed a high-fat diet (HFD, 55 % kcal from fat) or a low-fat diet (10 % kcal from fat) for either 3 or 14 days. CD36 messenger RNA (mRNA) levels were measured from circumvallate papillae of the tongue and from duodenal enterocytes. RESULTS: In Osborne-Mendel rats, consumption of HFD for 3 and 14 days led to an increase in CD36 mRNA on circumvallate papillae and in duodenal enterocytes. CD36 mRNA levels were positively correlated with body weight gain and kilocalories consumed at 3 days. In S5B rats, consumption of HFD for 3 days did not alter CD36 mRNA levels on circumvallate papillae or in the duodenum. Duodenal CD36 levels were elevated in S5B rats following 14 days of HFD consumption. CD36 mRNA levels in the duodenum were positively correlated with body weight gain and kilocalories consumed at 14 days. CONCLUSIONS: These data support the differential sensing of nutrients by two regions of the GI tract of obesity-prone and obesity-resistant rats consuming HFD and suggest a role for CD36 in the strain-specific susceptibility to obesity.
BACKGROUND: The gastrointestinal tract (GI) is important for detection and transport of consumed nutrients and has been implicated in susceptibility to diet-induced obesity in various rat strains. AIMS: The current studies investigated the regulation of CD36, a receptor which facilitates uptake of long-chain fatty acids, in the GI tract of obesity-prone Osborne-Mendel and obesity-resistant S5B rats fed a high-fat diet. METHODS: Osborne-Mendel and S5B rats consumed a high-fat diet (HFD, 55 % kcal from fat) or a low-fat diet (10 % kcal from fat) for either 3 or 14 days. CD36 messenger RNA (mRNA) levels were measured from circumvallate papillae of the tongue and from duodenal enterocytes. RESULTS: In Osborne-Mendel rats, consumption of HFD for 3 and 14 days led to an increase in CD36 mRNA on circumvallate papillae and in duodenal enterocytes. CD36 mRNA levels were positively correlated with body weight gain and kilocalories consumed at 3 days. In S5B rats, consumption of HFD for 3 days did not alter CD36 mRNA levels on circumvallate papillae or in the duodenum. Duodenal CD36 levels were elevated in S5B rats following 14 days of HFD consumption. CD36 mRNA levels in the duodenum were positively correlated with body weight gain and kilocalories consumed at 14 days. CONCLUSIONS: These data support the differential sensing of nutrients by two regions of the GI tract of obesity-prone and obesity-resistant rats consuming HFD and suggest a role for CD36 in the strain-specific susceptibility to obesity.
Authors: Christina S-Y Chen; Elias M Bench; Timothy D Allerton; Allyson L Schreiber; Kenneth P Arceneaux; Stefany D Primeaux Journal: Am J Physiol Regul Integr Comp Physiol Date: 2013-10-23 Impact factor: 3.619
Authors: Stefany D Primeaux; Taniya de Silva; Tony H Tzeng; Monica C Chiang; Daniel S Hsia Journal: Rev Endocr Metab Disord Date: 2016-06 Impact factor: 6.514
Authors: Michal Pravenec; Laura M Saba; Václav Zídek; Vladimír Landa; Petr Mlejnek; Jan Šilhavý; Miroslava Šimáková; Hynek Strnad; Jaroslava Trnovská; Vojtěch Škop; Martina Hüttl; Irena Marková; Olena Oliyarnyk; Hana Malínská; Ludmila Kazdová; Harry Smith; Boris Tabakoff Journal: Physiol Genomics Date: 2017-11-10 Impact factor: 3.107
Authors: Jonquil M Poret; Claire Battle; Alan J Mouton; Darryl A Gaudet; Flavia Souza-Smith; Jason D Gardner; H Douglas Braymer; Lisa Harrison-Bernard; Stefany D Primeaux Journal: Life Sci Date: 2019-03-11 Impact factor: 5.037
Authors: Sophie Kindleysides; Kathryn L Beck; Daniel C I Walsh; Lisa Henderson; Shakeela N Jayasinghe; Matt Golding; Bernhard H Breier Journal: Nutrients Date: 2017-08-15 Impact factor: 5.717
Authors: J M Poret; F Souza-Smith; S J Marcell; D A Gaudet; T H Tzeng; H D Braymer; L M Harrison-Bernard; S D Primeaux Journal: Int J Obes (Lond) Date: 2017-11-20 Impact factor: 5.095