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Literature DB >> 8308299

An improved MTT assay using the electron-coupling agent menadione.

H Garn¹, H Krause, V Enzmann, K Drössler.

Abstract

A modification of the MTT based tetrazolium colorimetric assay is described. Using the electron-coupling agent menadione formazan formation by murine splenocytes and P-815 cells was significantly increased whereas dye reduction by macrophages was hardly influenced. These observations suggest that it should be possible to improve the tetrazolium based cytotoxicity assays of murine macrophages against cells of the syngeneic tumour cell line P-815.

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Year: 1994 PMID： 8308299 DOI： 10.1016/0022-1759(94)90062-0

Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303

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Cited

17 in total

1. Evaluation of a colorimetric antifungal susceptibility test by using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride.

Authors: Jeong Hwan Shin; Jae-Cheol Choi; Jeong Nyeo Lee; Hyung Hoi Kim; Eun Yup Lee; Chulhun Ludgerus Chang
Journal: Antimicrob Agents Chemother Date: 2004-11 Impact factor: 5.191

2. De novo generation of cationic antimicrobial peptides: influence of length and tryptophan substitution on antimicrobial activity.

Authors: Berthony Deslouches; Shruti M Phadke; Vanja Lazarevic; Michael Cascio; Kazi Islam; Ronald C Montelaro; Timothy A Mietzner
Journal: Antimicrob Agents Chemother Date: 2005-01 Impact factor: 5.191

3. Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of rifampin-resistant Mycobacterium tuberculosis.

Authors: R N Mshana; G Tadesse; G Abate; H Miörner
Journal: J Clin Microbiol Date: 1998-05 Impact factor: 5.948

4. Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells.

Authors: Su-Wen Lim; Hwei-San Loh; Kang-Nee Ting; Tracey Dawn Bradshaw; Zeenathul Nazariah Allaudin
Journal: Trop Life Sci Res Date: 2015-04

5. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.

Authors: B Jahn; E Martin; A Stueben; S Bhakdi
Journal: J Clin Microbiol Date: 1995-03 Impact factor: 5.948

10. Evaluation of the broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride for rapidly growing mycobacteria susceptibility testing.

Authors: Sun Min Lee; Jeong man Kim; Joseph Jeong; Young Kil Park; Gill-Han Bai; Eun Yup Lee; Min Ki Lee; Chulhun L Chang
Journal: J Korean Med Sci Date: 2007-10 Impact factor: 2.153

An improved MTT assay using the electron-coupling agent menadione.

1. Evaluation of a colorimetric antifungal susceptibility test by using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride.

2. De novo generation of cationic antimicrobial peptides: influence of length and tryptophan substitution on antimicrobial activity.

3. Use of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide for rapid detection of rifampin-resistant Mycobacterium tuberculosis.

4. Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells.

5. Susceptibility testing of Candida albicans and Aspergillus species by a simple microtiter menadione-augmented 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay.

6. RALBP1/RLIP76 depletion in mice suppresses tumor growth by inhibiting tumor neovascularization.

7. Genetic resistance of Egyptian chickens to infectious bursal disease and Newcastle disease.

8. Endothelial Cell Response to Fusobacterium nucleatum.

9. Oncolytic virus-mediated tumor radiosensitization in mice through DNA-PKcs-specific shRNA.

10. Evaluation of the broth microdilution method using 2,3-diphenyl-5-thienyl-(2)-tetrazolium chloride for rapidly growing mycobacteria susceptibility testing.