Literature DB >> 22378915

Ultrarapid detection of blaKPC₁/₂-₁₂ from perirectal and nasal swabs by use of real-time PCR.

Sara N Richter1, Ilaria Frasson, Maria Angela Biasolo, Andrea Bartolini, Antonietta Cavallaro, Giorgio Palù.   

Abstract

The novel real-time PCR assay developed as described here was able to detect bla(KPC1/2-12) (bla(KPC-1/2) to bla(KPC-12)) from easily available clinical specimens in less than 2 h. The genotypic assay was highly sensitive (100%) and specific (98%). In some cases, it was able to detect bla(KPC) 48 h before positive detection by standard phenotypic assay on patients who were monitored daily. The high sensitivity and rapidity of the molecular method make it the method of choice for KPC surveillance and, thus, containment purposes.

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Year:  2012        PMID： 22378915      PMCID： PMC3347151          DOI： 10.1128/JCM.00195-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Real-time detection of blaKPC in clinical samples and surveillance specimens.

Authors:  Kathy A Mangold; Kristine Santiano; Ronit Broekman; Catherine A Krafft; Barbara Voss; Vivien Wang; Donna M Hacek; Elena A Usacheva; Richard B Thomson; Karen L Kaul; Lance R Peterson
Journal:  J Clin Microbiol       Date:  2011-07-06       Impact factor: 5.948

2.  Rapid increase of carbapenemase-producing Klebsiella pneumoniae strains in a large Italian hospital: surveillance period 1 March - 30 September 2010.

Authors:  P Gaibani; S Ambretti; A Berlingeri; F Gelsomino; A Bielli; M P Landini; V Sambri
Journal:  Euro Surveill       Date:  2011-02-24

3.  Rapid detection of blaKPC carbapenemase genes by internally controlled real-time PCR assay using bactec blood culture bottles.

Authors:  Musa Hindiyeh; Gill Smollan; Zehava Grossman; Daniela Ram; Jana Robinov; Natasha Belausov; Debbie Ben-David; Ilana Tal; Yehudit Davidson; Ari Shamiss; Ella Mendelson; Nathan Keller
Journal:  J Clin Microbiol       Date:  2011-05-11       Impact factor: 5.948

Review 4.  Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging cause of multidrug-resistant infection.

Authors:  Elizabeth B Hirsch; Vincent H Tam
Journal:  J Antimicrob Chemother       Date:  2010-04-08       Impact factor: 5.790

5.  High-level carbapenem resistance in a Klebsiella pneumoniae clinical isolate is due to the combination of bla(ACT-1) beta-lactamase production, porin OmpK35/36 insertional inactivation, and down-regulation of the phosphate transport porin phoe.

Authors:  Frank M Kaczmarek; Fadia Dib-Hajj; Wenchi Shang; Thomas D Gootz
Journal:  Antimicrob Agents Chemother       Date:  2006-10       Impact factor: 5.191

6.  Assessment of CMV load in solid organ transplant recipients by pp65 antigenemia and real-time quantitative DNA PCR assay: correlation with pp67 RNA detection.

Authors:  Carlo Mengoli; Riccardo Cusinato; Maria A Biasolo; Simone Cesaro; Cristina Parolin; Giorgio Palù
Journal:  J Med Virol       Date:  2004-09       Impact factor: 2.327

7.  Rapid detection of blaKPC carbapenemase genes by real-time PCR.

Authors:  Musa Hindiyeh; Gill Smollen; Zehava Grossman; Daniela Ram; Yehudit Davidson; Fernando Mileguir; Marina Vax; Debbie Ben David; Ilana Tal; Galia Rahav; Ari Shamiss; Ella Mendelson; Nathan Keller
Journal:  J Clin Microbiol       Date:  2008-07-09       Impact factor: 5.948
  7 in total
  11 in total

1.  Clinical Performance of Check-Direct CPE, a Multiplex PCR for Direct Detection of bla(KPC), bla(NDM) and/or bla(VIM), and bla(OXA)-48 from Perirectal Swabs.

