Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes.

PURPOSE: Cationic liposomes (CLs) are composed of phospholipid bilayers. One of the most important applications of these particles is in drug and gene delivery. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. The aim of this study was to develop novel CLs containing magnetite to overcome the deficiencies.
MATERIALS AND METHODS: CLs and magnetic cationic liposomes (MCLs) were prepared using the freeze-dried empty liposome method. Luciferase-harboring vectors (pGL3) were transferred into liposomes and the transfection efficiencies were determined by luciferase assay. Firefly luciferase is one of most popular reporter genes often used to measure the efficiency of gene transfer in vivo and in vitro. Different formulations of liposomes have been used for delivery of different kinds of gene reporters. Lipoplex (liposome-plasmid DNA complexes) formation was monitored by gel retardation assay. Size and charge of lipoplexes were determined using particle size analysis. Chinese hamster ovary cells were transfected by lipoplexes (liposome-pGL3); transfection efficiency and gene expression level was evaluated by luciferase assay.
RESULTS: High transfection efficiency of plasmid by CLs and novel nanomagnetic CLs was achieved. Moreover, lipoplexes showed less cytotoxicity than polyethyleneimine and Lipofectamine.
CONCLUSION: Novel liposome compositions (1,2-dipalmitoyl-sn-glycero-3-phosphocholine [DPPC]/dioctadecyldimethylammonium bromide [DOAB] and DPPC/cholesterol/DOAB) with high transfection efficiency can be useful in gene delivery in vitro. MCLs can also be used for targeted gene delivery, due to magnetic characteristic for conduction of genes or drugs to target organs.

Introduction

Nanoliposomes are self-closed colloidal particles in which bilayered membrane(s) composed of self-aggregated n class="Chemical">lipid molecules make the vesicles. They encapsulate a fractionpan> of the medium in which they are suspended into their interior.1

Liposomal vesicles have drawn the attention of researchers as potential carriers of various bioactive molecules that could be used for therapeutic applications in both n class="Species">humans anpan>d anpan>imals.2,3 Liposomes have been studied as models of biological membranpan>es anpan>d more recently as carriers for the introduction of genes and drugs into target cells;1,4–7 thus, liposomes have been successful as carriers of antitumor drugs in cancer chemotherapy8 and for gene delivery purposes.9 Recent work has shown that nucleic acids can be entrapped in cationic liposomes (CLs) and subsequently transfected into cultured mammalian cells, where they can express the information they carry.10 CLs represent one of the most widespread nonviral transfection systems for gene delivery.11 CLs are usually employed as a gene delivery system because of their low toxicity, low immunogenicity, ease of preparation,12 size-independent delivery of nucleic acids, and quality control and capacity for mass production at reasonable cost.13–15 Different methods for increasing liposome performance have been studied and have focused on the manufacturing of surface proteins or a combination of targeting ligands such as antibodies,16 transferin, and lactose. However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, including low transfection efficiency in vivo. With regard to this, novel CLs containing magnetite (MAG) were developed in this study to overcome the deficiencies. Magnetic cationic liposomes (MCLs) are prepared by incorporating MAG into CLs. MAG force-mediated gene delivery involves the use of a static magnetic field that guides magnetic particle-associated gene vectors to accumulate on the cell surface.17 High MAG concentration increases the size of CLs/plasmid DNA (pDNA) and MCLs/pDNA complex. The transfection efficiency of Chinese hamster ovary (CHO) cell line was investigated by using luciferase as a reporter.

