Acceleration of gene transfection efficiency in neuroblastoma cells through polyethyleneimine/poly(methyl methacrylate) core-shell magnetic nanoparticles.

BACKGROUND: The purpose of this study was to demonstrate the potential of magnetic poly(methyl methacrylate) (PMMA) core/polyethyleneimine (PEI) shell (mag-PEI) nanoparticles, which possess high saturation magnetization for gene delivery. By using mag-PEI nanoparticles as a gene carrier, this study focused on evaluation of transfection efficiency under magnetic induction. The potential role of this newly synthesized nanosphere for therapeutic delivery of the tryptophan hydroxylase-2 (TPH-2) gene was also investigated in cultured neuronal LAN-5 cells.
METHODS: The mag-PEI nanoparticles were prepared by one-step emulsifier-free emulsion polymerization, generating highly loaded and monodispersed magnetic polymeric nanoparticles bearing an amine group. The physicochemical properties of the mag-PEI nanoparticles and DNA-bound mag-PEI nanoparticles were investigated using the gel retardation assay, atomic force microscopy, and zeta size measurements. The gene transfection efficiencies of mag-PEI nanoparticles were evaluated at different transfection times. Confocal laser scanning microscopy confirmed intracellular uptake of the magnetoplex. The optimal conditions for transfection of TPH-2 were selected for therapeutic gene transfection. We isolated the TPH-2 gene from the total RNA of the human medulla oblongata and cloned it into an expression vector. The plasmid containing TPH-2 was subsequently bound onto the surfaces of the mag-PEI nanoparticles via electrostatic interaction. Finally, the mag-PEI nanoparticle magnetoplex was delivered into LAN-5 cells. Reverse-transcriptase polymerase chain reaction was performed to evaluate TPH-2 expression in a quantitative manner.
RESULTS: The study demonstrated the role of newly synthesized high-magnetization mag-PEI nanoparticles for gene transfection in vitro. The expression signals of a model gene, luciferase, and a therapeutic gene, TPH-2, were enhanced under magnetic-assisted transfection. An in vitro study in neuronal cells confirmed that using mag-PEI nanoparticles as a DNA carrier for gene delivery provided high transfection efficiency with low cytotoxicity.
CONCLUSION: The mag-PEI nanoparticle is a promising alternative gene transfection reagent due to its ease of use, effectiveness, and low cellular toxicity. The mag-PEI nanoparticle is not only practical for gene transfection in cultured neuronal cells but may also be suitable for transfection in other cells as well.

Introduction

Magnetic nanoparticles have previously been used in biomedical applications, especially in the area of medical imaging,1 and drug and gene delivery.2 Magnetic-assisted gene transfection could improve n class="Disease">transfection efficiency by using magnetic force induction to introduce a therapeutic genpan>e into a target cell. Application of anpan> externpan>al magnetic field for genpan>e delivery was first reported by Mah et al.3 Magnetic microparticles were coated with adenpan>oassociated virus enpan>coding greenpan> fluorescenpan>t protein. It was demonstpan> class="Species">rated that adenoassociated virus conjugated with magnetic microparticles enhanced transduction efficiency both in vitro and in vivo. Since then, several intensive studies of magnetic-based gene delivery have been performed.4,5 Magnetic-assisted gene delivery can be applied to transfection reagents and gene therapeutic vehicles, and the first report focusing on the use of magnetic-assisted targeted gene delivery used polyethyleneimine (PEI)-coated nanoparticles for in vitro gene transfection.6 The study demonstrated the advantages of magnetic-assisted transfection in terms of reducing incubation time and DNA dose. To date, magnetic-assisted transfection has been demonstrated as one of the approaches for nucleic acid transfer, including DNA and RNA interference, in various cell lines. For example, the combination of cationic lipid-coated magnetic nanoparticles with transferrin and PEI was developed for transfection in a human cervical cancer cell line. This system enhanced the transfection efficiency by approximately 300-fold compared with control transfection reagents in the presence of an external magnetic field.5 A hybrid nanoparticle system consisting of superparamagnetic nanoparticles and PEI was used as a vehicle to transfer the interleukin-10 gene into vascular endothelial cells.4 This particle showed high transgene expression using a very low vector concentration and in a very short incubation time. This system is promising for treatment of patients with vascular disorders who require fast and target-specific delivery of the genes concerned. Apart from being an effective transfection reagent, incorporation of magnetic nanoparticles into lipid-based or polymeric-based carriers has also been considered as an alternative approach for improvement of non-viral vector-based gene therapy.7,8 At present, many research groups are aiming to develop a vehicle which could facilitate gene therapy in several genetic disorders, including the hematological,9 cardiovascular,10 and immunogenic systems.11

