Literature DB >> 22002026

Super-resolution 3D microscopy of live whole cells using structured illumination.

Lin Shao1, Peter Kner, E Hesper Rego, Mats G L Gustafsson.   

Abstract

Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.

Mesh:

Year:  2011        PMID: 22002026     DOI: 10.1038/nmeth.1734

Source DB:  PubMed          Journal:  Nat Methods        ISSN: 1548-7091            Impact factor:   28.547


  18 in total

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Journal:  Nat Methods       Date:  2010-08-08       Impact factor: 28.547

2.  Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.

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Journal:  J Biophotonics       Date:  2010-07       Impact factor: 3.207

3.  Video-rate far-field optical nanoscopy dissects synaptic vesicle movement.

Authors:  Volker Westphal; Silvio O Rizzoli; Marcel A Lauterbach; Dirk Kamin; Reinhard Jahn; Stefan W Hell
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4.  Interferometric fluorescent super-resolution microscopy resolves 3D cellular ultrastructure.

Authors:  Gleb Shtengel; James A Galbraith; Catherine G Galbraith; Jennifer Lippincott-Schwartz; Jennifer M Gillette; Suliana Manley; Rachid Sougrat; Clare M Waterman; Pakorn Kanchanawong; Michael W Davidson; Richard D Fetter; Harald F Hess
Journal:  Proc Natl Acad Sci U S A       Date:  2009-02-06       Impact factor: 11.205

5.  Live-cell photoactivated localization microscopy of nanoscale adhesion dynamics.

Authors:  Hari Shroff; Catherine G Galbraith; James A Galbraith; Eric Betzig
Journal:  Nat Methods       Date:  2008-04-13       Impact factor: 28.547

6.  Three-dimensional sub-100 nm resolution fluorescence microscopy of thick samples.

Authors:  Manuel F Juette; Travis J Gould; Mark D Lessard; Michael J Mlodzianoski; Bhupendra S Nagpure; Brian T Bennett; Samuel T Hess; Joerg Bewersdorf
Journal:  Nat Methods       Date:  2008-05-11       Impact factor: 28.547

7.  Confined activation and subdiffractive localization enables whole-cell PALM with genetically expressed probes.

Authors:  Andrew G York; Alireza Ghitani; Alipasha Vaziri; Michael W Davidson; Hari Shroff
Journal:  Nat Methods       Date:  2011-02-13       Impact factor: 28.547

8.  Fast, three-dimensional super-resolution imaging of live cells.

Authors:  Sara A Jones; Sang-Hee Shim; Jiang He; Xiaowei Zhuang
Journal:  Nat Methods       Date:  2011-05-08       Impact factor: 28.547

9.  Kinetochore-driven formation of kinetochore fibers contributes to spindle assembly during animal mitosis.

Authors:  Helder Maiato; Conly L Rieder; Alexey Khodjakov
Journal:  J Cell Biol       Date:  2004-11-29       Impact factor: 10.539

10.  Whole-cell 3D STORM reveals interactions between cellular structures with nanometer-scale resolution.

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Journal:  Nat Methods       Date:  2008-11-23       Impact factor: 28.547

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  130 in total

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Authors:  David W Ehrhardt; Wolf B Frommer
Journal:  Plant Cell       Date:  2012-02-24       Impact factor: 11.277

2.  Time-lapse two-color 3D imaging of live cells with doubled resolution using structured illumination.

Authors:  Reto Fiolka; Lin Shao; E Hesper Rego; Michael W Davidson; Mats G L Gustafsson
Journal:  Proc Natl Acad Sci U S A       Date:  2012-03-19       Impact factor: 11.205

3.  Widely accessible method for superresolution fluorescence imaging of living systems.

Authors:  Peter Dedecker; Gary C H Mo; Thomas Dertinger; Jin Zhang
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-18       Impact factor: 11.205

Review 4.  The bacterial divisome: ready for its close-up.

Authors:  Veronica W Rowlett; William Margolin
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  2015-10-05       Impact factor: 6.237

5.  Dynamics and organization of cortical microtubules as revealed by superresolution structured illumination microscopy.

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Journal:  Plant Physiol       Date:  2014-03-31       Impact factor: 8.340

6.  ADVANCED IMAGING. Extended-resolution structured illumination imaging of endocytic and cytoskeletal dynamics.

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Journal:  Science       Date:  2015-08-28       Impact factor: 47.728

Review 7.  Maurer's clefts, the enigma of Plasmodium falciparum.

Authors:  Esther Mundwiler-Pachlatko; Hans-Peter Beck
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-27       Impact factor: 11.205

8.  Artifact-free whole-slide imaging with structured illumination microscopy and Bayesian image reconstruction.

Authors:  Karl A Johnson; Guy M Hagen
Journal:  Gigascience       Date:  2020-04-01       Impact factor: 6.524

Review 9.  Faster fluorescence microscopy: advances in high speed biological imaging.

Authors:  Peter W Winter; Hari Shroff
Journal:  Curr Opin Chem Biol       Date:  2014-05-09       Impact factor: 8.822

10.  Two-photon instant structured illumination microscopy improves the depth penetration of super-resolution imaging in thick scattering samples.

Authors:  Peter W Winter; Andrew G York; Damian Dalle Nogare; Maria Ingaramo; Ryan Christensen; Ajay Chitnis; George H Patterson; Hari Shroff
Journal:  Optica       Date:  2014-09-20       Impact factor: 11.104

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