On the other hand, genetically encoded in class="Chemical">maginpan>g reporters inpan>troduced inpan>to cells anpan>d tranpan>sgenic anpan>imals enable nonpan>inpan>vasive, lonpan>gitudinpan>al studies of dynamic biological processes inpan> vivo. The most commonpan> reporters inpan>clude a firefly luciferase; the pan> class="Species">North American firefly Photinus pyralis luciferase, which emits yellow-green light, has been adapted for a variety of applications. Luciferase variants with red-shifted bioluminescence can be used alone for in vivo imaging. As transmission efficiency of light through tissue increases greatly for wavelengths above 600 nm, red-shifted firefly luciferase (λmax = 615 nm) could be successfully employed as a sensitive reporter in mammalian cells. A number of point mutations have previously been identified that significantly produce red-emitting firefly luciferase.18

In this study, the freeze-dried empty liposome (FDEL) method19 was used to prepare different formulations of n class="Chemical">CLs anpan>d MCLs. The complex of liposome and plasmid (lipoplex) was formed in different solvents. A mutant pGL3 plasmid with red-emitting P. pyralis luciferase gene with bioluminescence properties suitable for in vivo imaging was prepared. Size and charge of the lipolexes (CLs, MCLs, CLs/pDNA, MCLs/pDNA) were analyzed and related to transfection efficiency.

Materials and methods

Chemicals

The neutral n class="Chemical">lipid pan> class="Chemical">1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) was purchased from Avanti Polar Lipids, Inc (Alabaster, AL). Cholesterol (Chol) was purchased from Sigma Aldrich (St Louis, MO). Dr Mahmoud Reza Jafari (Mashhad University of Medical Sciences, Mashad, Iran) kindly provided the dioctadecyldimethylammonium bromide (DOAB). Both D-luciferin potassium salt and adenosine-5′-triphosphate (ATP) were obtained from SynChem, Inc (Elk Grove Village, IL). Both MTT 3(4,5dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and magnetic nanoparticles (Fe3O4) were purchased from Sigma-Aldrich. All other chemicals were of commercial analytical grade and were used without further purification.

Production of red-emitting P. pyralis luciferase gene by site-directed mutagenesis

The QuikChange™ Site-Directed Mutagenesis Kit ( Stratagene, La Jolla, CA) was used to create red-emitting n class="Species">P. pyralis with conpan>versionpan> of pan> class="Mutation">S284 to T (S284T). The native gene had been cloned into a pGL3-control vector. The plasmids containing the mutant luciferase (S284T) were amplified using PrimeStar® DNA Polymerase (Takara Bio Inc, Otsu, Shiga, Japan) and two complementary primers containing the desired mutation, using a thermal cycler (PTC-1148, Bio-Rad, Singapore) (one cycle at 95°C; 20 cycles at 95°C for 1 minute, 55°C for 1 minute, and 68°C for 13 minutes; and a final extension for 10 minutes at 68°C). Subsequently, amplified products were purified using a clean-up kit ( Bioneer, Alameda, CA). The products were treated with DpnI to digest the non-mutated parental plasmids and were then transformed into competent Escherichia coli XL1-Blue cells. The primers and the corresponding reverse complements used were P. pyralis pGL3-control vector as a template and the primer set for S284T (where bold represents the mutated codon): 5′-CAGGATTACAAGATTCAAACTGCGCTGCT GGTG-3′ (forward); 5′-CACCAGCAGCGCAGTTTGAAT CTTGTAATCCTG-3 (reverse).

Preparation of CLs

Nanoliposomes were prepared in three formulations: (1) n class="Chemical">DPPC/n class="Chemical">Chol liposomes, (2) DPPC/DOAB liposomes, and (3) DPPC/Chol/DOAB liposomes.

The formulation for the n class="Chemical">DPPC/pan> class="Chemical">Chol liposomes was prepared by the FDEL method.19 The Chol and neutral lipid (DPPC) powders were mixed at a molar ratio of 1:5 and were dissolved in the organic phase (chloroform). The chloroform was evaporated using a rotary evaporator (Heidolph, Schwabach, Germany) under reduced pressure (temperature 37°C, under vacuum, and 40 rpm) and lipid thin film was obtained. The lipid film was incubated at room temperature for 24 hours to form dried film. At the next step, distilled water at 50°C was added to the dried lipid film and vortex-mixed vigorously for 30 minutes to obtain dispersion. Primary homogenization was performed by bath sonicator (Soltec, Milan, Italy) for 20 minutes and was then sonicated by microtip probe sonicators (Dr Hielscher, Teltow, Germany), at pulse on for 5.0 seconds and pulse off for 10.0 seconds, for 3 minutes. The final step was lyophilization of homogeneous suspension by lyophilizer (VDH-2040, Snijders Scientific BV, Tilburg, The Netherlands). In this step, the suspension was frozen in liquid nitrogen, and then dried in a lyophilizer at −40°C and vacuum (0.4 millibars) condition. The product powder of vesicles was kept at −20°C until used.1,20

