Literature DB >> 22113890

Characterization of membrane proteins in isolated native cellular membranes by dynamic nuclear polarization solid-state NMR spectroscopy without purification and reconstitution.

Tomas Jacso1, W Trent Franks, Honor Rose, Uwe Fink, Jana Broecker, Sandro Keller, Hartmut Oschkinat, Bernd Reif.   

Abstract

Membrane proteins in their native cellular membranes are accessible by dynamic nuclear polarization magic angle spinning solid-state NMR spectroscopy without the need of purification and reconstitution (see picture). Dynamic nuclear polarization is essential to achieve the required gain in sensitivity to observe the membrane protein of interest.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2011        PMID： 22113890     DOI： 10.1002/anie.201104987

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  42 in total

1.  Enlightening the photoactive site of channelrhodopsin-2 by DNP-enhanced solid-state NMR spectroscopy.

Authors:  Johanna Becker-Baldus; Christian Bamann; Krishna Saxena; Henrik Gustmann; Lynda J Brown; Richard C D Brown; Christian Reiter; Ernst Bamberg; Josef Wachtveitl; Harald Schwalbe; Clemens Glaubitz
Journal:  Proc Natl Acad Sci U S A       Date:  2015-07-27       Impact factor: 11.205

Review 2.  In-Cell Solid-State NMR: An Emerging Technique for the Study of Biological Membranes.

Authors:  Xavier L Warnet; Alexandre A Arnold; Isabelle Marcotte; Dror E Warschawski
Journal:  Biophys J       Date:  2015-12-15       Impact factor: 4.033

Review 3.  Helical membrane protein conformations and their environment.

Authors:  Timothy A Cross; Dylan T Murray; Anthony Watts
Journal:  Eur Biophys J       Date:  2013-09-01       Impact factor: 1.733

4.  Cryogenic solid state NMR studies of fibrils of the Alzheimer's disease amyloid-β peptide: perspectives for DNP.

Authors:  Juan-Miguel Lopez del Amo; Dennis Schneider; Antoine Loquet; Adam Lange; Bernd Reif
Journal:  J Biomol NMR       Date:  2013-06-22       Impact factor: 2.835

Review 5.  Membrane proteins in their native habitat as seen by solid-state NMR spectroscopy.

Authors:  Leonid S Brown; Vladimir Ladizhansky
Journal:  Protein Sci       Date:  2015-05-27       Impact factor: 6.725

6.  A solid view of membrane proteins in situ.

Authors:  Marc Baldus
Journal:  Biophys J       Date:  2015-04-07       Impact factor: 4.033

7.  A versatile and modular quasi optics-based 200GHz dual dynamic nuclear polarization and electron paramagnetic resonance instrument.

Authors:  Ting Ann Siaw; Alisa Leavesley; Alicia Lund; Ilia Kaminker; Songi Han
Journal:  J Magn Reson       Date:  2016-03       Impact factor: 2.229

8.  M2 proton channel structural validation from full-length protein samples in synthetic bilayers and E. coli membranes.

Authors:  Yimin Miao; Huajun Qin; Riqiang Fu; Mukesh Sharma; Thach V Can; Ivan Hung; Sorin Luca; Peter L Gor'kov; William W Brey; Timothy A Cross
Journal:  Angew Chem Int Ed Engl       Date:  2012-07-13       Impact factor: 15.336

9.  Dynamics in the solid-state: perspectives for the investigation of amyloid aggregates, membrane proteins and soluble protein complexes.

Authors:  Rasmus Linser; Riddhiman Sarkar; Alexey Krushelnitzky; Andi Mainz; Bernd Reif
Journal:  J Biomol NMR       Date:  2014-03-05       Impact factor: 2.835

10.  Perturbation of nuclear spin polarizations in solid state NMR of nitroxide-doped samples by magic-angle spinning without microwaves.

Authors:  Kent R Thurber; Robert Tycko
Journal:  J Chem Phys       Date:  2014-05-14       Impact factor: 3.488
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