Literature DB >> 22281741

Global radiation damage at 300 and 260 K with dose rates approaching 1 MGy s⁻¹.

Matthew Warkentin1, Ryan Badeau, Jesse B Hopkins, Anne M Mulichak, Lisa J Keefe, Robert E Thorne.   

Abstract

Global radiation damage to 19 thaumatin crystals has been measured using dose rates from 3 to 680 kGy s⁻¹. At room temperature damage per unit dose appears to be roughly independent of dose rate, suggesting that the timescales for important damage processes are less than ∼1 s. However, at T = 260 K approximately half of the global damage manifested at dose rates of ∼10 kGy s⁻¹ can be outrun by collecting data at 680 kGy s⁻¹. Appreciable sample-to-sample variability in global radiation sensitivity at fixed dose rate is observed. This variability cannot be accounted for by errors in dose calculation, crystal slippage or the size of the data sets in the assay.

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Year:  2012        PMID: 22281741      PMCID: PMC3266852          DOI: 10.1107/S0907444911052085

Source DB:  PubMed          Journal:  Acta Crystallogr D Biol Crystallogr        ISSN: 0907-4449


  40 in total

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3.  Non-invasive measurement of X-ray beam heating on a surrogate crystal sample.

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4.  Reduction of X-ray-induced radiation damage of macromolecular crystals by data collection at 15 K: a systematic study.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2007-02-21

5.  Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography.

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6.  Slow cooling of protein crystals.

Authors:  Matthew Warkentin; Robert E Thorne
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8.  Radiation damage in protein crystals is reduced with a micron-sized X-ray beam.

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Journal:  Proc Natl Acad Sci U S A       Date:  2011-03-28       Impact factor: 11.205

9.  Primary radiation damage of protein crystals by an intense synchrotron X-ray beam.

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Journal:  J Synchrotron Radiat       Date:  2000-09-01       Impact factor: 2.616

10.  Room-temperature scavengers for macromolecular crystallography: increased lifetimes and modified dose dependence of the intensity decay.

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  15 in total

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2.  Spatial distribution of radiation damage to crystalline proteins at 25-300 K.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2012-08-18

3.  Global radiation damage: temperature dependence, time dependence and how to outrun it.

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Journal:  J Synchrotron Radiat       Date:  2012-11-29       Impact factor: 2.616

4.  Identification of patterns in diffraction intensities affected by radiation exposure.

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5.  Quantifying radiation damage in biomolecular small-angle X-ray scattering.

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Journal:  J Appl Crystallogr       Date:  2016-05-04       Impact factor: 3.304

6.  Instrumentation and experimental procedures for robust collection of X-ray diffraction data from protein crystals across physiological temperatures.

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Review 7.  Practical macromolecular cryocrystallography.

Authors:  J W Pflugrath
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8.  Matching X-ray beam and detector properties to protein crystals of different perfection.

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Journal:  J Synchrotron Radiat       Date:  2014-03-18       Impact factor: 2.616

9.  A survey of global radiation damage to 15 different protein crystal types at room temperature: a new decay model.

Authors:  Ricardo Miguel Ferraz Leal; Gleb Bourenkov; Silvia Russi; Alexander N Popov
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10.  Exploiting fast detectors to enter a new dimension in room-temperature crystallography.

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