For the n class="Chemical">DPPC/DOAB liposomes, the n class="Chemical">DPPC and DOAB were mixed at a molar ratio of 1:1 and were dissolved in chloroform solution. The rest of the procedure was the same as for the DPPC/Chol liposomes.

For the n class="Chemical">DPPC/Chol/DOAB liposomes, the n class="Chemical">DPPC, Chol, and DOAB were mixed at a molar ratio of 7:2:1 and were dissolved in chloroform solution. The rest of the procedure was the same as for the DPPC/Chol liposomes.

Preparation of CL/pDNA complexes

Three methods were used for preparing liposomes to entrap plasmids: (1) suspension in n class="Chemical">water, (2) suspensionpan> inpan> pan> class="Chemical">ethanol, and (3) suspension in plasmid aqueous buffer. In the first and second procedures, plasmids were mixed with liposomes in water or ethanol and pre-incubated at 4°C for 24 hours. The results of transfection efficiency by these liposomes were compared.

Preparation of MCL and MCL/pDNA complexes

n class="Chemical">DPPC, pan> class="Chemical">DOAB, and Chol were used as liposome components and MAG was used as the core. The DPPC, Chol, and DOAB, mixed at a molar ratio of 7:2:1, were dissolved in chloroform with different concentrations of MAG (0.5 and 1 mg/mL) in the presence of 5% dextrose solution 2.5 mL. The resulting suspension was emulsified by sonication. The organic phase was then evaporated at 25°C using a rotary evaporator under reduced pressure, and 5% dextrose solution 2.5 mL was added to the mixture of lipid and MAG colloid. The suspension was sonicated for 15 minutes and then centrifuged at 1000× g for 15 minutes, to precipitate unincorporated MAG and retain the MCLs,21 which were then stored at 4°C. The MCL/ pDNA complex was prepared by mixing pDNA and MCL in various ratios in serum-free Dulbecco’s Modified Eagle Medium (DMEM; made from GIBCO® powder 12800-116 [Stem Cell Technology Research Center, Royan Institute for Stem Cell Biology and Techology Iran]), and the mixture was incubated at room temperature for 45 minutes. This final composition was ready for performing transfection procedures according to a reported method.22

Determination of zeta potential, particle size, and polydispersity index of liposomes

Values of the zeta potential of liposomes indirecn class="Chemical">tly reflect vesicle surface net charge anpan>d canpan> therefore be used to evaluate the extent of inpan>teractionpan> of the liposomal surface cationpan>ic charges with the anpan>ionpan>ic charges of DNA. The average particle size anpan>d the polydispersity of the particle-size distributionpan> of the liposomes were determinpan>ed by dynamic light scatterinpan>g usinpan>g photonpan> correlationpan> spectroscopy. The measurements were performed at 25°C usinpan>g a Zetasizer Nanpan>o ZS inpan>strument (Malvernpan> Inpan>struments Ltd, Malvernpan>, Worcestershire, UK) equipped with a pan> class="Chemical">helium-neon laser and a scattering angle of 173°. Furthermore, the zeta potential of the liposome dispersions was also measured with the same instrument at 25°C by the electrophoretic mobility. All samples were not further diluted for each particle size and zeta potential measurement. A typical liposome refractive index of 1.45 was used.

Gel retardation analysis of lipoplexes

Various formulations of liposome (n class="Chemical">DPPC/pan> class="Chemical">Chol, DPPC/ Chol/DOAB, and DPPC/DOAB) at 5:1, 7:2:1, and 1:1, respectively, molar ratios were mixed with DNA (0.5 mg) by three methods (suspension in water, suspension in ethanol, and suspension in plasmid aqueous buffer) at an N/P ratio of 2.5 and then incubated at room temperature for 45 minutes. Lipoplexes were run on 0.7% agarose gel and were visualized by ultraviolet illumination (uv-tech, Kiel, Germany) and electrophoretic mobility was investigated to evaluate the quantity of DNA entrapment. Empty liposomes and naked plasmid were used as controls.

