Literature DB >> 22167667

Isotope labeling in insect cells.

Krishna Saxena1, Arpana Dutta, Judith Klein-Seetharaman, Harald Schwalbe.   

Abstract

Recent years have seen remarkable progress in applying nuclear magnetic resonance (NMR) spectroscopy to proteins that have traditionally been difficult to study due to issues with folding, posttranslational modification, and expression levels or combinations thereof. In particular, insect cells have proved useful in allowing large quantities of isotope-labeled, functional proteins to be obtained and purified to homogeneity, allowing study of their structures and dynamics by using NMR. Here, we provide protocols that have proven successful in such endeavors.

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Year:  2012        PMID： 22167667      PMCID： PMC3249615          DOI： 10.1007/978-1-61779-480-3_3

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  47 in total

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Journal:  J Biol Chem       Date:  2003-05-02       Impact factor: 5.157

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Journal:  Nat Rev Mol Cell Biol       Date:  2004-10       Impact factor: 94.444

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Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

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Authors:  A J Dorner; R J Kaufman
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

5.  Molecular and genetic approach to enhancing protein secretion.

Authors:  V Chisholm; C Y Chen; N J Simpson; R A Hitzeman
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

Review 6.  Yeast mutants with increased secretion efficiency.

Authors:  D T Moir
Journal:  Biotechnology       Date:  1989

7.  Isotopic labeling of recombinant proteins from the methylotrophic yeast Pichia pastoris.

Authors:  Andrew R Pickford; Joanne M O'Leary
Journal:  Methods Mol Biol       Date:  2004

8.  Co-expression of molecular chaperones does not improve the heterologous expression of mammalian G-protein coupled receptor expression in yeast.

Authors:  James A Butz; Ronald T Niebauer; Anne Skaja Robinson
Journal:  Biotechnol Bioeng       Date:  2003-11-05       Impact factor: 4.530

9.  Production of human beta interferon in insect cells infected with a baculovirus expression vector.

Authors:  G E Smith; M D Summers; M J Fraser
Journal:  Mol Cell Biol       Date:  1983-12       Impact factor: 4.272

10.  Baculovirus-mediated expression of bacterial genes in dipteran and mammalian cells.

Authors:  L F Carbonell; M J Klowden; L K Miller
Journal:  J Virol       Date:  1985-10       Impact factor: 5.103
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  11 in total

1.  Stable isotope labeling of glycoprotein expressed in silkworms using immunoglobulin G as a test molecule.

Authors:  Hirokazu Yagi; Masatoshi Nakamura; Jun Yokoyama; Ying Zhang; Takumi Yamaguchi; Sachiko Kondo; Jun Kobayashi; Tatsuya Kato; Enoch Y Park; Shiori Nakazawa; Noritaka Hashii; Nana Kawasaki; Koichi Kato
Journal:  J Biomol NMR       Date:  2015-04-23       Impact factor: 2.835

Review 2.  Production of isotope-labeled proteins in insect cells for NMR.

Authors:  Bastian Franke; Christian Opitz; Shin Isogai; Anne Grahl; Leonildo Delgado; Alvar D Gossert; Stephan Grzesiek
Journal:  J Biomol NMR       Date:  2018-04-23       Impact factor: 2.835

3.  Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems.

Authors:  Saeko Yanaka; Hirokazu Yagi; Rina Yogo; Maho Yagi-Utsumi; Koichi Kato
Journal:  J Biomol NMR       Date:  2018-02-28       Impact factor: 2.835

Review 4.  Structural biology of TRP channels.

Authors:  Ute A Hellmich; Rachelle Gaudet
Journal:  Handb Exp Pharmacol       Date:  2014

Review 5.  Permeating disciplines: Overcoming barriers between molecular simulations and classical structure-function approaches in biological ion transport.

Authors:  Rebecca J Howard; Vincenzo Carnevale; Lucie Delemotte; Ute A Hellmich; Brad S Rothberg
Journal:  Biochim Biophys Acta Biomembr       Date:  2017-12-16       Impact factor: 4.019

6.  Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds.

Authors:  Meng Zhang; Xiao-Wei Yu; G V T Swapna; Rong Xiao; Haiyan Zheng; Chong Sha; Yan Xu; Gaetano T Montelione
Journal:  Microb Cell Fact       Date:  2016-07-13       Impact factor: 5.328

7.  Preparation of highly and generally enriched mammalian tissues for solid state NMR.

Authors:  Veronica Wai Ching Wong; David G Reid; Wing Ying Chow; Rakesh Rajan; Maggie Green; Roger A Brooks; Melinda J Duer
Journal:  J Biomol NMR       Date:  2015-09-25       Impact factor: 2.835

8.  Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning.

Authors:  Evgeny Nimerovsky; Kumar Tekwani Movellan; Xizhou Cecily Zhang; Marcel C Forster; Eszter Najbauer; Kai Xue; Rıza Dervişoǧlu; Karin Giller; Christian Griesinger; Stefan Becker; Loren B Andreas
Journal:  Biomolecules       Date:  2021-05-18

9.  Solid state NMR of isotope labelled murine fur: a powerful tool to study atomic level keratin structure and treatment effects.

Authors:  Wai Ching Veronica Wong; Aurimas Narkevicius; Wing Ying Chow; David G Reid; Rakesh Rajan; Roger A Brooks; Maggie Green; Melinda J Duer
Journal:  J Biomol NMR       Date:  2016-10-03       Impact factor: 2.835

10.  Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling.

Authors:  Hirokazu Yagi; Saeko Yanaka; Rina Yogo; Akari Ikeda; Masayoshi Onitsuka; Toshio Yamazaki; Tatsuya Kato; Enoch Y Park; Jun Yokoyama; Koichi Kato
Journal:  Biomolecules       Date:  2020-10-26
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