Literature DB >> 8299173

Isolation, characterization and mapping of pyrimidine auxotrophs of Phycomyces blakesleeanus.

V Campuzano1, P del Valle, J I de Vicente, A P Eslava, M I Alvarez.   

Abstract

A total of seven pyrimidine auxotrophs of Phycomyces were isolated from among 5-fluoroorotate acid (5-FOA)-resistant mutants. They were classified by complementation into two groups. A representative mutant strain belonging to one group was deficient in orotate phosphoribosyl transferase (OPRTase; EC 2.4.2.10) activity; the mutant strain belonging to the second group was deficient in orotidine-5'-monophosphate decarboxylase (OMPdecase; EC 4.1.1.23). These mutants are defective in the genes pyrF and pyrG respectively. The results from random spore analysis, tetrad analysis, and gene-centromere distances showed that these two markers are located in linkage group VI, with pyrG being a proximal marker and pyrF a distal one.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8299173     DOI： 10.1007/BF00351715

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  27 in total

1.  The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

Authors:  F Gruber; J Visser; C P Kubicek; L H de Graaff
Journal:  Curr Genet       Date:  1990-07       Impact factor: 3.886

2.  Positive screening and transformation of ura5 mutants in the fungus Podospora anserina: characterization of the transformants.

Authors:  V Razanamparany; J Bégueret
Journal:  Curr Genet       Date:  1986       Impact factor: 3.886

3.  Mutants of Phycomyces with abnormal phototropism.

Authors:  K Bergman; A P Eslava; E Cerdá-Olmedo
Journal:  Mol Gen Genet       Date:  1973

4.  Orotate phosphoribosyltransferase: orotidylate decarboxylase (erythrocyte).

Authors:  R F Silva; D Hatfield
Journal:  Methods Enzymol       Date:  1978       Impact factor: 1.600

5.  Cloning, mapping and molecular analysis of the pyrG (orotidine-5'-phosphate decarboxylase) gene of Aspergillus nidulans.

Authors:  B R Oakley; J E Rinehart; B L Mitchell; C E Oakley; C Carmona; G L Gray; G S May
Journal:  Gene       Date:  1987       Impact factor: 3.688

6.  Recombination between mutants of Phycomyces with abnormal phototropism.

Authors:  A P Eslava; M I Alvarez; E D Lipson; D Presti; K Kong
Journal:  Mol Gen Genet       Date:  1976-09-23

7.  Development of a homologous transformation system for Aspergillus niger based on the pyrG gene.

Authors:  W van Hartingsveldt; I E Mattern; C M van Zeijl; P H Pouwels; C A van den Hondel
Journal:  Mol Gen Genet       Date:  1987-01

8.  Isolation and transformation of uracil auxotrophs of the lignin-degrading basidiomycete Phanerochaete chrysosporium.

Authors:  L Akileswaran; M Alic; E K Clark; J L Hornick; M H Gold
Journal:  Curr Genet       Date:  1993       Impact factor: 3.886

9.  A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae.

Authors:  Y M de Ruiter-Jacobs; M Broekhuijsen; S E Unkles; E I Campbell; J R Kinghorn; R Contreras; P H Pouwels; C A van den Hondel
Journal:  Curr Genet       Date:  1989-09       Impact factor: 3.886

10.  Mutations affecting sexual development in Phycomyces blakesleeanus.

Authors:  R P Sutter
Journal:  Proc Natl Acad Sci U S A       Date:  1975-01       Impact factor: 11.205
View more
  6 in total

1.  Two origins for the gene encoding alpha-isopropylmalate synthase in fungi.

Authors:  Erica M Larson; Alexander Idnurm
Journal:  PLoS One       Date:  2010-07-15       Impact factor: 3.240

2.  Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar.

Authors:  H Horiuchi; N Takaya; K Yanai; M Nakamura; A Ohta; M Takagi
Journal:  Curr Genet       Date:  1995-04       Impact factor: 3.886

3.  Isolation, characterization and transformation, by autonomous replication, of Mucor circinelloides OMPdecase-deficient mutants.

Authors:  E P Benito; V Campuzano; M A Lŏpez-Matas; J I De Vicente; A P Eslava
Journal:  Mol Gen Genet       Date:  1995-07-28

4.  Isolation and characterization of phototropism mutants of Phycomyces insensitive to ultraviolet light.

Authors:  V Campuzano; P Galland; H Senger; M I Alvarez; A P Eslava
Journal:  Curr Genet       Date:  1994-07       Impact factor: 3.886

5.  The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent.

Authors:  Maria Villarino; Antonieta De Cal; Paloma Melgarejo; Inmaculada Larena; Eduardo A Espeso
Journal:  Microb Biotechnol       Date:  2015-10-15       Impact factor: 5.813

6.  A new genetic linkage map of the zygomycete fungus Phycomyces blakesleeanus.

Authors:  Suman Chaudhary; Silvia Polaino; Viplendra P S Shakya; Alexander Idnurm
Journal:  PLoS One       Date:  2013-03-14       Impact factor: 3.240
  6 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.