Cell lines and culture

n class="Species">Chinese hamster ovarian cell line was a gift from Dr Baharvand (Royan Inpan>stitute, Tehran, Iran). n class="CellLine">CHO cells were cultured in high-glucose DMEM supplemented with 10% fetal bovine serum (FBS) (GIBCO®EU 10270), penicillin 100 U/mL and streptomycin 100 μg/mL in a humidified atmosphere of 5% CO2 and 37°C. Cells were seeded in a 24-well cell culture plate 1 day before transfection.23

Cell viability assay

n class="CellLine">CHO cells were seeded in 96-well plates and incubated for 24 hours. Cells were attached to the plate surface and were then treated with n class="Chemical">CLs and MCLs at the same concentrations used for transfection experiments. Moreover, polyethyleneimine (PEI) 0.6 μL (1.9 μg/mL) and Lipofectamine 1.0 μL (1 mg/mL) per well were used. Cell viability was assayed using MTT according to a reported method24 with minor modifications. Briefly, MTT 10 μL (5 mg/mL) was added to each well and the cells were incubated at 37°C for 4 hours. The formazan product was dissolved in 10% sodium dodecyl sulfate 100 μL containing hydrochloric acid 15 mM.25 Color intensity was measured using an absorbance microplate reader (ELx800™, BioTek, Winooski, VT) at test and reference wavelengths of 570 nm.

In vitro transfection experiment

One day before transfection, n class="CellLine">CHO cells were seeded inpan> 24-well sterile culture plates at a density of 2 × 105 cells/well anpan>d were grownpan> overnpan>ight to approximately 80% conpan>fluence. For tranpan>sfectionpan>, the growth medium was removed anpan>d then cells were washed twice with pre-warmed pan> class="Chemical">phosphate-buffered saline (PBS). The complexes with three different combinations of liposomes (DPPC/Chol, DPPC/ DOAB, and DPPC/Chol/DOAB, with an N/P ratio of 2.5), and Lipofectamine™ and PEI as controls, were, in serum-free DMEM, added to the cells (pDNA 0.5 μg/well) and the cells were then incubated for 6 hours. In addition, the complexes with different N/P ratios (2.5 and 5) and two different MAG concentrations (0.5 and 1 mg/mL) were, in serum-free DMEM, added to the wells (pDNA 0.5 μg/well) and the cells were incubated for 4 and 6 hours. After transfection, the incubation medium was replaced with DMEM supplemented with 10% FBS and incubated for another 24 hours (in a humidified atmosphere of 5% CO2 and 37°C) prior to evaluation of transfection efficiency using luciferase assay. In the case of magnetic induction MCLs/ pDNA lipoplexes and CLs/pDNA complexes, a permanent magnet of strength 0.3 T was placed under the cell culture plate. The magnet was removed at 30 and 60 minutes after addition of transfection suspension (MCLs/pDNA lipoplexes and CLs/pDNA complexes) and the incubation continued for 4 and 6 hours. To analyze luciferase activity, the transfected cells in each well were washed twice gently with PBS 3 mL and the cells were lysed with cell culture lysis buffer 100 μL (Promega Corporation, Madison, WI). The luciferase activity in each sample was indicated as the relative light unit in presence of luciferin, ATP, and magnesium2+ as the substrates of luciferase with a luminometer (Berthold Detection Systems GmbH, Pforzheim, Germany).

