Literature DB >> 22298299

In-cell solid-state NMR as a tool to study proteins in large complexes.

Sina Reckel1, Jakob J Lopez, Frank Löhr, Clemens Glaubitz, Volker Dötsch.   

Abstract

A major limitation of solution NMR is molecular tumbling, which is often too slow for detection. Here we demonstrate that solid-state NMR spectroscopy in combination with flash freezing of cells can be used to detect proteins in the cellular environment and provides information on backbone chemical shifts.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Mesh:

Substances:

Year:  2012        PMID: 22298299     DOI: 10.1002/cbic.201100721

Source DB:  PubMed          Journal:  Chembiochem        ISSN: 1439-4227            Impact factor:   3.164


  21 in total

Review 1.  In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.

Authors:  Xavier L Warnet; Alexandre A Arnold; Isabelle Marcotte; Dror E Warschawski
Journal:  Biophys J       Date:  2015-12-15       Impact factor: 4.033

Review 2.  A Unique Tool for Cellular Structural Biology: In-cell NMR.

Authors:  Enrico Luchinat; Lucia Banci
Journal:  J Biol Chem       Date:  2015-12-16       Impact factor: 5.157

3.  Secondary structural analysis of proteins based on (13)C chemical shift assignments in unresolved solid-state NMR spectra enhanced by fragmented structure database.

Authors:  Keisuke Ikeda; Ayako Egawa; Toshimichi Fujiwara
Journal:  J Biomol NMR       Date:  2012-12-29       Impact factor: 2.835

Review 4.  New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR.

Authors:  Rivkah Rogawski; Ann E McDermott
Journal:  Arch Biochem Biophys       Date:  2017-06-13       Impact factor: 4.013

5.  Probing protein quinary interactions by in-cell nuclear magnetic resonance spectroscopy.

Authors:  Subhabrata Majumder; Jing Xue; Christopher M DeMott; Sergey Reverdatto; David S Burz; Alexander Shekhtman
Journal:  Biochemistry       Date:  2015-04-27       Impact factor: 3.162

6.  Characterizing proteins in a native bacterial environment using solid-state NMR spectroscopy.

Authors:  Siddarth Narasimhan; Cecilia Pinto; Alessandra Lucini Paioni; Johan van der Zwan; Gert E Folkers; Marc Baldus
Journal:  Nat Protoc       Date:  2021-01-13       Impact factor: 13.491

7.  Quantitation of recombinant protein in whole cells and cell extracts via solid-state NMR spectroscopy.

Authors:  Erica P Vogel; David P Weliky
Journal:  Biochemistry       Date:  2013-06-17       Impact factor: 3.162

Review 8.  Hidden motions and motion-induced invisibility: Dynamics-based spectral editing in solid-state NMR.

Authors:  Irina Matlahov; Patrick C A van der Wel
Journal:  Methods       Date:  2018-04-24       Impact factor: 3.608

9.  Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization.

Authors:  Kazutoshi Yamamoto; Marc A Caporini; Sang-Choul Im; Lucy Waskell; Ayyalusamy Ramamoorthy
Journal:  Biochim Biophys Acta       Date:  2014-07-11

10.  Sensitivity-enhanced NMR reveals alterations in protein structure by cellular milieus.

Authors:  Kendra K Frederick; Vladimir K Michaelis; Björn Corzilius; Ta-Chung Ong; Angela C Jacavone; Robert G Griffin; Susan Lindquist
Journal:  Cell       Date:  2015-10-08       Impact factor: 41.582

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.