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Literature DB >> 8877377

RNA molecules lighting up under the microscope.

Abstract

RNA in situ hybridization is a useful method for localizing specific mRNAs and studying the spatial and temporal organization of RNA transcription, processing and transport in cells. In this review, I describe methods of RNA in situ hybridization for tissue sections and cell preparations. Special emphasis is placed on the application of non-radioactive-labeled probes for multiparameter cell analysis. In addition, a summary of RNA in situ hybridization studies on RNA transport in the cytoplasm as well as in the nucleus of cells is given.

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Year: 1996 PMID： 8877377 DOI： 10.1007/bf02484398

Source DB: PubMed Journal: Histochem Cell Biol ISSN： 0948-6143 Impact factor: 4.304

98 in total

Review 1. Getting the message from the gene to the synapse: sorting and intracellular transport of RNA in neurons.

Authors: O Steward; G A Banker
Journal: Trends Neurosci Date: 1992-05 Impact factor: 13.837

2. Electron microscopic detection of RNA sequences by non-radioactive in situ hybridization in the mollusk Lymnaea stagnalis.

Authors: R W Dirks; A G Van Dorp; J Van Minnen; J A Fransen; M Van der Ploeg; A K Raap
Journal: J Histochem Cytochem Date: 1992-11 Impact factor: 2.479

3. Simultaneous detection of different mRNA sequences coding for neuropeptide hormones by double in situ hybridization using FITC- and biotin-labeled oligonucleotides.

Authors: R W Dirks; R P van Gijlswijk; R H Tullis; A B Smit; J van Minnen; M van der Ploeg; A K Raap
Journal: J Histochem Cytochem Date: 1990-04 Impact factor: 2.479

4. Bicolor fluorescence in situ hybridization to intron and exon mRNA sequences.

Authors: A K Raap; F M van de Rijke; R W Dirks; C J Sol; R Boom; M van der Ploeg
Journal: Exp Cell Res Date: 1991-12 Impact factor: 3.905

5. In situ hybridization demonstration of poly-adenylated RNA sequences in formalin-fixed paraffin sections using a biotinylated oligonucleotide poly d(T) probe.

Authors: J H Pringle; L Primrose; C N Kind; I C Talbot; I Lauder
Journal: J Pathol Date: 1989-08 Impact factor: 7.996

6. Histological aspects of in situ hybridization. Detection of poly(A) nucleotide sequences in mouse liver sections as a model system.

Authors: A R Jilbert; C J Burrell; E J Gowans; R Rowland
Journal: Histochemistry Date: 1986

Review 7. RNA in the axonal domain: a new dimension in neuronal functioning?

Authors: J Van Minnen
Journal: Histochem J Date: 1994-05

8. Nup145p is required for nuclear export of mRNA and binds homopolymeric RNA in vitro via a novel conserved motif.

Authors: E Fabre; W C Boelens; C Wimmer; I W Mattaj; E C Hurt
Journal: Cell Date: 1994-07-29 Impact factor: 41.582

9. Nucleolar accumulation of poly (A)+ RNA in heat-shocked yeast cells: implication of nucleolar involvement in mRNA transport.

Authors: T Tani; R J Derby; Y Hiraoka; D L Spector
Journal: Mol Biol Cell Date: 1996-01 Impact factor: 4.138

10. Simultaneous use of digoxigenin- and radiolabeled oligodeoxyribonucleotide probes for hybridization histochemistry.

Authors: W S Young
Journal: Neuropeptides Date: 1989 May-Jun Impact factor: 3.286

1 in total

1. CARD In Situ Hybridization: Sights and Signals.

Authors: Ernst J. M. Speel; Paul Komminoth
Journal: Endocr Pathol Date: 1999 Impact factor: 3.943

1 in total