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Literature DB >> 21953185

Improving liquid chromatography-mass spectrometry sensitivity using a subambient pressure ionization with nanoelectrospray (SPIN) interface.

Keqi Tang¹, Jason S Page, Ioan Marginean, Ryan T Kelly, Richard D Smith.

Abstract

In this work, the subambient pressure ionization with nanoelectrospray (SPIN) ion source and interface, which operates at ~15-30 Torr, is demonstrated to be compatible with gradient reversed-phase liquid chromatography-MS applications, exemplified here with the analysis of complex samples (a protein tryptic digest and a whole cell lysate). A low liquid chromatographic flow rate (100-400 nL/min) allowed stable electrospray to be established while avoiding electrical breakdown. Efforts to increase the operating pressure of the SPIN source relative to previously reported designs prevented solvent freezing and enhanced charged cluster/droplet desolvation. A 5- to 12-fold improvement in sensitivity relative to a conventional atmospheric pressure nanoelectrospray ionization (ESI) source was obtained for detected peptides.

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Year: 2011 PMID： 21953185 PMCID： PMC3187566 DOI： 10.1007/s13361-011-0135-7

Source DB: PubMed Journal: J Am Soc Mass Spectrom ISSN： 1044-0305 Impact factor: 3.109

24 in total

1. Effect of different solution flow rates on analyte ion signals in nano-ESI MS, or: when does ESI turn into nano-ESI?

Authors: Andrea Schmidt; Michael Karas; Thomas Dülcks
Journal: J Am Soc Mass Spectrom Date: 2003-05 Impact factor: 3.109

Review 2. Ultrasensitive and quantitative analyses from combined separations-mass spectrometry for the characterization of proteomes.

Authors: Richard D Smith; Yufeng Shen; Keqi Tang
Journal: Acc Chem Res Date: 2004-04 Impact factor: 22.384

Review 3. Advanced nanoscale separations and mass spectrometry for sensitive high-throughput proteomics.

Authors: Yufeng Shen; Richard D Smith
Journal: Expert Rev Proteomics Date: 2005-06 Impact factor: 3.940

4. Efficiency of nano-electrospray ionization.

Authors: Ayman El-Faramawy; K W Michael Siu; Bruce A Thomson
Journal: J Am Soc Mass Spectrom Date: 2005-10 Impact factor: 3.109

5. Chemically etched open tubular and monolithic emitters for nanoelectrospray ionization mass spectrometry.

Authors: Ryan T Kelly; Jason S Page; Quanzhou Luo; Ronald J Moore; Daniel J Orton; Keqi Tang; Richard D Smith
Journal: Anal Chem Date: 2006-11-15 Impact factor: 6.986

6. Subambient pressure ionization with nanoelectrospray source and interface for improved sensitivity in mass spectrometry.

Authors: Jason S Page; Keqi Tang; Ryan T Kelly; Richard D Smith
Journal: Anal Chem Date: 2008-02-01 Impact factor: 6.986

7. A predictive model for matrix and analyte effects in electrospray ionization of singly-charged ionic analytes.

Authors: C G Enke
Journal: Anal Chem Date: 1997-12-01 Impact factor: 6.986

8. Analytical properties of the nanoelectrospray ion source.

Authors: M Wilm; M Mann
Journal: Anal Chem Date: 1996-01-01 Impact factor: 6.986

9. Achieving 50% ionization efficiency in subambient pressure ionization with nanoelectrospray.

Authors: Ioan Marginean; Jason S Page; Aleksey V Tolmachev; Keqi Tang; Richard D Smith
Journal: Anal Chem Date: 2010-10-28 Impact factor: 6.986

10. Physical/chemical separations in the break-up of highly charged droplets from electrosprays.

Authors: K Tang; R D Smith
Journal: J Am Soc Mass Spectrom Date: 2001-03 Impact factor: 3.262

11 in total

1. A new approach to high sensitivity liquid chromatography-mass spectrometry of peptides using nanoflow solvent assisted inlet ionization.

Authors: Beixi Wang; Ellen D Inutan; Sarah Trimpin
Journal: J Am Soc Mass Spectrom Date: 2012-01-12 Impact factor: 3.109

2. Fundamental Studies of New Ionization Technologies and Insights from IMS-MS.

Authors: Sarah Trimpin; Ellen D Inutan; Santosh Karki; Efstathios A Elia; Wen-Jing Zhang; Steffen M Weidner; Darrell D Marshall; Khoa Hoang; Chuping Lee; Eric T J Davis; Veronica Smith; Anil K Meher; Mario A Cornejo; Gregory W Auner; Charles N McEwen
Journal: J Am Soc Mass Spectrom Date: 2019-05-06 Impact factor: 3.109

3. Localization and imaging of gangliosides in mouse brain tissue sections by laserspray ionization inlet.

Authors: Alicia L Richards; Christopher B Lietz; James Wager-Miller; Ken Mackie; Sarah Trimpin
Journal: J Lipid Res Date: 2012-01-18 Impact factor: 5.922

4. Improving N-glycan coverage using HPLC-MS with electrospray ionization at subambient pressure.

Authors: Ioan Marginean; Scott R Kronewitter; Ronald J Moore; Gordon W Slysz; Matthew E Monroe; Gordon Anderson; Keqi Tang; Richard D Smith
Journal: Anal Chem Date: 2012-10-17 Impact factor: 6.986

5. Carrier-Assisted Single-Tube Processing Approach for Targeted Proteomics Analysis of Low Numbers of Mammalian Cells.

Authors: Pengfei Zhang; Matthew J Gaffrey; Ying Zhu; William B Chrisler; Thomas L Fillmore; Lian Yi; Carrie D Nicora; Tong Zhang; Huanming Wu; Jon Jacobs; Keqi Tang; Jacob Kagan; Sudhir Srivastava; Karin D Rodland; Wei-Jun Qian; Richard D Smith; Tao Liu; H Steven Wiley; Tujin Shi
Journal: Anal Chem Date: 2018-12-28 Impact factor: 6.986

6. Improving the sensitivity of mass spectrometry by using a new sheath flow electrospray emitter array at subambient pressures.

Authors: Jonathan T Cox; Ioan Marginean; Ryan T Kelly; Richard D Smith; Keqi Tang
Journal: J Am Soc Mass Spectrom Date: 2014-03-28 Impact factor: 3.109

10. High sensitivity combined with extended structural coverage of labile compounds via nanoelectrospray ionization at subambient pressures.

Authors: Jonathan T Cox; Scott R Kronewitter; Anil K Shukla; Ronald J Moore; Richard D Smith; Keqi Tang
Journal: Anal Chem Date: 2014-09-26 Impact factor: 6.986