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Literature DB >> 24049676

Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy.

Keisuke Isobe¹, Hiroyuki Kawano, Akira Suda, Akiko Kumagai, Atsushi Miyawaki, Katsumi Midorikawa.

Abstract

Imaging of simultaneous two-photon absorption and stimulated Raman scattering is accomplished by detecting the intensity changes of the two-color pulses simultaneously and the mathematical operations of addition and subtraction. The stimulated Raman scattering is quantitatively separated from the two-photon absorption, generated in a mixed solution in which a glycerin solution is miscible in various proportions with a quantum dot solution. Our technique is applied to simultaneous two-photon absorption and stimulated Raman scattering imaging.

Entities: Chemical

Keywords: (180.4315) Nonlinear microscopy; (180.5655) Raman microscopy; (190.4180) Multiphoton processes

Year: 2013 PMID： 24049676 PMCID： PMC3771826 DOI： 10.1364/BOE.4.001548

Source DB: PubMed Journal: Biomed Opt Express ISSN： 2156-7085 Impact factor: 3.732

19 in total

Simultaneous imaging of two-photon absorption and stimulated Raman scattering by spatial overlap modulation nonlinear optical microscopy.

1. Cross-phase modulation imaging.

2. Quantitative chemical imaging with multiplex stimulated Raman scattering microscopy.

3. The phasor approach to fluorescence lifetime imaging analysis.

4. Ultrafast measurement of two-photon absorption by loss modulation.

5. Analysis and experimental assessment of the sensitivity of stimulated Raman scattering microscopy.

6. Scanning coherent anti-Stokes Raman microscope.

7. Two-photon laser scanning fluorescence microscopy.

8. Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy.

9. Background-free deep imaging by spatial overlap modulation nonlinear optical microscopy.

10. Cross-phase modulation spectral shifting: nonlinear phase contrast in a pump-probe microscope.

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