| Literature DB >> 22002026 |
Lin Shao1, Peter Kner, E Hesper Rego, Mats G L Gustafsson.
Abstract
Three-dimensional (3D) structured-illumination microscopy (SIM) can double the lateral and axial resolution of a wide-field fluorescence microscope but has been too slow for live imaging. Here we apply 3D SIM to living samples and record whole cells at up to 5 s per volume for >50 time points with 120-nm lateral and 360-nm axial resolution. We demonstrate the technique by imaging microtubules in S2 cells and mitochondria in HeLa cells.Mesh:
Year: 2011 PMID: 22002026 DOI: 10.1038/nmeth.1734
Source DB: PubMed Journal: Nat Methods ISSN: 1548-7091 Impact factor: 28.547