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Literature DB >> 20693998

Live-cell super-resolution imaging with trimethoprim conjugates.

Richard Wombacher¹, Meike Heidbreder, Sebastian van de Linde, Michael P Sheetz, Mike Heilemann, Virginia W Cornish, Markus Sauer.

Abstract

The spatiotemporal resolution of subdiffraction fluorescence imaging has been limited by the difficulty of labeling proteins in cells with suitable fluorophores. Here we report a chemical tag that allows proteins to be labeled with an organic fluorophore with high photon flux and fast photoswitching performance in live cells. This label allowed us to image the dynamics of human histone H2B protein in living cells at approximately 20 nm resolution.

Entities: Chemical Species

Mesh：

Substances：
Histones
Trimethoprim

Year: 2010 PMID： 20693998 DOI： 10.1038/nmeth.1489

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

24 in total

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7. Super-resolution imaging with small organic fluorophores.

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8. Optimized fluorescent trimethoprim derivatives for in vivo protein labeling.

Authors: Nathaniel T Calloway; Michael Choob; Ana Sanz; Michael P Sheetz; Lawrence W Miller; Virginia W Cornish
Journal: Chembiochem Date: 2007-05-07 Impact factor: 3.164

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10. Kinetics of core histones in living human cells: little exchange of H3 and H4 and some rapid exchange of H2B.

Authors: H Kimura; P R Cook
Journal: J Cell Biol Date: 2001-06-25 Impact factor: 10.539

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6. Emerging Chemistry Strategies for Engineering Native Chromatin.

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8. Graphene-Enabled, Spatially Controlled Electroporation of Adherent Cells for Live-Cell Super-resolution Microscopy.

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10. Nano-fEM: protein localization using photo-activated localization microscopy and electron microscopy.

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