Authors:  Anna F Lau; Gary A Fahle; Margaret A Kemp; Agatha N Jassem; John P Dekker; Karen M Frank
Journal:  J Clin Microbiol       Date:  2015-09-02       Impact factor: 5.948

Review 2.  Intestinal Carriage of Carbapenemase-Producing Organisms: Current Status of Surveillance Methods.

Authors:  Roberto Viau; Karen M Frank; Michael R Jacobs; Brigid Wilson; Keith Kaye; Curtis J Donskey; Federico Perez; Andrea Endimiani; Robert A Bonomo
Journal:  Clin Microbiol Rev       Date:  2016-01       Impact factor: 26.132

3.  Rapid and direct real-time detection of blaKPC and blaNDM from surveillance samples.

Authors:  Shawn Vasoo; Scott A Cunningham; Peggy C Kohner; Jayawant N Mandrekar; Karen Lolans; Mary K Hayden; Robin Patel
Journal:  J Clin Microbiol       Date:  2013-08-21       Impact factor: 5.948

4.  Detection of colonization by carbapenemase-producing Gram-negative Bacilli in patients by use of the Xpert MDRO assay.

Authors:  Fred C Tenover; Rafael Canton; JoAnn Kop; Ryan Chan; Jamie Ryan; Fred Weir; Patricia Ruiz-Garbajosa; Vincent LaBombardi; David H Persing
Journal:  J Clin Microbiol       Date:  2013-09-04       Impact factor: 5.948

5.  Improved blood culture workflow for faster identification of KPC-producing Enterobacterales.

Authors:  Bruna Mara Silva Seco; Juliana Coutinho Campos; Darlan Augusto da Costa Rocha; Aline Valerio de Lima; Fernanda Filomena de Oliveira; Mara Elisa Borsato Lemo; Suely Carlos Ferreira Sampaio; Jorge Luiz Mello Sampaio
Journal:  Braz J Microbiol       Date:  2018-12-06       Impact factor: 2.476

6.  Antimicrobial treatment and containment measures for an extremely drug-resistant Klebsiella pneumoniae ST101 isolate carrying pKPN101-IT, a novel fully sequenced bla(KPC-2) plasmid.

Authors:  Ilaria Frasson; Enrico Lavezzo; Elisa Franchin; Stefano Toppo; Luisa Barzon; Antonietta Cavallaro; Sara N Richter; Giorgio Palù
Journal:  J Clin Microbiol       Date:  2012-09-12       Impact factor: 5.948

7.  Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.

Authors:  Scott A Cunningham; Tabassum Noorie; Daniele Meunier; Neil Woodford; Robin Patel
Journal:  J Clin Microbiol       Date:  2013-01-23       Impact factor: 5.948

8.  Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

Authors:  Liang Chen; Kalyan D Chavda; José R Mediavilla; Yanan Zhao; Henry S Fraimow; Stephen G Jenkins; Michael H Levi; Tao Hong; Albert D Rojtman; Christine C Ginocchio; Robert A Bonomo; Barry N Kreiswirth
Journal:  Antimicrob Agents Chemother       Date:  2012-03-26       Impact factor: 5.191

9.  A combined disk test for direct differentiation of carbapenemase-producing enterobacteriaceae in surveillance rectal swabs.

Authors:  Spyros Pournaras; Olympia Zarkotou; Aggeliki Poulou; Ioulia Kristo; Georgia Vrioni; Katerina Themeli-Digalaki; Athanassios Tsakris
Journal:  J Clin Microbiol       Date:  2013-07-10       Impact factor: 5.948

10.  Comparison of phenotypic methods for the detection of carbapenem non-susceptible Enterobacteriaceae.

Authors:  Andrea Bartolini; Ilaria Frasson; Antonietta Cavallaro; Sara N Richter; Giorgio Palù
Journal:  Gut Pathog       Date:  2014-05-19       Impact factor: 4.181
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