OBJECTIVE: To assess the developmental kinetics of human embryos and their ability to develop to morphologically normal blastocysts. DESIGN: Experimental study on human embryos donated for research using a time-lapse imaging system based on individual embryo culture in poly(dimethylsiloxane) microwells and monitored using a microscope inside the incubator. SETTING: Private fertility clinic. PATIENT(S): Surplus embryos donated by couples after undergoing fertility treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Blastocyst score and times required from beginning to completion of the second and third mitotic divisions. RESULT(S): The time required for completion of the second division (the three- to four-cell stage) was shorter in embryos that developed to high-scoring blastocysts (0.7 hours, n = 17) than in those forming low-scoring blastocysts (3.7 hours, n = 24). Similarly, the mean time required to completion of the third division (five- to eight-cell stage) was also significantly shorter in embryos forming high-scoring blastocysts (5.7 hours) than among those forming low-scoring blastocysts (16.9 hours). CONCLUSION(S): Individual embryos with the potential to develop to high-scoring blastocysts could be selected at 2-3 days of culture using this system by examining the times required to complete the second and third mitotic divisions.
OBJECTIVE: To assess the developmental kinetics of human embryos and their ability to develop to morphologically normal blastocysts. DESIGN: Experimental study on human embryos donated for research using a time-lapse imaging system based on individual embryo culture in poly(dimethylsiloxane) microwells and monitored using a microscope inside the incubator. SETTING: Private fertility clinic. PATIENT(S): Surplus embryos donated by couples after undergoing fertility treatment. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Blastocyst score and times required from beginning to completion of the second and third mitotic divisions. RESULT(S): The time required for completion of the second division (the three- to four-cell stage) was shorter in embryos that developed to high-scoring blastocysts (0.7 hours, n = 17) than in those forming low-scoring blastocysts (3.7 hours, n = 24). Similarly, the mean time required to completion of the third division (five- to eight-cell stage) was also significantly shorter in embryos forming high-scoring blastocysts (5.7 hours) than among those forming low-scoring blastocysts (16.9 hours). CONCLUSION(S): Individual embryos with the potential to develop to high-scoring blastocysts could be selected at 2-3 days of culture using this system by examining the times required to complete the second and third mitotic divisions.
Authors: Anton Neyer; Martin Zintz; Astrid Stecher; Magnus Bach; Barbara Wirleitner; Nicolas H Zech; Pierre Vanderzwalmen Journal: J Assist Reprod Genet Date: 2015-08-19 Impact factor: 3.412
Authors: T Maezawa; M Yamanaka; S Hashimoto; A Amo; A Ohgaki; Y Nakaoka; A Fukuda; T Ikeda; M Inoue; Y Morimoto Journal: J Assist Reprod Genet Date: 2014-05-25 Impact factor: 3.412