Literature DB >> 22102238

Crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase 2 from Arabidopsis thaliana.

Michael D W Griffin1, Jagan M Billakanti, Juliet A Gerrard, Renwick C J Dobson, F Grant Pearce.   

Abstract

Dihydrodipicolinate synthase (DHDPS; EC 4.2.1.52) catalyzes the first committed step of the lysine-biosynthetic pathway in plants and bacteria. Since (S)-lysine biosynthesis does not occur in animals, DHDPS is an attractive target for rational antibiotic and herbicide design. Here, the cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DHDPS2 from Arabidopsis thaliana are reported. Diffraction-quality protein crystals belonged to space group P2(1)2(1)2.
© 2011 International Union of Crystallography. All rights reserved.

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Year:  2011        PMID： 22102238      PMCID： PMC3212457          DOI： 10.1107/S1744309111033276

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  41 in total

1.  ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites.

Authors:  O Emanuelsson; H Nielsen; G von Heijne
Journal:  Protein Sci       Date:  1999-05       Impact factor: 6.725

2.  Towards rationalization of crystallization screening for small- to medium-sized academic laboratories: the PACT/JCSG+ strategy.

Authors:  Janet Newman; David Egan; Thomas S Walter; Ran Meged; Ian Berry; Marouane Ben Jelloul; Joel L Sussman; David I Stuart; Anastassis Perrakis
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-09-28

3.  Federated repositories of X-ray diffraction images.

Authors:  Steve Androulakis; Jason Schmidberger; Mark A Bate; Ross DeGori; Anthony Beitz; Cyrus Keong; Bob Cameron; Sheena McGowan; Corrine J Porter; Andrew Harrison; Jane Hunter; Jennifer L Martin; Bostjan Kobe; Renwick C J Dobson; Michael W Parker; James C Whisstock; Joan Gray; Andrew Treloar; David Groenewegen; Neil Dickson; Ashley M Buckle
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2008-06-18

4.  The crystal structures of native and (S)-lysine-bound dihydrodipicolinate synthase from Escherichia coli with improved resolution show new features of biological significance.

Authors:  Renwick C J Dobson; Michael D W Griffin; Geoffrey B Jameson; Juliet A Gerrard
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-07-20

5.  Cloning and characterisation of dihydrodipicolinate synthase from the pathogen Neisseria meningitidis.

Authors:  Sean R A Devenish; Frances H A Huisman; Emily J Parker; Andrea T Hadfield; Juliet A Gerrard
Journal:  Biochim Biophys Acta       Date:  2009-02-21

6.  Conformationally constrained diketopimelic acid analogues as inhibitors of dihydrodipicolinate synthase.

Authors:  Berin A Boughton; Renwick C J Dobson; Juliet A Gerrard; Craig A Hutton
Journal:  Bioorg Med Chem Lett       Date:  2007-12-03       Impact factor: 2.823

7.  Structural, functional and calorimetric investigation of MosA, a dihydrodipicolinate synthase from Sinorhizobium meliloti l5-30, does not support involvement in rhizopine biosynthesis.

Authors:  Christopher P Phenix; Kurt Nienaber; Pui Hang Tam; Louis T J Delbaere; David R J Palmer
Journal:  Chembiochem       Date:  2008-07-02       Impact factor: 3.164

8.  Crystallization and preliminary X-ray analysis of dihydrodipicolinate synthase from Clostridium botulinum in the presence of its substrate pyruvate.

Authors:  Sarah C Atkinson; Renwick C J Dobson; Janet M Newman; Michael A Gorman; Con Dogovski; Michael W Parker; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-02-14

9.  Expression, purification, crystallization and preliminary X-ray diffraction analysis of dihydrodipicolinate synthase from Bacillus anthracis in the presence of pyruvate.

Authors:  Jarrod E Voss; Stephen W Scally; Nicole L Taylor; Con Dogovski; Malcolm R Alderton; Craig A Hutton; Juliet A Gerrard; Michael W Parker; Renwick C J Dobson; Matthew A Perugini
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2009-01-31

Review 10.  Scaling and assessment of data quality.

Authors:  Philip Evans
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2005-12-14
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  1 in total

1.  Characterisation of the first enzymes committed to lysine biosynthesis in Arabidopsis thaliana.

Authors:  Michael D W Griffin; Jagan M Billakanti; Akshita Wason; Sabrina Keller; Haydyn D T Mertens; Sarah C Atkinson; Renwick C J Dobson; Matthew A Perugini; Juliet A Gerrard; Frederick Grant Pearce
Journal:  PLoS One       Date:  2012-07-05       Impact factor: 3.240
  1 in total

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