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Literature DB >> 21941297

miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2.

Nils Pfaff¹, Jan Fiedler, Angelika Holzmann, Axel Schambach, Thomas Moritz, Tobias Cantz, Thomas Thum.

Abstract

Induced pluripotent stem cells (iPSCs) can be generated by overexpression of Oct4, Sox2 and Klf4 in murine fibroblasts. By conducting a microRNA (miRNA) library screen, we identified a set of miRNAs critically regulating iPSC formation. We revealed a new miRNA family (miR-130/301/721) as an important regulator of iPSC induction by targeting the homeobox transcription factor Meox2 (also known as Gax). Meox2-specific silencing mimicked the effects of this miRNA family on reprogramming. Mechanistically, miRNA-resistant Meox2 overexpression abrogated effects of miR-130/301/721 on reprogramming. In conclusion, the miRNA family miR-130/301/721 enhances iPSC generation via repression of Meox2.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 2011 PMID： 21941297 PMCID： PMC3207101 DOI： 10.1038/embor.2011.176

Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807

27 in total

1. Allele-specific expression of imprinted genes in mouse migratory primordial germ cells.

Authors: Piroska E Szabó; Karin Hübner; Hans Schöler; Jeffrey R Mann
Journal: Mech Dev Date: 2002-07 Impact factor: 1.882

2. The homeobox gene Gax inhibits angiogenesis through inhibition of nuclear factor-kappaB-dependent endothelial cell gene expression.

Authors: Sejal Patel; Alejandro D Leal; David H Gorski
Journal: Cancer Res Date: 2005-02-15 Impact factor: 12.701

3. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells.

Authors: Deepa Subramanyam; Samy Lamouille; Robert L Judson; Jason Y Liu; Nathan Bucay; Rik Derynck; Robert Blelloch
Journal: Nat Biotechnol Date: 2011-04-13 Impact factor: 54.908

4. Bax-mediated cell death by the Gax homeoprotein requires mitogen activation but is independent of cell cycle activity.

Authors: H Perlman; M Sata; A Le Roux; T W Sedlak; D Branellec; K Walsh
Journal: EMBO J Date: 1998-07-01 Impact factor: 11.598

5. Lentiviral vector design and imaging approaches to visualize the early stages of cellular reprogramming.

Authors: Eva Warlich; Johannes Kuehle; Tobias Cantz; Martijn H Brugman; Tobias Maetzig; Melanie Galla; Adam A Filipczyk; Stephan Halle; Hannes Klump; Hans R Schöler; Christopher Baum; Timm Schroeder; Axel Schambach
Journal: Mol Ther Date: 2011-02-01 Impact factor: 11.454

6. MicroRNA cluster 302-367 enhances somatic cell reprogramming by accelerating a mesenchymal-to-epithelial transition.

Authors: Baojian Liao; Xichen Bao; Longqi Liu; Shipeng Feng; Athanasios Zovoilis; Wenbo Liu; Yanting Xue; Jie Cai; Xiangpeng Guo; Baoming Qin; Ruosi Zhang; Jiayan Wu; Liangxue Lai; Maikun Teng; Liwen Niu; Biliang Zhang; Miguel A Esteban; Duanqing Pei
Journal: J Biol Chem Date: 2011-03-22 Impact factor: 5.157

7. Oct4 distribution and level in mouse clones: consequences for pluripotency.

Authors: Michele Boiani; Sigrid Eckardt; Hans R Schöler; K John McLaughlin
Journal: Genes Dev Date: 2002-05-15 Impact factor: 11.361

8. Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency.

Authors: Frederick Anokye-Danso; Chinmay M Trivedi; Denise Juhr; Mudit Gupta; Zheng Cui; Ying Tian; Yuzhen Zhang; Wenli Yang; Peter J Gruber; Jonathan A Epstein; Edward E Morrisey
Journal: Cell Stem Cell Date: 2011-04-08 Impact factor: 24.633

9. Regulation of somatic cell reprogramming through inducible mir-302 expression.

Authors: Shi-Lung Lin; Donald C Chang; Chun-Hung Lin; Shao-Yao Ying; Davey Leu; David T S Wu
Journal: Nucleic Acids Res Date: 2010-09-24 Impact factor: 16.971

10. Small RNA-mediated regulation of iPS cell generation.

Authors: Zhonghan Li; Chao-Shun Yang; Katsuhiko Nakashima; Tariq M Rana
Journal: EMBO J Date: 2011-02-01 Impact factor: 11.598

37 in total

Review 1. miRNAs involved in the generation, maintenance, and differentiation of pluripotent cells.

Authors: Nils Pfaff; Thomas Moritz; Thomas Thum; Tobias Cantz
Journal: J Mol Med (Berl) Date: 2012-06-09 Impact factor: 4.599

2. Stem cell biology--from basic research to regenerative medicine.

Authors: Daniel Besser
Journal: J Mol Med (Berl) Date: 2012-07 Impact factor: 4.599

3. Growth arrest-specific homeobox is associated with poor survival in patients with hepatocellular carcinoma.

Authors: Peng Zhou; Zhi Chen; Rui-Min Chang; Wei Jiang; Lie-Lin Wu; Zhi-Ming Wang
Journal: Med Oncol Date: 2012-05-27 Impact factor: 3.064

Review 4. An Insight into DNA-free Reprogramming Approaches to Generate Integration-free Induced Pluripotent Stem Cells for Prospective Biomedical Applications.

Authors: Manash P Borgohain; Krishna Kumar Haridhasapavalan; Chandrima Dey; Poulomi Adhikari; Rajkumar P Thummer
Journal: Stem Cell Rev Rep Date: 2019-04 Impact factor: 5.739