Non-viral approaches for nucleic acid delivery have also become a novel strategy for treating pan> class="Disease">neurological disease.12 Neuron-targeted nucleic acid therapy remains one of the few options available for the treatment of neurodegenerative disease. In previous studies, viral vectors were used as the gene carrier for transfer of nucleic acid into target neuron cells, and adenoassociated virus was the most common viral vector for gene transfection.13–15 However, there has been a recent focus on non-viral vector-based gene vectors for neuron systems, with some reported examples, including lipid-based and polymeric-based carriers. PEGylated immunoliposome-mediated brain-specific delivery of a gene encoding tyrosine hydroxylase for the treatment of patients with Parkinson’s disease has been studied successfully in an animal model.16 Modified transfection reagents, ie, PEI-PEG and Tet1 complexes, demonstrated increased luciferase expression levels in neural progenitor cells compared with unmodified PEI-PEG complexes.17

In this study, we investigated the use of novel synthesized magnetic nanoparticles for gene delivery in neuronal cells. Magnetic n class="Chemical">PEI/pan> class="Chemical">poly(methyl methacrylate) (PMMA) core-shell (mag-PEI) nanoparticles were prepared using ultrasonication-assisted emulsifier-free emulsion polymerization. Loading of magnetic nanoparticles enhanced gene transfection efficiency by accelerating the cellular uptake of nanoparticles. The physicochemical properties and morphology of the mag-PEI nanoparticles were characterized, and a feasibility study was performed to evaluate the gene transfection efficiency of the mag-PEI nanoparticles using plasmid pGL3-basic containing cytomegalovirus (CMV) promoter/enhancer encoding the luciferase reporter gene (pGL3-CMV). In vitro transfection of pGL3-CMV could be measured quantitatively using the luciferase assay system. Different N/P ratios of magnetoplex were prepared to investigate the transfection efficiency at different transfection times with and without magnetic induction. The cytotoxicity of the mag-PEI nanoparticles was examined using the MTT assay. Transfection under magnetic induction strongly promotes cell internalization, as shown by confocal laser scanning microscopy. Optimal conditions were selected for transfection of pGL3-CMV, a plasmid containing tryptophan hydroxylase-2 (TPH-2), a rate-limiting enzyme for production of the serotonin neurotransmitter.18 This study proposes an alternative nanocarrier, which is applicable for neuronal gene therapy.

Materials and methods

Materials

n class="Chemical">Ferrous chloride tetrahydrate (FeCl2·4H2O), pan> class="Chemical">ferric chloride hexahydrate (FeCl3·6H2O), methyl methacrylate (MMA), and t-butyl hydroperoxide were purchased from Fluka (St Louis, MO). PEI (molecular weight of 25 kDa) was purchased from Sigma-Aldrich (St Louis, MO). All chemicals were of analytical grade and used for synthesis of magnetic core/shell nanoparticles. Lipofectamine 2000™ was purchased from Invitrogen (Carlsbad, CA). A PolyMAG and magnetoFACTOR-96 plate was purchased from Chemicell GmbH (Berlin, Germany). Plasmid pGL3-basic containing CMV promoter/enhancer, which is an expression vector for human cell lines, was used to monitor transfection efficiency.19 Plasmid DNA was propagated in Escherichia coli, which were grown in Lysogeny broth (10 g/L tryptone, 5 g/L yeast extract, and 10 g/L NaCl), and supplemented with ampicillin under shaking conditions of 250 rounds per minute at 37°C. The plasmid was extracted using the PureLink™ Hipure Plasmid DNA purification kit (Invitrogen). The extracted plasmid was observed by electrophoresis on 1.0% agarose gel. Plasmid purity and concentration were determined by measuring light absorbance at 260 nm and 280 nm using a SpectraMax M2 microplate reader (MDS Inc, Sunnyvale, CA). Primers for reverse-transcriptase polymerase chain reaction (RT-PCR) of GAPDH and TPH-2 genes are listed in Table 1.

