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Literature DB >> 6308396

Cloning of the structural gene for orotidine 5'-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli.

Abstract

A Neurospora gene bank in plasmid pRK9 was used to complement pyrimidine auxotrophs in E. coli. Two plasmids were obtained that complement a pyrF mutant of E. coli. These plasmids hybridise to Neurospora DNA and transform a pyr-4 strain of Neurospora. The promoter used in expressing the orotidine 5'-monophosphate carboxylase in E. coli is within the Neurospora sequence.

Entities: Chemical Species

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Year: 1983 PMID： 6308396 DOI： 10.1007/bf00331067

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

11 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. A possible model for the structure of the Neurospora carbamoyl phosphate synthase-aspartate carbamoyl transferase complex enzyme.

Authors: A J Makoff; F P Buxton; A Radford
Journal: Mol Gen Genet Date: 1978-05-31

3. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

4. Isolation of spheroplast and membrane vesicles from yeast and filamentous fungi.

Authors: W R Wiley
Journal: Methods Enzymol Date: 1974 Impact factor: 1.600

5. Rapid and efficient cosmid cloning.

Authors: D Ish-Horowicz; J F Burke
Journal: Nucleic Acids Res Date: 1981-07-10 Impact factor: 16.971

6. Evidence for transcriptional regulation of orotidine-5'-phosphate decarboxylase in yeast by hybridization of mRNA to the yeast structural gene cloned in Escherichia coli.

Authors: M L Bach; F Lacroute; D Botstein
Journal: Proc Natl Acad Sci U S A Date: 1979-01 Impact factor: 11.205

7. Efficient transformation of Neurospora crassa by utilizing hybrid plasmid DNA.

Authors: M E Case; M Schweizer; S R Kushner; N H Giles
Journal: Proc Natl Acad Sci U S A Date: 1979-10 Impact factor: 11.205

8. Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassa.

Authors: D Vapnek; J A Hautala; J W Jacobson; N H Giles; S R Kushner
Journal: Proc Natl Acad Sci U S A Date: 1977-08 Impact factor: 11.205

9. Glutamine utilization in both the arginine-specific and pyrimidine-specific carbamoyl phosphate synthase enzymes of eurospora crassa.

Authors: A J Makoff; A Radford
Journal: Mol Gen Genet Date: 1976-12-08

10. 5'-Untranslated sequences of two structural genes in the qa gene cluster of Neurospora crassa.

Authors: N K Alton; F Buxton; V Patel; N H Giles; D Vapnek
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

29 in total

1. The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

Authors: F Gruber; J Visser; C P Kubicek; L H de Graaff
Journal: Curr Genet Date: 1990-07 Impact factor: 3.886

2. Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

Authors: J L Smith; F T Bayliss; M Ward
Journal: Curr Genet Date: 1991-01 Impact factor: 3.886

3. Cloning and preliminary characterization of a molybdenum cofactor gene of Neurospora crassa.

Authors: N Stuart Dunn-Coleman
Journal: Curr Genet Date: 1984-10 Impact factor: 3.886

4. A new, rapid and efficient transformation procedure for Neurospora.

Authors: S S Dhawale; J V Paietta; G A Marzluf
Journal: Curr Genet Date: 1984-01 Impact factor: 3.886

5. Regulation of Aspergillus nidulans penicillin biosynthesis and penicillin biosynthesis genes acvA and ipnA by glucose.

Authors: A A Brakhage; P Browne; G Turner
Journal: J Bacteriol Date: 1992-06 Impact factor: 3.490

6. The product of the het-C heterokaryon incompatibility gene of Neurospora crassa has characteristics of a glycine-rich cell wall protein.

Authors: S J Saupe; G A Kuldau; M L Smith; N L Glass
Journal: Genetics Date: 1996-08 Impact factor: 4.562

10. Suppressor mutants of Neurospora crassa that tolerate allelic differences at single or at multiple heterokaryon incompatibility loci.

Authors: M T Arganoza; J Ohrnberger; J Min; R A Akins
Journal: Genetics Date: 1994-07 Impact factor: 4.562