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Literature DB >> 22209075

Assembly of Drosophila centromeric nucleosomes requires CID dimerization.

Weiguo Zhang¹, Serafin U Colmenares, Gary H Karpen.

Abstract

Centromeres are essential chromosomal regions required for kinetochore assembly and chromosome segregation. The composition and organization of centromeric nucleosomes containing the essential histone H3 variant CENP-A (CID in Drosophila) is a fundamental, unresolved issue. Using immunoprecipitation of CID mononucleosomes and cysteine crosslinking, we demonstrate that centromeric nucleosomes contain CID dimers in vivo. Furthermore, CID dimerization and centromeric targeting require a residue implicated in formation of the four-helix bundle, which mediates intranucleosomal H3 dimerization and nucleosome integrity. Taken together, our findings suggest that CID nucleosomes are octameric in vivo and that CID dimerization is essential for correct centromere assembly.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2011 PMID： 22209075 PMCID： PMC3299411 DOI： 10.1016/j.molcel.2011.12.010

Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970

32 in total

1. Crystal structure of the human centromeric nucleosome containing CENP-A.

Authors: Hiroaki Tachiwana; Wataru Kagawa; Tatsuya Shiga; Akihisa Osakabe; Yuta Miya; Kengo Saito; Yoko Hayashi-Takanaka; Takashi Oda; Mamoru Sato; Sam-Yong Park; Hiroshi Kimura; Hitoshi Kurumizaka
Journal: Nature Date: 2011-07-10 Impact factor: 49.962

2. H3.3 is deposited at centromeres in S phase as a placeholder for newly assembled CENP-A in G₁ phase.

Authors: Elaine M Dunleavy; Geneviève Almouzni; Gary H Karpen
Journal: Nucleus Date: 2011 Mar-Apr Impact factor: 4.197

3. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells.

Authors: Satoshi Ando; Hua Yang; Naohito Nozaki; Tuneko Okazaki; Kinya Yoda
Journal: Mol Cell Biol Date: 2002-04 Impact factor: 4.272

4. Centromere targeting element within the histone fold domain of Cid.

Authors: Danielle Vermaak; Hillary S Hayden; Steven Henikoff
Journal: Mol Cell Biol Date: 2002-11 Impact factor: 4.272

5. The F box protein partner of paired regulates stability of Drosophila centromeric histone H3, CenH3(CID).

Authors: Olga Moreno-Moreno; Sònia Medina-Giró; Mònica Torras-Llort; Fernando Azorín
Journal: Curr Biol Date: 2011-08-25 Impact factor: 10.834

6. Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro.

Authors: K Yoda; S Ando; S Morishita; K Houmura; K Hashimoto; K Takeyasu; T Okazaki
Journal: Proc Natl Acad Sci U S A Date: 2000-06-20 Impact factor: 11.205

7. Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.

Authors: Hao Hu; Yang Liu; Mingzhu Wang; Junnan Fang; Hongda Huang; Na Yang; Yanbo Li; Jianyu Wang; Xuebiao Yao; Yunyu Shi; Guohong Li; Rui-Ming Xu
Journal: Genes Dev Date: 2011-04-08 Impact factor: 11.361

8. Conserved organization of centromeric chromatin in flies and humans.

Authors: Michael D Blower; Beth A Sullivan; Gary H Karpen
Journal: Dev Cell Date: 2002-03 Impact factor: 12.270

9. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3.

Authors: Zheng Zhou; Hanqiao Feng; Bing-Rui Zhou; Rodolfo Ghirlando; Kaifeng Hu; Adam Zwolak; Lisa M Miller Jenkins; Hua Xiao; Nico Tjandra; Carl Wu; Yawen Bai
Journal: Nature Date: 2011-03-16 Impact factor: 49.962

10. Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells.

Authors: Yamini Dalal; Hongda Wang; Stuart Lindsay; Steven Henikoff
Journal: PLoS Biol Date: 2007-08 Impact factor: 8.029

41 in total

1. HJURP uses distinct CENP-A surfaces to recognize and to stabilize CENP-A/histone H4 for centromere assembly.

Authors: Emily A Bassett; Jamie DeNizio; Meghan C Barnhart-Dailey; Tanya Panchenko; Nikolina Sekulic; Danielle J Rogers; Daniel R Foltz; Ben E Black
Journal: Dev Cell Date: 2012-03-08 Impact factor: 12.270

Review 2. Putting CENP-A in its place.

Authors: Madison E Stellfox; Aaron O Bailey; Daniel R Foltz
Journal: Cell Mol Life Sci Date: 2012-06-23 Impact factor: 9.261

Assembly of Drosophila centromeric nucleosomes requires CID dimerization.

1. Crystal structure of the human centromeric nucleosome containing CENP-A.

2. H3.3 is deposited at centromeres in S phase as a placeholder for newly assembled CENP-A in G₁ phase.

3. CENP-A, -B, and -C chromatin complex that contains the I-type alpha-satellite array constitutes the prekinetochore in HeLa cells.

4. Centromere targeting element within the histone fold domain of Cid.

5. The F box protein partner of paired regulates stability of Drosophila centromeric histone H3, CenH3(CID).

6. Human centromere protein A (CENP-A) can replace histone H3 in nucleosome reconstitution in vitro.

7. Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP.

8. Conserved organization of centromeric chromatin in flies and humans.

9. Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3.

10. Tetrameric structure of centromeric nucleosomes in interphase Drosophila cells.

1. HJURP uses distinct CENP-A surfaces to recognize and to stabilize CENP-A/histone H4 for centromere assembly.

Review 2. Putting CENP-A in its place.

Review 3. New insights into nucleosome and chromatin structure: an ordered state or a disordered affair?

4. Solo or doppio: how many CENP-As make a centromeric nucleosome?

Review 5. The centromere: epigenetic control of chromosome segregation during mitosis.

Review 6. Insights into assembly and regulation of centromeric chromatin in Saccharomyces cerevisiae.

7. Cell-cycle-dependent structural transitions in the human CENP-A nucleosome in vivo.

8. CENP-A Ubiquitylation Is Inherited through Dimerization between Cell Divisions.

9. Dimerization of the CENP-A assembly factor HJURP is required for centromeric nucleosome deposition.

10. A two-step mechanism for epigenetic specification of centromere identity and function.