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Literature DB >> 22184220

Label-free Raman observation of cytochrome c dynamics during apoptosis.

Masaya Okada¹, Nicholas Isaac Smith, Almar Flotildes Palonpon, Hiromi Endo, Satoshi Kawata, Mikiko Sodeoka, Katsumasa Fujita.

Abstract

We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.

Entities: Gene

Mesh：

Substances：
Solutions
Cytochromes c

Year: 2011 PMID： 22184220 PMCID： PMC3252932 DOI： 10.1073/pnas.1107524108

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

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