Results and discussion

Zeta potential, particle size, and polydispersity index of liposomes

Many physicochemical factors influence CL transfection efficiency including the charge of n class="Chemical">CLs, the size of the complexes, anpan>d the total pan> class="Chemical">lipid/pDNA ratio. These parameters can affect stability and reproducibility of used liposomes in transfection.26 Since the use of magnetic particles changes the charge and size of CLs, the amount of MAG incorporated into liposomes should be low enough to not cause a drastic change in charge and size and high enough to respond to an external magnetic field.27 The mean particle size and zeta potential (surface charge potential) of MCLs with two different concentrations of MAG (0.5 and 1.0 mg/mL) and CLs were measured. As seen in Table 1, the average size of particles in MCLs and MCLs/pDNA was higher than in CLs and CLs/pDNA complexes. The MCLs with a lower concentration of MAG (0.5 mg/mL) were smaller than those with higher concentration (1.0 mg/mL). On the other hand, the increasing average particle size of MCLs/pDNA and CLs/pDNA compared with MCLs and CLs could be due to adhesion and fusion of plasmid to liposomes.28 Zeta potential of MCLs and MCLs/pDNA decreased significantly compared with CLs and CLs/pDNA. The addition of MAG 0.5 mg/mL resulted in the highest decrease in zeta potential of MCLs and MCLs/pDNA complexes compared with those without MAG (CLs and CLs/pDNA complexes). The zeta potential of liposomes and complexes containing MAG 0.5 mg/mL was not significantly different from the zeta potential of those containing MAG 1.0 mg/mL. Therefore, it may be suggested that MAG interaction with the cationic polar head groups of the bilayer in CLs neutralize some of the positive surface charge characteristics through charge shielding.

Table 1

The mean particle size and zeta potential of cationic liposomes (CLs) and magnetic cationic liposomes (MCLs) with two different concentrations of magnetite (MAG; 0.5 and 1 mg/mL) and their complexes with plasmid DNA (pDNA)

Group	Sample	Mean particle size (nm)	pdl	Zeta potential (mV)
1	CLs	72.82	0.2	+46
2	MCLs (MAG 0.5 mg/mL)	86.63	0.2	+44.1
3	MCLs (MAG 1 mg/mL)	295.7	0.3	+51.9
4	CLs/pDNA complexes	135	0.3	+20
5	MCLs/pDNA complexes (MAG 0.5 mg/mL)	132.7	0.2	+17.2
6	MCLs/pDNA complexes (MAG 1 mg/mL)	372.5	0.2	+33.5

Abbreviation: pdl, polydispersity index.

Three types of n class="Chemical">CLs with different molar ratios (pan> class="Chemical">DPPC/Chol, 5:1; DPPC/DOAB, 1:1; DPPC/Chol/DOAB, 7:2:1) were prepared. Lipoplexes of these compositions were prepared by three methods (suspension in water, suspension in ethanol, and suspension in plasmid aqueous buffer). Gel retardation analysis of lipoplexes showed the ability of different formulations of liposome to entrap pDNA. With the first composition (DPPC/ Chol; molar ratio 5:1), it seemed that because of the absence of cationic lipid with a positive charge, they had no ability to entrap plasmid and thus the plasmid moved through the gel (Figure 1A). With the second liposome composition (DPPC/ DOAB; molar ratio 1:1) having cationic lipid, DNA retardation suggested interaction between negatively charge DNA and CLs (Figure 1B). In this case, preparation of liposomes using an aqueous solution of plasmid could not entrap the plasmid, as observed in the third lane. It seems that suspending of liposome powder 1 day before mixing with pDNA and formation of lipoplexes is necessary for pDNA entrapment. The third complex (DPPC/Chol/DOAB; molar ratio 7:2:1), which comprised a neutral phospholipid, Chol, and a cationic lipid, was prepared with all three methods (water, ethanol, aqueous solution of plasmid) (Figure 1C) and was found to be efficient in DNA entrapment except in the aqueous solution of plasmid sample. The MCLs/pDNA lipoplex (DPPC/Chol/ DOAB; molar ratio 7:2:1) in two concentrations of MAG (0.5 and 1 mg/mL) was examined (Figure 1D). This combination was also found to be effective in DNA entrapment, presumably because of the presence of cationic lipids.

