BACKGROUND: To support pediatric study, a method to determine lumefantrine (LF) with small sample volume is needed. Matrix effect (ME) is a daunting issue in LF quantification in human plasma with LC-MS/MS. RESULTS: Here we report an LC-MS/MS method with a deuterated LF as the internal standard (IS). Plasma sample (25 µl) was acidified with 5% formic acid prior to extraction with ethyl acetate. The recovery was over 80%. The absolute ME was within the range of 100 ± 8% for both LF and the IS, but cumulative ME was observed via large variation of IS signal. The cumulative ME and ionization saturation were overcome with the co-eluting LF-D(9) as the IS. The linear range of calibration curve was 50-20,000 ng/ml. CONCLUSION: ME and ionization saturation was overcome with a deuterated IS. The method utilized a small sample volume, suitable for pediatric study with capillary tube blood collection method.
BACKGROUND: To support pediatric study, a method to determine lumefantrine (LF) with small sample volumeis needed. Matrix effect (ME) is a daunting issue in LF quantification in human plasma with LC-MS/MS. RESULTS: Here we report an LC-MS/MS method with a deuterated LF as the internal standard (IS). Plasma sample (25 µl) was acidified with 5% formic acid prior to extraction with ethyl acetate. The recovery was over 80%. The absolute ME was within the range of 100 ± 8% for both LF and the IS, but cumulative ME was observed via large variation of IS signal. The cumulative ME and ionization saturation were overcome with the co-eluting LF-D(9) as the IS. The linear range of calibration curve was 50-20,000 ng/ml. CONCLUSION:ME and ionization saturation was overcome with a deuterated IS. The method utilized a small sample volume, suitable for pediatric study with capillary tube blood collection method.
Authors: A Annerberg; T Singtoroj; P Tipmanee; N J White; N P J Day; N Lindegårdh Journal: J Chromatogr B Analyt Technol Biomed Life Sci Date: 2005-08-05 Impact factor: 3.205
Authors: Insaf F Khalil; Ulla Abildrup; Lene H Alifrangis; Deogratius Maiga; Michael Alifrangis; Lotte Hoegberg; Lasse S Vestergaard; Ola Per-Eric Persson; Nyagonde Nyagonde; Martha M Lemnge; Thor G Theander; Ib C Bygbjerg Journal: J Pharm Biomed Anal Date: 2010-08-17 Impact factor: 3.935
Authors: Elizabeth A Ashley; Kasia Stepniewska; Niklas Lindegårdh; Rose McGready; Anna Annerberg; Robert Hutagalung; Thida Singtoroj; Gilvary Hla; Al Brockman; Stephane Proux; Jahser Wilahphaingern; Pratap Singhasivanon; Nicholas J White; François Nosten Journal: Trop Med Int Health Date: 2007-02 Impact factor: 2.622
Authors: D Blessborn; S Römsing; A Annerberg; D Sundquist; A Björkman; N Lindegardh; Y Bergqvist Journal: J Pharm Biomed Anal Date: 2007-07-20 Impact factor: 3.935
Authors: Jane Achan; Abel Kakuru; Gloria Ikilezi; Theodore Ruel; Tamara D Clark; Christian Nsanzabana; Edwin Charlebois; Francesca Aweeka; Grant Dorsey; Philip J Rosenthal; Diane Havlir; Moses R Kamya Journal: N Engl J Med Date: 2012-11-29 Impact factor: 91.245
Authors: Matthew M Ippolito; Julia C Pringle; Mwiche Siame; Ben Katowa; Ozkan Aydemir; Peter O Oluoch; Liusheng Huang; Francesca T Aweeka; Jeffrey A Bailey; Jonathan J Juliano; Steven R Meshnick; Theresa A Shapiro; William J Moss; Philip E Thuma Journal: Am J Trop Med Hyg Date: 2020-10-15 Impact factor: 3.707
Authors: Liusheng Huang; Vincent Carey; Jane C Lindsey; Florence Marzan; David Gingrich; Bobbie Graham; Linda Barlow-Mosha; Phionah K Ssemambo; Portia Kamthunzi; Sharon Nachman; Sunil Parikh; Francesca T Aweeka Journal: PLoS One Date: 2017-10-24 Impact factor: 3.240