AIM: Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume. RESULTS: We report a sensitive LC-MS/MS method for quantitation of PQ with only 25 µl human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI(+) as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5-250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children. CONCLUSION: The developed LC-MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.
AIM: Determination of piperaquine (PQ) in pediatric plasma requires a method with a small sample volume. RESULTS: We report a sensitive LC-MS/MS method for quantitation of PQ with only 25 µl human plasma. Using a deuterated internal standard (PQ-d6), an analytical PFP column, APCI(+) as the ion source and MRM (535/288 for PQ and 541/294 for the IS) for detection, the method has a linear calibration range of 1.5-250 ng/ml with a runtime of 3.0 min per sample. The method was applied to plasma samples from children. CONCLUSION: The developed LC-MS/MS method is suitable for pediatric studies with small volume plasma samples collected via capillary tubes. One limitation was the performance of PFP columns varied among different brands.
Authors: Darren Creek; Victor Bigira; Emmanuel Arinaitwe; Humphrey Wanzira; Abel Kakuru; Jordan Tappero; Moses R Kamya; Grant Dorsey; Taylor G Sandison Journal: Am J Trop Med Hyg Date: 2010-10 Impact factor: 2.345
Authors: Elizabeth A Ashley; Kasia Stepniewska; Niklas Lindegardh; Anna Annerberg; Joel Tarning; Rose McGready; Lucy Phaiphun; Pratap Singhasivanon; Nicholas J White; François Nosten Journal: Eur J Clin Pharmacol Date: 2010-03-19 Impact factor: 2.953
Authors: Joaniter Nankabirwa; Bonnie Cundill; Sian Clarke; Narcis Kabatereine; Philip J Rosenthal; Grant Dorsey; Simon Brooker; Sarah G Staedke Journal: PLoS One Date: 2010-10-19 Impact factor: 3.240
Authors: Darren J Creek; Victor Bigira; Shelley McCormack; Emmanuel Arinaitwe; Humphrey Wanzira; Abel Kakuru; Jordan W Tappero; Taylor G Sandison; Niklas Lindegardh; Francois Nosten; Francesca T Aweeka; Sunil Parikh Journal: J Infect Dis Date: 2013-02-27 Impact factor: 5.226
Authors: Jane Achan; Abel Kakuru; Gloria Ikilezi; Theodore Ruel; Tamara D Clark; Christian Nsanzabana; Edwin Charlebois; Francesca Aweeka; Grant Dorsey; Philip J Rosenthal; Diane Havlir; Moses R Kamya Journal: N Engl J Med Date: 2012-11-29 Impact factor: 91.245
Authors: Erika Wallender; Nan Zhang; Melissa Conrad; Abel Kakuru; Mary Muhindo; Patrick Tumwebaze; Richard Kajubi; Daniel Mota; Jennifer Legac; Prasanna Jagannathan; Diane Havlir; Moses Kamya; Grant Dorsey; Francesca Aweeka; Philip J Rosenthal; Rada M Savic Journal: Antimicrob Agents Chemother Date: 2019-01-29 Impact factor: 5.191
Authors: R Kajubi; L Huang; P Jagannathan; N Chamankhah; M Were; T Ruel; C A Koss; A Kakuru; N Mwebaza; M Kamya; D Havlir; G Dorsey; P J Rosenthal; F T Aweeka Journal: Clin Pharmacol Ther Date: 2017-05-30 Impact factor: 6.875
Authors: Rada M Savic; Prasanna Jagannathan; Richard Kajubi; Liusheng Huang; Nan Zhang; Moses Were; Abel Kakuru; Mary K Muhindo; Norah Mwebaza; Erika Wallender; Tamara D Clark; Bishop Opira; Moses Kamya; Diane V Havlir; Philip J Rosenthal; Grant Dorsey; Francesca T Aweeka Journal: Clin Infect Dis Date: 2018-09-14 Impact factor: 9.079
Authors: Prasanna Jagannathan; Katherine Bowen; Felistas Nankya; Tara I McIntyre; Ann Auma; Samuel Wamala; Esther Sikyomu; Kate Naluwu; Mayimuna Nalubega; Michelle J Boyle; Lila A Farrington; Victor Bigira; James Kapisi; Fran Aweeka; Bryan Greenhouse; Moses Kamya; Grant Dorsey; Margaret E Feeney Journal: J Infect Dis Date: 2016-04-10 Impact factor: 5.226
Authors: Lila A Farrington; Prasanna Jagannathan; Tara I McIntyre; Hilary M Vance; Katherine Bowen; Michelle J Boyle; Felistas Nankya; Samuel Wamala; Ann Auma; Mayimuna Nalubega; Esther Sikyomu; Kate Naluwu; Victor Bigira; James Kapisi; Grant Dorsey; Moses R Kamya; Margaret E Feeney Journal: J Infect Dis Date: 2015-12-13 Impact factor: 5.226
Authors: Melissa D Conrad; Daniel Mota; Marissa Foster; Stephen Tukwasibwe; Jennifer Legac; Patrick Tumwebaze; Meghan Whalen; Abel Kakuru; Patience Nayebare; Erika Wallender; Diane V Havlir; Prasanna Jagannathan; Liusheng Huang; Francesca Aweeka; Moses R Kamya; Grant Dorsey; Philip J Rosenthal Journal: J Infect Dis Date: 2017-11-15 Impact factor: 5.226