Literature DB >> 2243781

The enhancers and promoters of the Xenopus laevis ribosomal spacer are associated with histones upon active transcription of the ribosomal genes.

S I Dimitrov1, L Karagyozov, D Angelov, I G Pashev.   

Abstract

The presence of histones on the enhancer-promoter region of the X.laevis ribosomal spacer has been studied in embryos at stage 40, where the ribosomal genes are actively transcribed. Isolated tadpole nuclei were either fixed with formaldehyde or irradiated with UV laser to crosslink histones to DNA. The purified protein-DNA complexes were immunoprecipitated with antibodies to the histones H1, H2A and H4 and the DNA fragments carrying the respective histones were analyzed for the presence of spacer enhancer-promoter sequences by hybridization to specific DNA probe. The two independent crosslinking procedures revealed the presence of these DNA sequences in the precipitated DNA. The quantitative analysis of the UV laser-crosslinked complexes showed that histones H2A and H4 were associated with enhancer-promoter DNA in amounts similar to those found for bulk DNA, whilst the content of H1 was reduced.

Entities:  

Mesh：

Substances：

Year:  1990        PMID： 2243781      PMCID： PMC332518          DOI： 10.1093/nar/18.21.6393

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  33 in total

1.  Laser-induced crosslinking of histones to DNA in chromatin and core particles: implications in studying histone-DNA interactions.

Authors:  S I Dimitrov; V R Russanova; D Angelov; I G Pashev
Journal:  Nucleic Acids Res       Date:  1989-12-11       Impact factor: 16.971

2.  Reversible changes in nucleosome structure and histone H3 accessibility in transcriptionally active and inactive states of rDNA chromatin.

Authors:  C P Prior; C R Cantor; E M Johnson; V C Littau; V G Allfrey
Journal:  Cell       Date:  1983-10       Impact factor: 41.582

3.  Coupled demethylation of sites in a conserved sequence of Xenopus ribosomal DNA.

Authors:  A La Volpe; M Taggart; D Macleod; A Bird
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1983

4.  Structure of the active nucleolar chromatin of Xenopus laevis Oocytes.

Authors:  P Labhart; T Koller
Journal:  Cell       Date:  1982-02       Impact factor: 41.582

5.  Chromatin structure of hsp 70 genes, activated by heat shock: selective removal of histones from the coding region and their absence from the 5' region.

Authors:  V L Karpov; O V Preobrazhenskaya; A D Mirzabekov
Journal:  Cell       Date:  1984-02       Impact factor: 41.582

6.  Compact structure of ribosomal chromatin in Xenopus laevis.

Authors:  C Spadafora; M Crippa
Journal:  Nucleic Acids Res       Date:  1984-03-26       Impact factor: 16.971

7.  A transcriptional function for the repetitive ribosomal spacer in Xenopus laevis.

Authors:  T Moss
Journal:  Nature       Date:  1983 Mar 17-23       Impact factor: 49.962

8.  Nucleosome structure of Xenopus oocyte amplified ribosomal genes.

Authors:  R Reeves
Journal:  Biochemistry       Date:  1978-11-14       Impact factor: 3.162

9.  DNaseI-hypersensitive sites at promoter-like sequences in the spacer of Xenopus laevis and Xenopus borealis ribosomal DNA.

Authors:  A La Volpe; M Taggart; B McStay; A Bird
Journal:  Nucleic Acids Res       Date:  1983-08-25       Impact factor: 16.971

10.  Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.

Authors:  T Moss
Journal:  Cell       Date:  1982-10       Impact factor: 41.582
View more
  10 in total

1.  The transcriptionally-active MMTV promoter is depleted of histone H1.

Authors:  E H Bresnick; M Bustin; V Marsaud; H Richard-Foy; G L Hager
Journal:  Nucleic Acids Res       Date:  1992-01-25       Impact factor: 16.971

2.  Is higher-order structure conserved in eukaryotic ribosomal DNA intergenic spacers?

Authors:  G D Baldridge; M W Dalton; A M Fallon
Journal:  J Mol Evol       Date:  1992-12       Impact factor: 2.395

3.  Persistent interactions of core histone tails with nucleosomal DNA following acetylation and transcription factor binding.

Authors:  V Mutskov; D Gerber; D Angelov; J Ausio; J Workman; S Dimitrov
Journal:  Mol Cell Biol       Date:  1998-11       Impact factor: 4.272

4.  Prolonged glucocorticoid exposure dephosphorylates histone H1 and inactivates the MMTV promoter.

Authors:  H L Lee; T K Archer
Journal:  EMBO J       Date:  1998-03-02       Impact factor: 11.598

5.  The DNA supercoiling architecture induced by the transcription factor xUBF requires three of its five HMG-boxes.

Authors:  V Y Stefanovsky; D P Bazett-Jones; G Pelletier; T Moss
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

6.  Putative tfIIs gene of Sulfolobus acidocaldarius encoding an archaeal transcription elongation factor is situated directly downstream of the gene for a small subunit of DNA-dependent RNA polymerase.

Authors:  D Langer; W Zillig
Journal:  Nucleic Acids Res       Date:  1993-05-11       Impact factor: 16.971

7.  Mapping and positioning DNA-binding proteins along genomic DNA. Structure of D. melanogaster ribosomal 'Alu-repeats' and 1.688 satellite chromatin.

Authors:  S V Belikov; A I Belgovsky; M P Partolina; V L Karpov; A D Mirzabekov
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971

8.  Modeling of DNA local parameters predicts encrypted architectural motifs in Xenopus laevis ribosomal gene promoter.

Authors:  M Roux-Rouquie; M Marilley
Journal:  Nucleic Acids Res       Date:  2000-09-15       Impact factor: 16.971

9.  Different chromatin structures along the spacers flanking active and inactive Xenopus rRNA genes.

Authors:  R Lucchini; J M Sogo
Journal:  Mol Cell Biol       Date:  1992-10       Impact factor: 4.272

10.  Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini.

Authors:  L J Juan; R T Utley; C C Adams; M Vettese-Dadey; J L Workman
Journal:  EMBO J       Date:  1994-12-15       Impact factor: 11.598
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.