Table 1

Polymerase chain reaction primers used for tryptophan hydroxylase-2 cloning and semiquantitative assay of GAPDH and tryptophan hydroxylase-2 gene expressions

Primer	Sequence	Product (Kb)	Reference
TPH-2-NheI_pGL-CMV	5′-CCT gCT AgC gCC TTC CTC TCA ATC TC-3′	1.5	The present study
TPH-2-XbaI_pGL-CMV	5′-CCC gCT CTA gAT AgT TCC Agg CAT CAA ATC C-3′
GAPDH sense	5′-gAC CAC AgT CCA TgC CAT CAC T-3′	0.4	Divya and Pillai22
GAPDH antisense	5′-TCC ACC ACC CTg TTg CTg TAg-3′
TPH-2 sense	5′-AAC CAC TAT TgT gAC gCT gAA TCC TCC AgA gAA-3′	0.2	The present study
TPH-2 antisense	5′-ACC CAT AAC CCA TCg CCA CAT CCA CAA AA-3′

Abbreviations: CMV, cytomegalovirus; TPH-2, tryptophan hydroxylase-2.

Preparation of magnetic core/shell nanoparticles

n class="Chemical">Mag-PEI nanpan>oparticles with a pan> class="Chemical">PMMA core and PEI shell were prepared by emulsion polymerization.20 In brief, iron oxide at a concentration of 25 mg was dispersed thoroughly with 2 g of MMA using an ultrasonicator for 5 minutes. For a total of 50 g of solution, the iron oxide-MMA dispersion was mixed with 47 g of PEI solution containing 0.5 g of PEI using a homogenizer (Sonics Vibra cell, amplitude 40%). The dispersion was homogenized for 15 minutes and then transferred into a water-jacketed flask equipped with a condenser, a magnetic stirrer, and a nitrogen inlet. The dispersion was purged with nitrogen for 30 minutes, followed by addition of t-butyl hydroperoxide aqueous solution (1 g, 0.5 mM) to initiate polymerization. The mixture was then continuously stirred at 80°C for 2 hours in a nitrogen environment. After the reaction, the particle dispersion was purified by repeated centrifugation (13,000 rpm), decantation, and redispersion until the conductivity of the supernatant was close to that of the distilled water used. The amine density on the surface of the nanoparticles was evaluated using a typical acid-base titration method.21 The titration was carried out with an Autotitrator (Mettler Toledo, T50, Columbus, OH) and a pH glass sensor (Mettler Toledo, DGi115-SC) using 0.01 M NaOH standardized by potassium hydrogen phthalate as a titrant. The sample preparation was performed using an aqueous solution composed of 0.5 mL of the sample suspension (30–40 mg/mL), 50 mL of deionized water, and 0.40 mL of 0.1 M HCl. Each value reported was an average of at least three measurements. The characteristics of mag-PEI nanoparticles were then observed through a transmission electron microscope at an accelerating voltage of 80 kV.

Preparation of magnetoplex

For the feasibility study of n class="Chemical">mag-PEI nanpan>oparticles in genpan>e delivery, plasmid DN anpan>d pan> class="Gene">pGL3-CMV encoding the luciferase reporter gene at a concentration of 1 mg/mL was mixed with mag-PEI nanoparticles at the same concentration to form the mag-PEI nanoparticle/DNA magnetoplex. The magnetoplex was prepared at various N/P ratios, ie, 0.4/1, 0.8/1, 1.6/1, 4.3/1, 8.7/1, and 17.5/1. The solutions of magnetoplex were subsequently incubated at room temperature for 30 minutes before use. The optimal N/P ratio from pGL3-CMV transfection was used for pGL3-CMV-TPH-2 transfection, in which the magnetoplex was prepared in the same manner.

Gel retardation assay

After forming the magnetoplex, loading dye was added and mixed before loading into 1.0% n class="Chemical">agarose gel. Electrophoresis was carried out at 100 V for 60 minutes. pan> class="Chemical">Agarose gel was stained in 1 μg/mL ethidium bromide. The presence of plasmid DNA was visible under an ultraviolet transilluminator (Syngene, Cambridge, UK). The shifted bands, corresponding to free plasmid, were determined.

Atomic force microscopy analysis

Atomic force microscopic images of magnetoplex were obtained using a dynamic force microscope (Seiko SPA4000, Tokyo, Japan). All samples were prepared by dropping the magnetoplex solution onto a n class="Chemical">mica surface for air-dryinpan>g. All images were obtainpan>ed with a scanpan>ninpan>g speed of 1.0 Hz over a 2 μm × 2 μm area.