Figure 1

Gel retardation of (A) 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC)/cholesterol (Chol) at a molar ratio of 5:1 lipoplex; (B) DPPC/ dioctadecyldimethylammonium bromide (DOAB) at a molar ratio of 1:1 lipoplex; (C) DPPC/Chol/ DOAB at a molar ratio of 7:2:1 lipoplex; (D) magnetic cationic liposomes/pDNA with different concentrations of magnetite (MAG). pGL3 plasmid and empty liposomes were used as controls. For all panels, (1) is suspension in water, (2) is suspension in ethanol, and (3) is suspension in aqueous solution of plasmid.

Optimization of CLs composition

Liposomes with composition of n class="Chemical">DPPC/Chol/DOAB at molar ratios of 7:2:1, 5:2:1, anpan>d 7:4:1 were prepared inpan> pan> class="Chemical">water suspension. The transfection efficiencies of these three compounds were compared. Results of enzymatic assay indicated that transfection efficiency was highest in liposomes that contain a higher ratio of cationic lipid to the total lipid content (CL/TL) of liposomes (DPPC/Chol/DOAB at a molar ratio of 5:2:1 with CL/TL = 1:8, in comparison with similar liposome composition at a molar ratio of 7:2:1 with CL/TL = 1:10, and similar liposome composition at a molar ratio of 7:4:1 with CL/TL = 1:12). So with decrease in ratio of CL/TL content of liposomes, reduction of transfection efficiency was observed (Figure 2).

Figure 2

Comparing the transfection efficiencies of 1,2-dipalmitoyl-sn-glycero-3- phosphocholine (DPPC)/cholesterol (Chol)/dioctadecyldimethylammonium bromide (DOAB) and DPPC/DOAB liposomes with different molar ratios.

Abbreviation: RLU, relative light unit.

In addition, preparation of other liposomes with n class="Chemical">DPPC/ pan> class="Chemical">DOAB at a molar ratio of 1:1 and 3:1 in water suspension was compared. Similarly, enzymatic assay indicated that transfection efficiency was higher in liposomes containing a higher ratio of cationic lipid to the total lipid structure of liposomes (Figure 2).

However, based on a simple comparison of transfection efficiencies between two liposome preparations (n class="Chemical">DPPC/ pan> class="Chemical">DOAB and DPPC/Chol/DOAB), the authors expected higher transfection efficiency of DPPC/DOAB than DPPC/ Chol/DOAB because of a higher ratio of CL/TL (1:2, 1:4 for DPPC/DOAB, respectively; 1:8,1:10,1:12 for DPPC/ Chol/DOAB, respectively). Because of the important role of Chol in the formation and stability of the liposomal membrane and because more types of lipids participated in DPPC/Chol/DOAB liposomes than in DPPC/DOAB liposomes, the transfection efficiency of DPPC/Chol/ DOAB was higher. Similar results have been reported in previous studies.20

Three preparation methods (suspension in n class="Chemical">ethanol, suspensionpan> inpan> pan> class="Chemical">water, and suspension in plasmid aqueous buffer) were used before plasmid entrapment. Preparation in water and ethanol was accompanied with full loading of pDNA, while suspension in plasmid aqueous buffer did not bring any entrapment (Figure 1B and C). Suspension in ethanol had higher transfection efficiency than suspension in water (Figure 3); therefore, it may be surmised that higher transfection efficiency in ethanol was due to the presence of ethanol. The positive effect of ethanol on lipid-mediated transfection has been reported previously.29

Figure 3

Comparison of luciferase assay in cells transfected by 1,2-dipalmitoyl-snglycero-3-phosphocholine (DPPC)/cholesterol (Chol)/dioctadecyldimethylammonium bromide (DOAB) liposomes composition at a molar ratio of 7:2:1 and DPPC/DOAB at a molar ratio of 1:1, and comparison of different methods of preparing them.

Abbreviation: RLU, relative light unit.

Transfection efficiencies of CLs with different compositions

n class="CellLine">CHO cells were transfected with n class="Chemical">DPPC/DOAB (molar ratio 1:1) and DPPC/Chol/DOAB (molar ratio 7:2:1) and different preparation methods were compared. The luciferase activity of cell extracts, as the indicator of transfection efficiency, was determined. As indicated in Figure 3, the highest transfection efficiency was obtained for liposomes with DPP/Chol/ DOAB composition in ethanol suspension at a molar ratio of 7:2:1.

