OBJECTIVE: This study was designed to investigate whether dietary fat and genetic background might differentially alter the expression of hypothalamic genes involved in food intake. RESEARCH METHODS AND PROCEDURES: Three-month-old Osborne-Mendel (OM) and S5B/Pl rats were fed either a high-fat or a low-fat diet for 14 days. mRNA for neuropeptide Y (NPY), corticotrophin-releasing hormone, NPY Y-1 receptor and Y-5 receptor, and serotonin 2c (5-HT2c) receptors were measured using Northern blotting or ribonuclease protection assays. RESULTS: OM rats showed an increased expression of NPY and corticotrophin-releasing hormone compared with S5B/Pl rats. The expression of NPY-Y1 and -Y5 receptor mRNA was significantly higher in the hypothalamus of OM rats compared with S5B/Pl rats. The expression of 5HT-2c receptor mRNA was significantly reduced in both strains of rats eating a high-fat diet when compared with the animals eating the low-fat diet. DISCUSSION: These data suggest that over activity of the NPY system may contribute to the development of obesity in OM rats and that expression of the 5HT-2c receptor gene may be modulated by dietary fat.
OBJECTIVE: This study was designed to investigate whether dietary fat and genetic background might differentially alter the expression of hypothalamic genes involved in food intake. RESEARCH METHODS AND PROCEDURES: Three-month-old Osborne-Mendel (OM) and S5B/Pl rats were fed either a high-fat or a low-fat diet for 14 days. mRNA for neuropeptide Y (NPY), corticotrophin-releasing hormone, NPY Y-1 receptor and Y-5 receptor, and serotonin 2c (5-HT2c) receptors were measured using Northern blotting or ribonuclease protection assays. RESULTS: OM rats showed an increased expression of NPY and corticotrophin-releasing hormone compared with S5B/Pl rats. The expression of NPY-Y1 and -Y5 receptor mRNA was significantly higher in the hypothalamus of OM rats compared with S5B/Pl rats. The expression of 5HT-2c receptor mRNA was significantly reduced in both strains of rats eating a high-fat diet when compared with the animals eating the low-fat diet. DISCUSSION: These data suggest that over activity of the NPY system may contribute to the development of obesity in OM rats and that expression of the 5HT-2c receptor gene may be modulated by dietary fat.
Authors: Christina S-Y Chen; Elias M Bench; Timothy D Allerton; Allyson L Schreiber; Kenneth P Arceneaux; Stefany D Primeaux Journal: Am J Physiol Regul Integr Comp Physiol Date: 2013-10-23 Impact factor: 3.619
Authors: Regina L H Watanabe; Iracema S Andrade; Mônica M Telles; Kelse T Albuquerque; Cláudia M O Nascimento; Lila M Oyama; Dulce E Casarini; Eliane B Ribeiro Journal: Cell Mol Neurobiol Date: 2010-06-05 Impact factor: 5.046
Authors: Panayotis K Thanos; Ronald Kim; Jacob Cho; Michael Michaelides; Brenda J Anderson; Stefany D Primeaux; George A Bray; Gene-Jack Wang; John K Robinson; Nora D Volkow Journal: Physiol Behav Date: 2010-08-27