Literature DB >> 2245476

The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

F Gruber1, J Visser, C P Kubicek, L H de Graaff.   

Abstract

Six uridine auxotroph mutants of Trichoderma reesei QM 9414 were isolated by resistance to 5-fluoroorotic acid and one strain was identified as OMP-decarboxylase negative (pyr-) by a radiometric enzyme assay. Transformation to uridine prototrophy was achieved with the pyr4 gene of Neurospora crassa (up to 1500 transformants/micrograms) and with pyrA of Aspergillus niger (700-800 transformants/micrograms). In many transformants the PYR+ function seems to be present as extrachromosomal DNA. There is evidence for a correlation between the stability of transformants and integration of the vector in the genome whereas unstable transformants are obtained when autonomous replication of the plasmid occurs.

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Year:  1990        PMID： 2245476     DOI： 10.1007/bf00321118

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  14 in total

Review 1.  Recombinant DNA in filamentous fungi: progress and prospects.

Authors:  J Rambosek; J Leach
Journal:  Crit Rev Biotechnol       Date:  1987       Impact factor: 8.429

2.  Applications of cellulases.

Authors:  M Mandels
Journal:  Biochem Soc Trans       Date:  1985-04       Impact factor: 5.407

3.  Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.

Authors:  M J Hynes; C M Corrick; J A King
Journal:  Mol Cell Biol       Date:  1983-08       Impact factor: 4.272

4.  Tryptophan auxotrophic mutants in Aspergillus niger: inactivation of the trpC gene by cotransformation mutagenesis.

Authors:  T Goosen; F van Engelenburg; F Debets; K Swart; K Bos; H van den Broek
Journal:  Mol Gen Genet       Date:  1989-10

5.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

6.  beta-Glucosidase excretion by Trichoderma pseudokoningii: correlation with cell wall bound beta-1.3-glucanase activities.

Authors:  C P Kubicek
Journal:  Arch Microbiol       Date:  1982-10       Impact factor: 2.552

7.  Isolation and transformation of the pyruvate kinase gene of Aspergillus nidulans.

Authors:  L de Graaff; H van den Broek; J Visser
Journal:  Curr Genet       Date:  1988-04       Impact factor: 3.886

8.  Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.

Authors:  F P Buxton; A Radford
Journal:  Mol Gen Genet       Date:  1983

9.  Presence and regulation of the alpha-ketoglutarate dehydrogenase multienzyme complex in the filamentous fungus Aspergillus niger.

Authors:  B Meixner-Monori; C P Kubicek; A Habison; E M Kubicek-Pranz; M Röhr
Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490

10.  A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei.

Authors:  M Penttilä; H Nevalainen; M Rättö; E Salminen; J Knowles
Journal:  Gene       Date:  1987       Impact factor: 3.688
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  115 in total

1.  Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum.

Authors:  Zhonghai Li; Guangshan Yao; Ruimei Wu; Liwei Gao; Qinbiao Kan; Meng Liu; Piao Yang; Guodong Liu; Yuqi Qin; Xin Song; Yaohua Zhong; Xu Fang; Yinbo Qu
Journal:  PLoS Genet       Date:  2015-09-11       Impact factor: 5.917

2.  Roles of protein kinase A and adenylate cyclase in light-modulated cellulase regulation in Trichoderma reesei.

Authors:  André Schuster; Doris Tisch; Verena Seidl-Seiboth; Christian P Kubicek; Monika Schmoll
Journal:  Appl Environ Microbiol       Date:  2012-01-27       Impact factor: 4.792

3.  Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

Authors:  J L Smith; F T Bayliss; M Ward
Journal:  Curr Genet       Date:  1991-01       Impact factor: 3.886

4.  Transcriptional regulation of xyr1, encoding the main regulator of the xylanolytic and cellulolytic enzyme system in Hypocrea jecorina.

Authors:  Astrid R Mach-Aigner; Marion E Pucher; Matthias G Steiger; Gudrun E Bauer; Sonja J Preis; Robert L Mach
Journal:  Appl Environ Microbiol       Date:  2008-09-12       Impact factor: 4.792

5.  Glucose-induced secretion of Trichoderma reesei xylanases.

Authors:  W Kurzatkowski; A Törrönen; J Filipek; R L Mach; P Herzog; S Sowka; C P Kubicek
Journal:  Appl Environ Microbiol       Date:  1996-08       Impact factor: 4.792

6.  Signal transduction by Tga3, a novel G protein alpha subunit of Trichoderma atroviride.

Authors:  Susanne Zeilinger; Barbara Reithner; Valeria Scala; Isabel Peissl; Matteo Lorito; Robert L Mach
Journal:  Appl Environ Microbiol       Date:  2005-03       Impact factor: 4.792

7.  Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.

Authors:  V Campuzano; P del Valle; J I de Vicente; A P Eslava; M I Alvarez
Journal:  Curr Genet       Date:  1993-12       Impact factor: 3.886

8.  Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei.

Authors:  S P Goller; D Schoisswohl; M Baron; M Parriche; C P Kubicek
Journal:  Appl Environ Microbiol       Date:  1998-09       Impact factor: 4.792

9.  D-xylose metabolism in Hypocrea jecorina: loss of the xylitol dehydrogenase step can be partially compensated for by lad1-encoded L-arabinitol-4-dehydrogenase.

Authors:  Bernhard Seiboth; Lukas Hartl; Manuela Pail; Christian P Kubicek
Journal:  Eukaryot Cell       Date:  2003-10

10.  Light-dependent roles of the G-protein alpha subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei).

Authors:  Christian Seibel; Gabriela Gremel; Roberto do Nascimento Silva; André Schuster; Christian P Kubicek; Monika Schmoll
Journal:  BMC Biol       Date:  2009-09-03       Impact factor: 7.431
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