Optimization of MCLs/pDNA N/P ratio

The effects of different concentrations of n class="Chemical">MAG (0.5 and 1.0 mg/mL) and different ratios of pDNA and n class="Disease">MCLs (N/P ratio) on transfection efficiency were studied. The incubation time for transfection was 6 hours. Higher luciferase activity (highest transfection efficiency) was obtained for MCLs/ pDNA (N/P = 5) with MAG concentration of 0.5 mg/mL (Figure 4).

Figure 4

Optimizing the magnetic cationic liposome (MCL)/plasmid DNA (pDNA) N/P ratio evaluated by luciferase assay in Chinese hamster ovary cells.

Abbreviation: RLU, relative light unit.

Optimization of incubation time

The effects of incubation time on transfection efficiency of n class="Disease">MCLs with two n class="Chemical">MAG concentrations (0.5 and 1.0 mg/mL) and its effect on transfection efficiency of MCLs/pDNA lipoplexes with the optimal combination ratio were investigated. For MCLs/pDNA lipoplex in which the MCL MAG concentration was 0.5 mg/mL, maximal luciferase activity was shown at 6 hours’ incubation time (Figure 5). As indicated in Figure 6, sufficient transfection was observed upon 4 hours’ incubation with MCLs, an effect that was not observed for CLs. Therefore, the optimum time of liposome incubation was reduced in the presence of MCLs.

Figure 5

Optimization of incubation time evaluated by luciferase assay in Chinese hamster ovary cells with magnetic induction.

Abbreviations: MCL, magnetic cationic liposome; RLU, relative light unit.

Figure 6

Comparing the nonmagnetic induction transfection efficiencies of lipoplexes under the optimal transfection conditions (6-hour incubation time) in Chinese hamster ovary cells.

Abbreviations: CL, cationic liposome; MCL, magnetic cationic liposome; RLU, relative light unit.

Transfection efficiency of MCLs

Many factors influence the transfection efficiency of n class="Chemical">CLs, inpan>cludinpan>g type of cell tranpan>sfected, pDNA/pan> class="Chemical">CLs ratio, liposome concentration, and incubation time.30 Long incubation time may increase the cytotoxicity of liposome, so optimization of CLs/plasmid ratio and also reducing the incubation time could improve conditions of transfection by CLs. Using MCLs and an external magnetic field can decrease cytotoxicity, because of reduced incubation time. The MCLs/pDNA with MAG 0.5 mg/mL complex was more effective in transfection because of its smaller size and lower zeta potential compare with MCLs/pDNA with MAG 1.0 mg/mL complex (Figure 4).

Comparison of transfection efficiency under optimal transfection conditions in CHO cells

Transfection efficiency of lipoplexes including n class="Chemical">CLs/pDNA anpan>d MCLs/pDNA (with two different concentrations of MAG) was compared by luciferase assay using the optimal N/P ratio and incubation time (Figure 6). The luciferase activity in the transfected cells by CLs/pDNA lipoplex and MCLs/pDNA lipoplex with MCLs with MAG 0.5 mg/mL concentration was similar, while with MCLs with MAG 1.0 mg/mL concentration, the luciferase activity was lower. This result was in agreement with a reported result that indicates transfection efficiency of MCLs (MAG 0.5 mg/mL) was similar to that of CLs in the absence of a magnetic field.31 The relatively lower gene transfection efficiency of MCLs/ pDNA lipoplex with higher MAG content (1.0 mg/mL) may be due to the extra cellular toxicity32 and to increasing the size of particle (Table 1).