Size and zeta potential analysis

The mean zeta hydrodynamic diameter, polydispersity index, and surface charge of the magnetoplex were determined by dynamic light scattering using a Zetasizer Nano ZS (Malvern Instruments Ltd, Malvern, Worcestershire, UK) at room tempen class="Species">rature. The magnpan>etoplex was prepared anpan>d combined to achieve 1 mL in deionized pan> class="Chemical">water. All samples were measured in triplicate.

Cell culture

In this study, n class="Species">human pan> class="Disease">neuroblastoma (LAN-5) cells were used as the neuronal cell culture model. The cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco-BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (HyClone, South Logan, UT) and incubated for 24 hours at 37°C with 5% CO2 before use.

Transfection and cytotoxicity

To evaluate the n class="Disease">transfection efficiency of the pan> class="Chemical">mag-PEI nanoparticles, LAN-5 cells were seeded into a 96-well plate at a density of 5 × 104 cells per well. Before transfection, the medium was removed, the cells were rinsed with phosphate-buffered saline twice, and then plated and incubated with serum-free Dulbecco’s modified Eagle’s medium. Cells were incubated with the magnetoplex at 37°C for 15, 30, 60, 120, and 180 minutes with or without magnetoFACTOR-96, in serum-free medium which was then replaced with growth medium. Twenty-four hours after transfection,19 luciferase activity was determined in accordance with the manufacturer’s recommendations (Promega, Madison, WI). Luciferase activity was quantified as relative light units using a luciferase assay system (Promega). Luciferase activity was normalized for protein concentration using the Bradford assay. The commercial transfection reagents, Lipofectamine 2000 and PolyMAG, were used as positive controls for comparison of their transfection efficiency with our synthesized mag-PEI nanoparticles. Naked DNA (DNA transfected without a gene carrier) was used as the negative control for transfections. The Lipofectamine/DNA complex and PolyMAG/DNA magnetoplex were prepared according to the manufacturer’s directions.

n class="Chemical">MTT assays were performed to evaluate cell viability after treatmenpan>t with magnetoplex. LAN-5 cells were seeded at the same denpan>sity used for tranpan>sfection. The cells were cultured at 37°C unpan>der 5% pan> class="Chemical">CO2 overnight. The assay was performed 24 hours after transfection according to the manufacturer’s recommendation. Percentage viability was calculated for cells transfected with naked DNA.

Magnetoplex internalization into cells

LAN-5 cells were seeded onto glass coverslips in 6-well plates at densities of 7.5 × 105 cells per well. Before transfection, the medium was removed, the cells were rinsed with n class="Chemical">phosphate-buffered saline twice, anpan>d thenpan> plated anpan>d incubated with serum-free Dulbecco’s modified Eagle’s medium. Cells were incubated with pan> class="Chemical">rhodamine-B-isothiocyanate (RITC)-labeled mag-PEI nanoparticle/DNA magnetoplexes at 37°C for 60 and 180 minutes with and without a magnetoFACTOR-96 plate in serum-free medium which was then replaced with growth medium. Twenty-four hours after transfection, the transfected cells were stained with acridine orange then washed with phosphate-buffered saline twice and visualized under a confocal laser scanning microscope (LSM 700, Carl Zeiss Inc, Oberkochen, Germany) with a 100× objective lens under 405 nm excitation for acridine orange and 561 nm excitation for RITC. The results were analyzed using LSM 700 ZEN software.

Isolation of TPH-2, cloning, and construction of expression vector

cDNA for the n class="Gene">TPH-2 genpan>e was synpan>thesized by RT-PCR using pan> class="Species">human brain medulla oblongata total RNA (Clontech cDNA panels, BD Biosciences, Franklin Lakes, NJ) as a template. The RT-PCR reaction was performed using ImPromt-II™ reverse transcriptase in accordance with the manufacturer’s recommendations (Promega). The resulting cDNAs were used as a template for PCR using TPH-2-NheI_pGL-CMV and TPH-2-XbaI_pGL-CMV as forward and reverse primers, respectively (Table 1). The specific PCR products were then cloned into the pGEM-T™ easy vector (Promega) to verify TPH-2 sequences by restriction enzyme digestion using HindIII (Fermentas, Glen Burnie, MD) and XbaI (NEB, Hitchin, UK) as the restriction enzyme and confirmed this result by DNA sequencing. The TPH-2 gene was finally cloned into pGL3-basic containing CMV promoter/enhancer, generating pGL3-CMV-TPH-2.

