Literature DB >> 3127274

A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei.

M Penttilä1, H Nevalainen, M Rättö, E Salminen, J Knowles.   

Abstract

An efficient transformation system for the cellulolytic filamentous fungus Trichoderma reesei has been developed. Transformation was obtained with plasmid carrying the dominant selectable marker amdS or the argB gene of Aspergillus nidulans, which was found to complement the respective argB mutation of T. reesei. The transformation frequency can be up to 600 transformants per microgram of transforming DNA. The efficiency of co-transformation with unselected DNA was high (approx. 80%). The transforming DNA was found to be integrated at several different locations, often in multiple tandem copies in the T. reesei genome. In addition, the Escherichia coli beta-galactosidase was expressed in T. reesei in enzymatically active form from the A. nidulans gpd promoter.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3127274     DOI： 10.1016/0378-1119(87)90110-7

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  178 in total

1.  Cotransformation of Trichoderma harzianum with beta-glucuronidase and green fluorescent protein genes provides a useful tool for monitoring fungal growth and activity in natural soils.

Authors:  Y S Bae; G R Knudsen
Journal:  Appl Environ Microbiol       Date:  2000-02       Impact factor: 4.792

2.  Surface adhesion of fusion proteins containing the hydrophobins HFBI and HFBII from Trichoderma reesei.

Authors:  Markus Linder; Geza R Szilvay; Tiina Nakari-Setälä; Hans Söderlund; Merja Penttilä
Journal:  Protein Sci       Date:  2002-09       Impact factor: 6.725

3.  The development of a heterologous transformation system for the cellulolytic fungus Trichoderma reesei based on a pyrG-negative mutant strain.

Authors:  F Gruber; J Visser; C P Kubicek; L H de Graaff
Journal:  Curr Genet       Date:  1990-07       Impact factor: 3.886

4.  Synergistic and Dose-Controlled Regulation of Cellulase Gene Expression in Penicillium oxalicum.

Authors:  Zhonghai Li; Guangshan Yao; Ruimei Wu; Liwei Gao; Qinbiao Kan; Meng Liu; Piao Yang; Guodong Liu; Yuqi Qin; Xin Song; Yaohua Zhong; Xu Fang; Yinbo Qu
Journal:  PLoS Genet       Date:  2015-09-11       Impact factor: 5.917

5.  Sequence of the cloned pyr4 gene of Trichoderma reesei and its use as a homologous selectable marker for transformation.

Authors:  J L Smith; F T Bayliss; M Ward
Journal:  Curr Genet       Date:  1991-01       Impact factor: 3.886

6.  Secretome of the Coprophilous Fungus Doratomyces stemonitis C8, Isolated from Koala Feces.

Authors:  Robyn Peterson; Jasmine Grinyer; Helena Nevalainen
Journal:  Appl Environ Microbiol       Date:  2011-04-15       Impact factor: 4.792

7.  Enhancing Cellulase and Hemicellulase Production in Trichoderma orientalis EU7-22 via Knockout of the creA.

Authors:  Chuannan Long; Yijin Cheng; Jingjing Cui; Jian Liu; Lihui Gan; Bin Zeng; Minnan Long
Journal:  Mol Biotechnol       Date:  2018-01       Impact factor: 2.695

8.  Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae.

Authors:  V V Joutsjoki
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

9.  The ire1 and ptc2 genes involved in the unfolded protein response pathway in the filamentous fungus Trichoderma reesei.

Authors:  M Valkonen; M Penttilä; M Saloheimo
Journal:  Mol Genet Genomics       Date:  2004-10-08       Impact factor: 3.291

10.  Establishment of an efficient RNA silencing system in Trichoderma koningii using DsRed as a reporter.

Authors:  Shaowen Wang; Miao Xing; Shenli Tian; Deming Gou; Baiqu Huang; Gang Liu
Journal:  Folia Microbiol (Praha)       Date:  2013-04-16       Impact factor: 2.099
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.