Literature DB >> 16349031

Detection of mRNA in streptomyces cells by whole-cell hybridization with digoxigenin-labeled probes.

D Hahn1, R I Amann, J Zeyer.   

Abstract

Detection of mRNA of the thiostrepton resistance gene (tsr) harbored by plasmid pIJ673 in Streptomyces violacelatus was achieved by whole-cell hybridization with digoxigenin-labeled in vitro transcripts followed by an antibody-alkaline phosphatase detection of the digoxigenin reporter molecule. Prior to hybridization, the cells had to be permeabilized by lysozyme, the detergent Nonidet P-40, and toluene. The permeability of the S. violacelatus cells for probes and the antibody-alkaline phosphatase conjugate was demonstrated by hybridization with digoxigenin-labeled, 16S rRNA-targeted oligonucleotides.

Entities:  

Year:  1993        PMID： 16349031      PMCID： PMC182355          DOI： 10.1128/aem.59.8.2753-2757.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  Molecular and microscopic identification of sulfate-reducing bacteria in multispecies biofilms.

Authors:  R I Amann; J Stromley; R Devereux; R Key; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1992-02       Impact factor: 4.792

2.  Chromosomal in situ hybridization with double-labeled DNA: signal amplification at the probe level.

Authors:  H Scherthan; M Köhler; P Vogt; K von Malsch; D Schweizer
Journal:  Cytogenet Cell Genet       Date:  1992

3.  Genes occupy a fixed and symmetrical position on sister chromatids.

Authors:  M Baumgartner; B Dutrillaux; N Lemieux; A Lilienbaum; D Paulin; E Viegas-Péquignot
Journal:  Cell       Date:  1991-02-22       Impact factor: 41.582

4.  Sequential induction of nodulin gene expression in the developing pea nodule.

Authors:  B Scheres; F van Engelen; E van der Knaap; C van de Wiel; A van Kammen; T Bisseling
Journal:  Plant Cell       Date:  1990-08       Impact factor: 11.277

5.  Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors:  S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

6.  Phylogenetic stains: ribosomal RNA-based probes for the identification of single cells.

Authors:  E F DeLong; G S Wickham; N R Pace
Journal:  Science       Date:  1989-03-10       Impact factor: 47.728

7.  Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.

Authors:  R I Amann; L Krumholz; D A Stahl
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

8.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

9.  pIJ101, a multi-copy broad host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors.

Authors:  T Kieser; D A Hopwood; H M Wright; C J Thompson
Journal:  Mol Gen Genet       Date:  1982

10.  Detection of micro-organisms in soil after in situ hybridization with rRNA-targeted, fluorescently labelled oligonucleotides.

Authors:  D Hahn; R I Amann; W Ludwig; A D Akkermans; K H Schleifer
Journal:  J Gen Microbiol       Date:  1992-05
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  17 in total

1.  Identification of nitrite-oxidizing bacteria with monoclonal antibodies recognizing the nitrite oxidoreductase.

Authors:  S Bartosch; I Wolgast; E Spieck; E Bock
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

2.  Counting and size classification of active soil bacteria by fluorescence in situ hybridization with an rRNA oligonucleotide probe.

Authors:  H Christensen; M Hansen; J Sorensen
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

3.  Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

Authors:  Han Chen; Gomathinayagam Ponniah; Nancy Salonen; Paul Blum
Journal:  Appl Environ Microbiol       Date:  2004-08       Impact factor: 4.792

4.  Locked nucleic acid and flow cytometry-fluorescence in situ hybridization for the detection of bacterial small noncoding RNAs.

Authors:  Kelly L Robertson; Gary J Vora
Journal:  Appl Environ Microbiol       Date:  2011-11-04       Impact factor: 4.792

5.  Improved sensitivity of whole-cell hybridization by the combination of horseradish peroxidase-labeled oligonucleotides and tyramide signal amplification.

Authors:  W Schönhuber; B Fuchs; S Juretschko; R Amann
Journal:  Appl Environ Microbiol       Date:  1997-08       Impact factor: 4.792

6.  Detection of low-copy-number genomic DNA sequences in individual bacterial cells by using peptide nucleic acid-assisted rolling-circle amplification and fluorescence in situ hybridization.

Authors:  Irina Smolina; Charles Lee; Maxim Frank-Kamenetskii
Journal:  Appl Environ Microbiol       Date:  2007-02-09       Impact factor: 4.792

Review 7.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

Authors:  R I Amann; W Ludwig; K H Schleifer
Journal:  Microbiol Rev       Date:  1995-03

Review 8.  Growth kinetics of suspended microbial cells: from single-substrate-controlled growth to mixed-substrate kinetics.

Authors:  K Kovárová-Kovar; T Egli
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 9.  Enumeration of methanogens with a focus on fluorescence in situ hybridization.

Authors:  Sanjay Kumar; Sumit Singh Dagar; Ashok Kumar Mohanty; Sunil Kumar Sirohi; Monica Puniya; Ramesh C Kuhad; K P S Sangu; Gareth Wyn Griffith; Anil Kumar Puniya
Journal:  Naturwissenschaften       Date:  2011-04-08

10.  In situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes.

Authors:  F Chen; J M Gonzalez; W A Dustman; M A Moran; R E Hodson
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792
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