Monitoring of TPH-2 expression by RT-PCR

To determine n class="Gene">TPH-2 expression, LAN-5 cells were seeded into 6-well plates at a denpan>sity of 7.5 × 105 cells per well. pan> class="Gene">pGL3-CMV-TPH-2 was mixed with mag-PEI nanoparticles to prepare magnetoplex at an N/P ratio of 0.8, which was previously optimized. PolyMAG and Lipofectamine 2000 were used as positive controls and naked DNA was used as a negative control for transfection. Cells were incubated with the magnetoplex at 37°C for 60 minutes with and without external magnetic induction in serum-free medium which was then replaced with growth medium. Twenty-four hours after transfection, RNA extraction with TRIzol (Invitrogen) was performed according to the manufacturer’s recommendations. The quantity and integrity of the RNA obtained were evaluated by spectrophotometry and gel electrophoresis stained with ethidium bromide. The RNA samples obtained were then treated with a deoxyribonuclease I amplification grade kit (P romega) at 37°C for 30 minutes to eliminate any contaminated DNA. Two steps of RT-PCR were carried out using Impromt II reverse transcription to synthesize first-strand cDNA. Taq polymerase (NEB) was then used for PCR under the following conditions: 95°C over 2 minutes for the TPH-2 gene and 94°C over 5 minutes for the GAPDH gene, followed by 35 cycles of denaturation (95°C over 30 seconds for the TPH-2 gene and 94°C over 15 seconds for the GAPDH gene), annealing (60°C over 30 seconds for the TPH-2 gene and 55°C over 15 seconds for the GAPDH gene), extension (68°C over 30 seconds for the TPH-2 gene and 72°C over 15 seconds for the GAPDH gene), and finally a single extension (68°C over 10 minutes for the TPH-2 gene and 72°C for 15 minutes for the GAPDH gene). A control negative RT-PCR was performed in the absence of reverse transcriptase to check for DNA contamination in the RNA preparation. Each TPH-2 expression was normalized against expression of the GADPH gene to eliminate the effect of the cell population. Each relative TPH-2 expression was then compared with naked DNA transfected cells.

Statistical analysis

Experiments were carried out in triplicate. The independent Student’s t-test was used for the statistical analysis, with P < 0.05 considered to be statistically significant.

Results and discussion

Fabrication of core shell nanoparticles

Transmission electron microscopy revealed that we could obtain magnetic polymeric core/shell nanospheres, ie, n class="Chemical">mag-PEI nanpan>oparticles, with high magnetic nanpan>oparticle loading (Figure 1). The size distribution was founpan>d to be narrow, as indicated in the histogram. The zeta potenpan>tial determined by dynpan>amic light scattering indicated that the pan> class="Chemical">mag-PEI nanoparticles had positive surface charges around 39.3 ± 1.9 mV.

Figure 1

Transmission electron microscopic image. (A) Mag-PEI and histogram showing size distribution. (B) Mag-PEI nanoparticles with high magnetic loading.

Abbreviation: Mag-PEI, magnetic poly(methyl methacrylate) core/ polyethyleneimine shell.

DNA binding affinity and magnetoplex formation were confirmed using the gel n class="Disease">retardation assay. One microgram of plasmid pan> class="Gene">pGL3-basic containing the CMV promoter/enhancer was applied to a prepared magnetoplex with mag-PEI nanoparticles at different N/P ratios. Trailing of DNA disappeared in the gel at an N/P ratio of 0.8/1 (Figure 2). The results showed that plasmid DNA was adsorbed onto the mag-PEI nanoparticle surface by electrostatic interaction, resulting in the magnetoplex. Our cationic mag-PEI nanoparticles could neutralize the negative charge of plasmid DNA and increase the mag-PEI nanoparticle-induced cationic properties of the magnetoplex, corresponding to the results of the dynamic light scattering analysis (Table 2).

Figure 2

Gel retardation assay.

Notes: One microgram of plasmid DNA was applied to the magnetoplex with mag- PEI nanoparticles at different N/P ratios. Lane 1 is the control DNA without mag-PEI nanoparticles. Lanes 2–7 represent mag-PEI NP/DNA magnetoplexes with N/P ratios of 0.4/1, 0.8/1, 1.6/1, 4.3/1, 8.7/1, and 17.5/1.

