Literature DB >> 27600053

Evaluation of a Multiplex PCR Assay To Rapidly Detect Enterobacteriaceae with a Broad Range of β-Lactamases Directly from Perianal Swabs.

Kalyan D Chavda1, Michael J Satlin2, Liang Chen1, Claudia Manca1, Stephen G Jenkins3, Thomas J Walsh4, Barry N Kreiswirth5.   

Abstract

We developed and evaluated multiplexed molecular beacon probes in a real-time PCR assay to identify prominent extended-spectrum-β-lactamase, plasmid-mediated AmpC β-lactamase (pAmpC) and carbapenemase genes directly from perianal swab specimens within 6 h. We evaluated this assay on 158 perianal swabs collected from hematopoietic stem cell transplant recipients and found that this assay was highly sensitive and specific for detection of CTX-M-, pAmpC-, and KPC-producing Enterobacteriaceae compared to culture on chromogenic agar.
Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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Year:  2016        PMID： 27600053      PMCID： PMC5075117          DOI： 10.1128/AAC.01458-16

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  21 in total

1.  Multiplex PCR for rapid detection of genes encoding acquired metallo-beta-lactamases.

Authors:  Matthew J Ellington; James Kistler; David M Livermore; Neil Woodford
Journal:  J Antimicrob Chemother       Date:  2006-12-21       Impact factor: 5.790

2.  Clinical microbiology costs for methods of active surveillance for Klebsiella pneumoniae carbapenemase-producing Enterobacteriaceae.

Authors:  Amy J Mathers; Melinda Poulter; Dawn Dirks; Joanne Carroll; Costi D Sifri; Kevin C Hazen
Journal:  Infect Control Hosp Epidemiol       Date:  2014-04       Impact factor: 3.254

3.  Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus.

Authors:  Daniel Sinsimer; Surbhi Leekha; Steven Park; Salvatore A E Marras; Larry Koreen; Barbara Willey; Steve Naidich; Kimberlee A Musser; Barry N Kreiswirth
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

4.  Linear-After-The-Exponential (LATE)-PCR: primer design criteria for high yields of specific single-stranded DNA and improved real-time detection.

Authors:  Kenneth E Pierce; J Aquiles Sanchez; John E Rice; Lawrence J Wangh
Journal:  Proc Natl Acad Sci U S A       Date:  2005-06-03       Impact factor: 11.205

5.  Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

Authors:  Liang Chen; Kalyan D Chavda; José R Mediavilla; Yanan Zhao; Henry S Fraimow; Stephen G Jenkins; Michael H Levi; Tao Hong; Albert D Rojtman; Christine C Ginocchio; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2012-03-26       Impact factor: 5.191

6.  Rapid Molecular Diagnostics, Antibiotic Treatment Decisions, and Developing Approaches to Inform Empiric Therapy: PRIMERS I and II.

Authors:  Scott R Evans; Andrea M Hujer; Hongyu Jiang; Kristine M Hujer; Thomas Hall; Christine Marzan; Michael R Jacobs; Rangarajan Sampath; David J Ecker; Claudia Manca; Kalyan Chavda; Pan Zhang; Helen Fernandez; Liang Chen; Jose R Mediavilla; Carol B Hill; Federico Perez; Angela M Caliendo; Vance G Fowler; Henry F Chambers; Barry N Kreiswirth; Robert A Bonomo
Journal:  Clin Infect Dis       Date:  2015-09-25       Impact factor: 9.079

7.  Identification of CTX-M-type extended-spectrum-beta-lactamase genes using real-time PCR and pyrosequencing.

Authors:  Thierry Naas; Cynthia Oxacelay; Patrice Nordmann
Journal:  Antimicrob Agents Chemother       Date:  2006-11-06       Impact factor: 5.191

8.  The global challenge of carbapenem-resistant Enterobacteriaceae in transplant recipients and patients with hematologic malignancies.

Authors:  Michael J Satlin; Stephen G Jenkins; Thomas J Walsh
Journal:  Clin Infect Dis       Date:  2014-01-23       Impact factor: 9.079

9.  A multicentre cohort study on colonization and infection with ESBL-producing Enterobacteriaceae in high-risk patients with haematological malignancies.

Authors:  Maria J G T Vehreschild; Axel Hamprecht; Lisa Peterson; Sören Schubert; Maik Häntschel; Silke Peter; Philippe Schafhausen; Holger Rohde; Marie V Lilienfeld-Toal; Isabelle Bekeredjian-Ding; Johannes Libam; Martin Hellmich; Jörg J Vehreschild; Oliver A Cornely; Harald Seifert
Journal:  J Antimicrob Chemother       Date:  2014-08-06       Impact factor: 5.790

10.  Multiplex real-time PCR for rapid Staphylococcal cassette chromosome mec typing.

Authors:  Liang Chen; José R Mediavilla; Duarte C Oliveira; Barbara M Willey; Herminia de Lencastre; Barry N Kreiswirth
Journal:  J Clin Microbiol       Date:  2009-09-02       Impact factor: 5.948
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  17 in total

1.  Colonization With Levofloxacin-resistant Extended-spectrum β-Lactamase-producing Enterobacteriaceae and Risk of Bacteremia in Hematopoietic Stem Cell Transplant Recipients.

