Literature DB >> 22691798

'Seeding' with protease to optimize protein crystallization conditions in in situ proteolysis.

Jinguang Huang1, Yanmei Gong, Dan Huang, Lesley Haire, Junfeng Liu, Youliang Peng.   

Abstract

In situ proteolysis is one of the most effective rescue strategies for protein crystallization, and optimization of the ratio between the protein and the protease is one of the key steps in the process. Seeding is a very powerful tool to optimize crystallization conditions and can be performed by most crystallization robots. Addition of protease instead of seed stock using a robot can be used to optimize the concentration of protease in in situ proteolysis experiments and has been successfully tested using two proteins.

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Year:  2012        PMID： 22691798      PMCID： PMC3374523          DOI： 10.1107/S174430911201161X

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

Review 1.  Seeds to crystals.

Authors:  Terese Bergfors
Journal:  J Struct Biol       Date:  2003-04       Impact factor: 2.867

Review 2.  The protein as a variable in protein crystallization.

Authors:  Glenn E Dale; Christian Oefner; Allan D'Arcy
Journal:  J Struct Biol       Date:  2003-04       Impact factor: 2.867

Review 3.  The use of recombinant methods and molecular engineering in protein crystallization.

Authors:  Zygmunt S Derewenda
Journal:  Methods       Date:  2004-11       Impact factor: 3.608

4.  Preliminary X-ray analysis of a human V(H) fragment at 1.8 A resolution.

Authors:  Rajneesh Kumar Gaur; Michael B Kupper; Rainer Fischer; Kurt M V Hoffmann
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2004-04-21

Review 5.  Structural genomics: keeping up with expanding knowledge of the protein universe.

Authors:  Marek Grabowski; Andrzej Joachimiak; Zbyszek Otwinowski; Wladek Minor
Journal:  Curr Opin Struct Biol       Date:  2007-06-22       Impact factor: 6.809

6.  An automated microseed matrix-screening method for protein crystallization.

Authors:  Allan D'Arcy; Frederic Villard; May Marsh
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2007-03-16

7.  Chemical screening methods to identify ligands that promote protein stability, protein crystallization, and structure determination.

Authors:  Masoud Vedadi; Frank H Niesen; Abdellah Allali-Hassani; Oleg Y Fedorov; Patrick J Finerty; Gregory A Wasney; Ron Yeung; Cheryl Arrowsmith; Linda J Ball; Helena Berglund; Raymond Hui; Brian D Marsden; Pär Nordlund; Michael Sundstrom; Johan Weigelt; Aled M Edwards
Journal:  Proc Natl Acad Sci U S A       Date:  2006-10-11       Impact factor: 11.205

8.  Replication protein A. Characterization and crystallization of the DNA binding domain.

Authors:  R A Pfuetzner; A Bochkarev; L Frappier; A M Edwards
Journal:  J Biol Chem       Date:  1997-01-03       Impact factor: 5.157

9.  In situ proteolysis for protein crystallization and structure determination.

Authors:  Aiping Dong; Xiaohui Xu; Aled M Edwards; Changsoo Chang; Maksymilian Chruszcz; Marianne Cuff; Marcin Cymborowski; Rosa Di Leo; Olga Egorova; Elena Evdokimova; Ekaterina Filippova; Jun Gu; Jennifer Guthrie; Alexandr Ignatchenko; Andrzej Joachimiak; Natalie Klostermann; Youngchang Kim; Yuri Korniyenko; Wladek Minor; Qiuni Que; Alexei Savchenko; Tatiana Skarina; Kemin Tan; Alexander Yakunin; Adelinda Yee; Veronica Yim; Rongguang Zhang; Hong Zheng; Masato Akutsu; Cheryl Arrowsmith; George V Avvakumov; Alexey Bochkarev; Lars-Göran Dahlgren; Sirano Dhe-Paganon; Slav Dimov; Ludmila Dombrovski; Patrick Finerty; Susanne Flodin; Alex Flores; Susanne Gräslund; Martin Hammerström; Maria Dolores Herman; Bum-Soo Hong; Raymond Hui; Ida Johansson; Yongson Liu; Martina Nilsson; Lyudmila Nedyalkova; Pär Nordlund; Tomas Nyman; Jinrong Min; Hui Ouyang; Hee-won Park; Chao Qi; Wael Rabeh; Limin Shen; Yang Shen; Deepthi Sukumard; Wolfram Tempel; Yufeng Tong; Lionel Tresagues; Masoud Vedadi; John R Walker; Johan Weigelt; Martin Welin; Hong Wu; Ting Xiao; Hong Zeng; Haizhong Zhu
Journal:  Nat Methods       Date:  2007-11-04       Impact factor: 28.547

10.  In situ proteolysis to generate crystals for structure determination: an update.

Authors:  Amy Wernimont; Aled Edwards
Journal:  PLoS One       Date:  2009-04-07       Impact factor: 3.240
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  1 in total

Review 1.  High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur.

Authors:  Patrick Weber; Cédric Pissis; Rafael Navaza; Ariel E Mechaly; Frederick Saul; Pedro M Alzari; Ahmed Haouz
Journal:  Molecules       Date:  2019-12-05       Impact factor: 4.411
  1 in total

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