Abbreviation: Mag-PEI, magnetic poly(methyl methacrylate) core/polyethyleneimine shell.

Table 2

Size and zeta potential of mag-PEI nanoparticle/DNA magnetoplex at N/P ratios of 0.4/1, 0.8/1, 1.6/1, 4.3/1, 8.7/1, and 17.5/1

	N/P	Size (nm)	Zeta potential (mV)	PDI
Mag-PEI NP		123.8 ± 3.1	39.3 ± 1.9	0.26 ± 0.02
Mag-PEI NP/DNA	0.4/1	231.3 ± 24.7	6.3 ± 1.7	0.36 ± 0.03
	0.8/1	370.0 ± 32.4	25 ± 1.4	0.45 ± 0.05
	1.6/1	298.5 ± 65	34.8 ± 2.3	0.75 ± 0.15
	4.3/1	286.8 ± 23.3	22.4 ± 3.2	0.42 ± 0.06
	8.7/1	251.3 ± 5.1	5.6 ± 0.5	0.34 ± 0.06
	17.5/1	215.4 ± 17.5	3.3 ± 0.7	0.36 ± 0.05

Abbreviations: Mag-PEI NP, magnetic poly(methyl methacrylate) core/polyethyleneimine shell nanoparticles; PDI, polydispersity index.

Magnetoplex formation

The morphology and size of the magnetoplex were analyzed under atomic force microscopy at two different N/P ratios, ie, 0.8 anpan>d 4.3. Atomic force microscopy detected that the magnetoplex appearanpan>ce was spherical, corresponding to the core structures, ie, pan> class="Chemical">mag-PEI nanoparticles (Figure 3). It is likely that addition of more mag-PEI nanoparticles with N/P ratios in the range of 0.8/1–4.3/1 could improve the magnetoplex condensation. This result correlated well with size analyzed by dynamic light scattering (Table 2). However, magnetoplex distribution changed in response to changes in the N/P ratio, as shown at ratios of 0.8/1 and 4.3/1 (Figure 3). As a result, use of excess mag-PEI nanoparticles caused aggregation of the magnetoplex (Figure 3C), which may have interrupted cell transfection. Therefore, the magnetoplex formed at an N/P ratio of 0.8/1 was selected for cell transfection in further studies.

Figure 3

Atomic force microscopy images of mag-PEI nanoparticles (A) mag-PEI nanoparticles forming magnetoplexes with DNA at N/P ratios of 0.81/1 (B) and 4.3/1 (C).

Abbreviation: Mag-PEI, magnetic poly(methyl methacrylate) core/polyethyleneimine shell.

The size and zeta potential of the magnetic nanoparticles were determined at pH 7.4. During magnetoplex formation, a dynamic change in size and charge occurred at N/P ratios in the ranpan>ge of 0.4–17.5 (Table 2). The size of the pan> class="Chemical">mag-PEI/ DNA was larger than that of mag-PEI, indicating that adsorption of DNA had occurred on the particle surface. With a constant amount of DNA, the total charges at each N/P ratio were dependent on the amount of mag-PEI nanoparticles added to the DNA solution. At N/P ratios in the 0.4–1.6 range, the charges increased according to the amount of mag-PEI nanoparticles added. However, at N/P ratios in the range of 4.3–17.5, the excess amount of mag-PEI nanoparticles destabilized the complex, as indicated by a decrease in zeta potential.

