Literature DB >> 22450983

Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

Liang Chen1, Kalyan D Chavda, José R Mediavilla, Yanan Zhao, Henry S Fraimow, Stephen G Jenkins, Michael H Levi, Tao Hong, Albert D Rojtman, Christine C Ginocchio, Robert A Bonomo, Barry N Kreiswirth.   

Abstract

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

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Year:  2012        PMID： 22450983      PMCID： PMC3370784          DOI： 10.1128/AAC.00316-12

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  15 in total

Review 1.  Alarming β-lactamase-mediated resistance in multidrug-resistant Enterobacteriaceae.

Authors:  Karen Bush
Journal:  Curr Opin Microbiol       Date:  2010-10-01       Impact factor: 7.934

2.  Evaluation of PCR-based testing for surveillance of KPC-producing carbapenem-resistant members of the Enterobacteriaceae family.

Authors:  Vered Schechner; Keren Straus-Robinson; David Schwartz; Iris Pfeffer; Jalal Tarabeia; Rina Moskovich; Inna Chmelnitsky; Mitchell J Schwaber; Yehuda Carmeli; Shiri Navon-Venezia
Journal:  J Clin Microbiol       Date:  2009-08-12       Impact factor: 5.948

3.  Rapid detection of blaKPC carbapenemase genes by internally controlled real-time PCR assay using bactec blood culture bottles.

Authors:  Musa Hindiyeh; Gill Smollan; Zehava Grossman; Daniela Ram; Jana Robinov; Natasha Belausov; Debbie Ben-David; Ilana Tal; Yehudit Davidson; Ari Shamiss; Ella Mendelson; Nathan Keller
Journal:  J Clin Microbiol       Date:  2011-05-11       Impact factor: 5.948

4.  Dissemination of blaKPC-2 by the spread of Klebsiella pneumoniae clonal complex 258 clones (ST258, ST11, ST437) and plasmids (IncFII, IncN, IncL/M) among Enterobacteriaceae species in Brazil.

Authors:  Leonardo Neves Andrade; Tânia Curiao; Joseane Cristina Ferreira; Juliana Mucedola Longo; Eduardo Carneiro Clímaco; Roberto Martinez; Fernando Bellissimo-Rodrigues; Aníbal Basile-Filho; Marco Antônio Evaristo; Pedro F Del Peloso; Vanessa Bley Ribeiro; Afonso Luis Barth; Milena Cristina Paula; Fernando Baquero; Rafael Cantón; Ana Lúcia da Costa Darini; Teresa M Coque
Journal:  Antimicrob Agents Chemother       Date:  2011-05-16       Impact factor: 5.191

5.  Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

Authors:  Laure Diancourt; Virginie Passet; Jan Verhoef; Patrick A D Grimont; Sylvain Brisse
Journal:  J Clin Microbiol       Date:  2005-08       Impact factor: 5.948

6.  Multiplex real-time PCR assay for detection and classification of Klebsiella pneumoniae carbapenemase gene (bla KPC) variants.

Authors:  Liang Chen; José R Mediavilla; Andrea Endimiani; Marnie E Rosenthal; Yanan Zhao; Robert A Bonomo; Barry N Kreiswirth
Journal:  J Clin Microbiol       Date:  2010-12-01       Impact factor: 5.948

7.  Outbreak of colistin-resistant, carbapenem-resistant Klebsiella pneumoniae in metropolitan Detroit, Michigan.

Authors:  Dror Marchaim; Teena Chopra; Jason M Pogue; Federico Perez; Andrea M Hujer; Susan Rudin; Andrea Endimiani; Shiri Navon-Venezia; Jatinder Hothi; Jessica Slim; Christopher Blunden; Maryann Shango; Paul R Lephart; Hossein Salimnia; Deborah Reid; Judy Moshos; Wasif Hafeez; Suchitha Bheemreddy; Ting-Yi Chen; Sorabh Dhar; Robert A Bonomo; Keith S Kaye
Journal:  Antimicrob Agents Chemother       Date:  2010-11-29       Impact factor: 5.191

8.  Rapid detection of blaKPC carbapenemase genes by real-time PCR.

Authors:  Musa Hindiyeh; Gill Smollen; Zehava Grossman; Daniela Ram; Yehudit Davidson; Fernando Mileguir; Marina Vax; Debbie Ben David; Ilana Tal; Galia Rahav; Ari Shamiss; Ella Mendelson; Nathan Keller
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948

9.  Molecular epidemiology of KPC-producing Klebsiella pneumoniae isolates in the United States: clonal expansion of multilocus sequence type 258.