Enhanced transfection efficiency of MCLs/pDNA complexes by magnetic induction

After optimizing the transfection condition, the effects of n class="Chemical">magnetic field exposure times (30 anpan>d 60 minpan>utes) followed by different inpan>cubationpan> times (4 anpan>d 6 hours) onpan> the tranpan>sfectionpan> efficiency were studied. pan> class="Chemical">Magnetic field exposure was applied at different times on transfected cells by MCLs (MAG 0.5 mg/mL). Longer magnetic field exposure times for transfected cells after 6 hours resulted in a decrease of luciferase activity, while after 4 hours luciferase activity (transfection efficiency) increased with longer exposure times (60 minutes) (Figure 7). The mechanism of MCLs/DNA lipoplex uptake into cells was possibly the as same as for CLs/DNA lipoplex, which was compatible with the comparable transfection activity of CLs/pDNA and MCLs/pDNA lipoplexes without magnetic induction. Magnetic nanoparticles are co-internalized with vectors into cells.33 Magnetic interaction between applied magnetic fields and magnetic nanoparticles could accelerate accumulation of the complexes on the surface of the cells. Then during the intracellular processing nanoparticles at a certain concentration may probably interact with the cell membrane, which could result in the nonspecific changes of membrane properties (such as ion transport potential and possibly fluidity) or destabilization of the endosomal environment.34 These could contribute to the rapid and effective gene delivery under a magnetic field. Therefore, it may be concluded that higher transfection efficiencies may arise from magnetic nanoparticle interaction with the cell membrane.

Figure 7

Effect of magnetic field exposure on transfection efficiency. (A) magnetic cationic liposomes (MCLs)/plasmid DNA (pDNA) lipoplex (MCLs with magnetite [MAG] 0.5 mg/mL concentration) after 6 hours’ incubation and (B) MCLs/pDNA lipoplex (MCLs with MAG 0.5 mg/mL concentration) after 4 hours’ incubation. These experiments were carried out under the optimal transfection conditions and magnetic induction time was varied from 30 to 60 minutes.

Abbreviation: RLU, relative light unit.

One of the most important aspects of transfection reagents is their n class="Disease">toxicity. The cytotoxic effect of different conpan>centrationpan>s of MCLs, CLs, PEI, and Lipofectamine on surviving CHO cells was investigated. Lower toxic effects on CHO cell viability were observed for CLs and MCLs (MAG 0.5 and 1.0 mg/ mL), while a major reduction in viable cells was observed for PEI treatment. The percentage of survival after exposure to CLs (90%), MCLs (MAG 0.5 mg/mL, 85%), and MCLs (MAG 1.0 mg/mL, 84%) were observed. In comparison with PEI (15%) and Lipofectamine (70%), a less cytotoxic effect with our lipoplex preparations was observed (Figure 8).

Figure 8

Viability of Chinese hamster ovary cells treated with cationic liposomes (CLs), magnetic cationic liposomes (MCLs), polyethyleneimine (PEI), and Lipofectamine™. Cells were seeded at 105 cells/mL in a 96-well plate and incubated at 37°C. Percentage of cell viability was determined following 24-hour exposure to varying amounts of MCLs (0.5 and 1 mg/mL of magnetite).

Note: Data represents the percentage of cell viability compared with untreated cells and cells treated with Lipofectamine and PEI as controls.

Conclusion

Based on the results presented here, the authors conclude that the liposome compositions (n class="Chemical">DPPC/DOAB anpan>d pan> class="Chemical">DPPC/ Chol/DOAB) prepared with high transfection efficiency may be useful in gene delivery in vitro. For the first time, using magnetic nanoparticles in these compositions brought about suitable transfection efficiency, although a reduction in cytotoxicity due to reduced incubation time was observed. An increase in concentrations of MAG incorporated into the liposomes and complexes in turn increases the particle size of MCLs and MCLs/pDNA complexes. MCLs/pDNA can respond to an external magnetic field and this quality is effective in conducting MCLs to the desired tissue in gene delivery and in reducing side effects in drug delivery systems. Moreover, transfection efficiency of a red-emitter mutant (S284T) pGL3 by different liposome composition was investigated. Results showed the mutant gene with red-emitting luciferase activity is transfected efficiently by different vehicle. Therefore, it may be suggested, this plasmid vector with red-emitter luciferase can be suitable for in vivo studies. In an overview the MCLs may be considered in gene delivery systems for conducting them to the target tissue in vivo.