Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water.

A Gram-positive, aerobic, non-motile bacterium designated F3-P9(T), was isolated from dye waste water in Korea and was characterized using a polyphasic taxonomic approach. Comparative 16S rRNA gene sequence analysis showed that strain F3-P9(T) belongs to genus Leucobacter. The 16S rRNA gene sequence similarities among strain F3-P9(T) and validated representatives of the genus Leucobacter ranged from 95.9-97.4%. Strain F3-P9(T) exhibited DNA-DNA relatedness values below 48% with respect to Leucobacter species. The G+C content of the genomic DNA was 67.5 mol%. F3-P9(T) contained MK-11 as the major respiratory quinone. The major fatty acids were anteiso-C(15:0) (48.5%), anteiso-C(17:0) (22.7%), and iso-C(16:0) (14.5%). The peptidoglycan was composed of L-2,4-diaminbutyric acid, alanine, glycine, and glutamic acid. The polar lipid profile showed a major amount of diphosphatidylglycerol (DPG), a moderate amount of phosphatidylglycerol (PG), and two unknown glycolipids. On the basis of its phenotypic and genotypic properties and its phylogenetic distinctiveness, strain F3-P9(T) (KEMC 211-128(T) =KACC 16572(T) =JCM 17539(T)) should be classified in the genus Leucobacter as the type strain of a novel species, for which the name Leucobacter kyeonggiensis sp. nov. is proposed.