Optimal transfection conditions and transfection efficiency

Gene transfection was investigated in the n class="Species">human LAN-5 pan> class="Disease">neuroblastoma cell line. Cells were transfected with the magnetoplex at an optimal N/P ratio of 0.8. Gene transfection was performed by incubation of the magnetoplex with cells for 15, 30, 60, 120, and 180 minutes in the presence and absence of an external magnetic plate. Transfection via Lipofectamine 2000 and PolyMAG, two commercial transfection reagents, was carried out in the positive controls. Luciferase signals expressed in transfected cells were determined quantitatively. At all tested N/P ratios, the results confirm that magnetic-induced transfection was a very effective system for gene transfection (Figure 4A). Luciferase expression levels were enhanced when DNA transfections were stimulated under magnetic force using the magnetoFACTOR-96 plate. Our results show that incorporation of magnetic nanoparticles in polymeric-based vectors is an effective strategy to elevate the transfection signal and shorten the transfection time. The efficiency of gene transfection was increased through physical stimulation by an external magnetic field. Among the N/P ratios in the range of 0.4–17.5, the highest transfection efficiency was obtained at an N/P ratio of 0.8. This result indicates that transfection efficiency was affected by several physicochemical properties of the magnetoplex. With a low amount of mag-PEI nanoparticles (N/P ratio < 0.8), the DNA strands are not completely adsorbed onto the nanoparticles. Therefore, the DNA delivered into the cells is not properly protected and easily digested by intracellular enzymes. The N/P ratio of 0.8 is probably the optimal condition, including for size, zeta potential, and complex stability. Although at an N/P ratio of 1.6–4.3 the magnetoplex also has an appropriate size and zeta potential, it can also cause cell membrane damage due to the greater number of nanoparticles with a positive surface charge added to the system. Furthermore, the atomic force microscopy results indicated that the magnetoplex at an N/P ratio of 4.3 was agglomerated, which was an unsuitable condition for transfection. Therefore, to obtain high transfection efficiency, several factors needed to be compromised.

Figure 4

Transfection efficiency (A) and cytotoxicity (B) of mag-PEI nanoparticles at 15, 30, 60, 120, and 180 minutes in LAN-5 cells.

Notes: The transfection efficiency and cytotoxicity was compared with positive control Lipofectamine 2000™, PolyMAG, and negative controls (naked DNA, plasmid pGL-3-basic containing CMV promoter/enhancer). *Significant differences between cells transfected with and without a magnetic plate in each transfection reagent (P < 0.05). The gray and white bars show the results of cells incubated with or without magnetic induction, respectively.

Abbreviation: Mag-PEI, magnetic poly(methyl methacrylate) core/polyethyleneimine shell.

Unlike for n class="Chemical">PolyMAG, the results indicate that the increased pan> class="Disease">transfection efficiency for mag-PEI nanoparticles is time-dependent. PolyMAG is a commercially available carrier enhancing the transfection signal within a short induction time, and expression levels are fairly constant at different incubation times. The difference in improvement of transfection over time is probably due to the difference in magnetic properties between PolyMAG and mag-PEI nanoparticles. PolyMAG has very strong magnetic properties, which strongly enforces cell internalization of particles into the cell within a short time. However, after 120 minutes of induction, the transfection efficiency obtained from mag-PEI nanoparticles was about the same level as that obtained from PolyMAG, and was increased after 180 minutes of induction time. Apparently, for LAN-5 cells, a magnetic-assisted transfection system is more effective than a liposome-based system like Lipofectamine 2000, and there was no statistically significant difference between cells transfected with and without a magnetic plate.

Evaluation of cytotoxicity

In this study, the n class="Disease">toxicity of pan> class="Chemical">mag-PEI nanoparticles towards LAN-5 cells was investigated using the MTT assay. Cells were treated with the magnetoplex under the same conditions as the transfection procedures. The viability of LAN-5 cells after transfection was in the range of 80%–100% when incubated with magnetoplex at N/P ratios of 0.4/1, 0.8/1, 1.6/1, 4.3/1, 8.7/1, and 17.5/1 for 15, 30, 60, 120, and 180 minutes (Figure 4B). Viability of cells exposed to magnetic induction was lower than that of unexposed cells. However, the differences were not statistically significant. Therefore, this result verifies that the cytotoxicity of mag-PEI nanoparticles is very low, making these particles suitable for use in gene therapy.

Cellular internalization

Visualization of uptake of n class="Chemical">mag-PEI nanpan>oparticles into LAN-5 cells was observed by confocal laser scanpan>ning microscopy. The pan> class="Chemical">RITC-labeled mag-PEI nanoparticle/DNA magnetoplex at an N/P ratio of 0.8/1 was incubated with the cells for 60 and 180 minutes. The incubations were done separately with and without external magnetic induction. At 24 hours after transfection, confocal laser scanning microscopy images revealed the degree of intensity of the magnetoplex entering into LAN-5 cells (Figure 5). At both 60 and 180 minutes of incubation, the intensities were significantly increased when transfection was performed under magnetic induction. The results indicate that the magnetoplex distributed into the intracellular compartment, the cytoplasm, and the region of the nucleus. Internalization was confirmed by confocal Z-stack image scanning (data not shown). The result corresponded well with the luciferase activity in Figure 4A. This provides more evidence of acceleration of the transfection period through magnetoplex transfection in neuronal cells.