Authors:  Brandon Kitchel; J Kamile Rasheed; Jean B Patel; Arjun Srinivasan; Shiri Navon-Venezia; Yehuda Carmeli; Alma Brolund; Christian G Giske
Journal:  Antimicrob Agents Chemother       Date:  2009-06-08       Impact factor: 5.191

10.  Worldwide diversity of Klebsiella pneumoniae that produce beta-lactamase blaKPC-2 gene.

Authors:  Gaelle Cuzon; Thierry Naas; HaVy Truong; Maria Virginia Villegas; Karin T Wisell; Yehuda Carmeli; Ana C Gales; Shiri Navon Venezia; John P Quinn; Patrice Nordmann
Journal:  Emerg Infect Dis       Date:  2010-09       Impact factor: 6.883
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  31 in total

1.  Complete nucleotide sequence of a blaKPC-harboring IncI2 plasmid and its dissemination in New Jersey and New York hospitals.

Authors:  Liang Chen; Kalyan D Chavda; Nahed Al Laham; Roberto G Melano; Michael R Jacobs; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2013-07-29       Impact factor: 5.191

2.  Doripenem, gentamicin, and colistin, alone and in combinations, against gentamicin-susceptible, KPC-producing Klebsiella pneumoniae strains with various ompK36 genotypes.

Authors:  Cornelius J Clancy; Binghua Hao; Ryan K Shields; Liang Chen; David S Perlin; Barry N Kreiswirth; M Hong Nguyen
Journal:  Antimicrob Agents Chemother       Date:  2014-02-24       Impact factor: 5.191

3.  Association of tellurite resistance with hypervirulent clonal groups of Klebsiella pneumoniae.

Authors:  Virginie Passet; Sylvain Brisse
Journal:  J Clin Microbiol       Date:  2015-01-28       Impact factor: 5.948

4.  Rapid detection and statistical differentiation of KPC gene variants in Gram-negative pathogens by use of high-resolution melting and ScreenClust analyses.

Authors:  Amanda L Roth; Nancy D Hanson
Journal:  J Clin Microbiol       Date:  2012-10-17       Impact factor: 5.948

5.  Antimicrobial treatment and containment measures for an extremely drug-resistant Klebsiella pneumoniae ST101 isolate carrying pKPN101-IT, a novel fully sequenced bla(KPC-2) plasmid.

Authors:  Ilaria Frasson; Enrico Lavezzo; Elisa Franchin; Stefano Toppo; Luisa Barzon; Antonietta Cavallaro; Sara N Richter; Giorgio Palù
Journal:  J Clin Microbiol       Date:  2012-09-12       Impact factor: 5.948

6.  Molecular survey of the dissemination of two blaKPC-harboring IncFIA plasmids in New Jersey and New York hospitals.

Authors:  Liang Chen; Kalyan D Chavda; Roberto G Melano; Tao Hong; Albert D Rojtman; Michael R Jacobs; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2014-02-03       Impact factor: 5.191

7.  Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs.

Authors:  Kalyan D Chavda; Michael J Satlin; Liang Chen; Claudia Manca; Stephen G Jenkins; Thomas J Walsh; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2016-10-21       Impact factor: 5.191

8.  Comparative genomic analysis of KPC-encoding pKpQIL-like plasmids and their distribution in New Jersey and New York Hospitals.

Authors:  Liang Chen; Kalyan D Chavda; Roberto G Melano; Michael R Jacobs; Brian Koll; Tao Hong; Albert D Rojtman; Michael H Levi; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2014-03-10       Impact factor: 5.191

9.  CG258 Klebsiella pneumoniae isolates without β-lactam resistance at the onset of the carbapenem-resistant Enterobacteriaceae epidemic in New York City.

Authors:  Brandon Eilertson; Liang Chen; Audrey Li; Kalyan D Chavda; Bhakti Chavda; Barry N Kreiswirth
Journal:  J Antimicrob Chemother       Date:  2019-01-01       Impact factor: 5.790

Review 10.  Non-phenotypic tests to detect and characterize antibiotic resistance mechanisms in Enterobacteriaceae.

Authors:  Agnese Lupo; Krisztina M Papp-Wallace; Parham Sendi; Robert A Bonomo; Andrea Endimiani
Journal:  Diagn Microbiol Infect Dis       Date:  2013-10-03       Impact factor: 2.803
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