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Literature DB >> 23345290

Rapid and simultaneous detection of genes encoding Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) in Gram-negative bacilli.

Scott A Cunningham¹, Tabassum Noorie, Daniele Meunier, Neil Woodford, Robin Patel.

Abstract

We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.

Entities: Disease

Mesh：

Substances：

Year: 2013 PMID： 23345290 PMCID： PMC3666784 DOI： 10.1128/JCM.03062-12

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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