Figure 5

Confocal image of LAN-5 cells 24 hours after transfection. Cells incubated with or without a magnetic plate for (A) 60 minutes and (B) 180 minutes were used for investigation of the cellular uptake of mag-PEI nanoparticles.

Note: Green, acridine orange-stained live cells; red, RITC-stained mag-PEI nanoparticles.

Abbreviations: RITC, rhodamine-B-isothiocyanate; Mag-PEI, magnetic poly(methyl methacrylate) core/polyethyleneimine shell.

TPH-2 cloning

cDNA synthesized from n class="Species">human brain medulla pan> class="Disease">oblongata total RNA was used as a template for synthesizing the TPH-2 gene fragment. PCR was performed using the specific primers described in Table 1. The PCR product showed a specific band at 1.5 kilobases under an ultraviolet transilluminator (Syngene, Cambridge, UK). The band was cut and ligated into a pGEM®-T vector (Promega). DNA sequencing verified that the isolated PCR product had 99.5% similarity to Homo sapiens TPH-2 mRNA. The TPH-2 gene was then finally transferred into pGL3-CMV basic containing the CMV promoter/enhancer.19 The resulting plasmid was used for gene transfection into LAN-5 cells.

Role of mag-PEI nanoparticles as a carrier for TPH-2 expression

The aforementioned data indicate that n class="Chemical">mag-PEI nanpan>oparticles are a promising carrier for magnetic-assisted tranpan>sfection due to their effectivenpan>ess, with low pan> class="Disease">cytotoxicity and a short transfection time. We are continuing to test mag-PEI nanoparticles at an N/P ratio of 0.8/1 as a carrier for delivery of the neuronal TPH-2 therapeutic gene into LAN-5 cells. The magnetic induction time was fixed at 60 minutes. After transfection, the cells were incubated for 24 hours and total RNA was isolated by the TRIzol reagent, as described earlier. Expression of the TPH-2 gene was measured by RT-PCR using isolated total RNA as a template. PCR products from the housekeeping gene, GAPDH, were used to normalize the gene expression values. As a result, mag-PEI nanoparticles showed efficiency in induction of TPH-2 expression comparable with that of PolyMAG (Figure 6). Cells transfected with the mag-PEI nanoparticle/pGL3-CMV-TPH-2 magnetoplex under magnetic induction showed a signal that was 13 times stronger than that obtained without induction. We compared the effectiveness of mag-PEI nanoparticles for therapeutic gene delivery with that of a liposome-based system, ie, Lipofectamine 2000. The results show that the difference between TPH-2 expression in cells transfected with and without magnetic induction was not significantly different. Therefore, this study demonstrates the potential of our synthesized nanoparticle for magnet-assisted gene transfection. Mag-PEI nanoparticles successfully enhanced the transfection efficiency of TPH-2 gene delivery.

Figure 6

Semiquantitative reverse-transcriptase polymerase chain reaction result shows expression of the TPH-2 gene in LAN-5 24 hours after transfection by mag-PEI nanoparticles compared with positive control Lipofectamine 2000™ and PolyMAG, and negative control (naked DNA).

Notes: *Significant differences between cells transfected with and without magnetic plate in each transfection reagent (P < 0.05). The gray bars and white bars show the results of cells incubated with and without magnetic induction, respectively.

Abbreviations: Mag-PEI, magnetic poly(methyl methacrylate) core/polyethyleneimine shell; TPH-2, tryptophan hydroxylase-2.

Conclusion

In this study, we demonstn class="Species">rated the potenpan>tial of pan> class="Chemical">mag-PEI nanoparticles, possessing high saturation magnetization, for gene transfection in vitro. The mag-PEI nanoparticles at an N/P ratio of 0.8/1 showed the highest transfection efficiency and low cytotoxicity in neuronal LAN-5 cells. The results obtained from the luciferase assay were consistent with those of the cell internalization investigation by confocal laser scanning microscopy. Significant acceleration of transfection efficiency within a short induction time revealed that mag-PEI nanoparticles are a promising alternative carrier for gene delivery. This newly improved magnetic nanoparticle is suitable for magnetic-assisted transfection, which may be further applied in gene therapy for neuropsychiatric and other diseases.