Authors:  Michael J Satlin; Kalyan D Chavda; Thomas M Baker; Liang Chen; Elena Shashkina; Rosemary Soave; Catherine B Small; Samantha E Jacobs; Tsiporah B Shore; Koen van Besien; Lars F Westblade; Audrey N Schuetz; Vance G Fowler; Stephen G Jenkins; Thomas J Walsh; Barry N Kreiswirth
Journal:  Clin Infect Dis       Date:  2018-11-13       Impact factor: 9.079

2.  Biographical Feature: Stephen G. Jenkins, Ph.D., D(ABMM), F(AAM).

Authors:  Alexander J McAdam
Journal:  J Clin Microbiol       Date:  2019-04-26       Impact factor: 5.948

3.  Genomic Characterization of VIM Metallo-β-Lactamase-Producing Alcaligenes faecalis from Gaza, Palestine.

Authors:  Nahed Al Laham; Kalyan D Chavda; Astrid V Cienfuegos-Gallet; Barry N Kreiswirth; Liang Chen
Journal:  Antimicrob Agents Chemother       Date:  2017-10-24       Impact factor: 5.191

4.  Multicenter Clinical and Molecular Epidemiological Analysis of Bacteremia Due to Carbapenem-Resistant Enterobacteriaceae (CRE) in the CRE Epicenter of the United States.

Authors:  Michael J Satlin; Liang Chen; Gopi Patel; Angela Gomez-Simmonds; Gregory Weston; Angela C Kim; Susan K Seo; Marnie E Rosenthal; Steven J Sperber; Stephen G Jenkins; Camille L Hamula; Anne-Catrin Uhlemann; Michael H Levi; Bettina C Fries; Yi-Wei Tang; Stefan Juretschko; Albert D Rojtman; Tao Hong; Barun Mathema; Michael R Jacobs; Thomas J Walsh; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2017-03-24       Impact factor: 5.191

5.  Genomic Characterization of Two KPC-Producing Klebsiella Isolates Collected in 1997 in New York City.

Authors:  Brandon Eilertson; Liang Chen; Kalyan D Chavda; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2017-03-24       Impact factor: 5.191

Review 6.  Prophylactic Measures During Induction for Acute Myeloid Leukemia.

Authors:  Matthew W McCarthy; Thomas J Walsh
Journal:  Curr Oncol Rep       Date:  2017-03       Impact factor: 5.075

7.  Genomic Investigation of a Putative Endoscope-Associated Carbapenem-Resistant Enterobacter cloacae Outbreak Reveals a Wide Diversity of Circulating Strains and Resistance Mutations.

Authors:  Shawn E Hawken; Laraine L Washer; Christopher L Williams; Duane W Newton; Evan S Snitkin
Journal:  Clin Infect Dis       Date:  2018-01-18       Impact factor: 9.079

8.  CG258 Klebsiella pneumoniae isolates without β-lactam resistance at the onset of the carbapenem-resistant Enterobacteriaceae epidemic in New York City.

Authors:  Brandon Eilertson; Liang Chen; Audrey Li; Kalyan D Chavda; Bhakti Chavda; Barry N Kreiswirth
Journal:  J Antimicrob Chemother       Date:  2019-01-01       Impact factor: 5.790

9.  Molecular Epidemiology, Natural History, and Long-Term Outcomes of Multidrug-Resistant Enterobacterales Colonization and Infections Among Solid Organ Transplant Recipients.

Authors:  M Hong Nguyen; Ryan K Shields; Liang Chen; A William Pasculle; Binghua Hao; Shaoji Cheng; Jonathan Sun; Ellen G Kline; Barry N Kreiswirth; Cornelius J Clancy
Journal:  Clin Infect Dis       Date:  2022-02-11       Impact factor: 9.079

10.  Colonization With Fluoroquinolone-Resistant Enterobacterales Decreases the Effectiveness of Fluoroquinolone Prophylaxis in Hematopoietic Cell Transplant Recipients.

Authors:  Michael J Satlin; Liang Chen; Claire Douglass; Michael Hovan; Emily Davidson; Rosemary Soave; Marisa La Spina; Alexandra Gomez-Arteaga; Koen van Besien; Sebastian Mayer; Adrienne Phillips; Jing-Mei Hsu; Rianna Malherbe; Catherine B Small; Stephen G Jenkins; Lars F Westblade; Barry N Kreiswirth; Thomas J Walsh
Journal:  Clin Infect Dis       Date:  2021-10-05       Impact factor: 20.999
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